• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Forensic Applications of ELISA

Research Project

Project/Area Number 62304039
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionMedical College of Oita

Principal Investigator

TAMAKI Yoshihiro  Medical College of Oita, Professor, 医学部, 教授 (20028377)

Co-Investigator(Kenkyū-buntansha) KATSUMATA Yoshinao  Nagoya University School of Medicine, Professor, 医学部, 教授 (30109326)
MUKOYAMA Harutaka  National Research Institute of Police Science, Lab. Director, 法医第2研究室, 室長
IKEMOTO Shigenori  Jichi Medical School, Professor, 医学部, 教授 (90048942)
SAGISAKA Kaoru  Tohoku University School of Medicine, Professor, 医学部, 教授 (70006740)
TAKATORI Takehiko  Hokkaido University School of Medicine, Professor, 医学部, 教授 (30001928)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1988: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsELISA / Personal identification / Identification of human body fluids and stains / Differntial ABO grouping of semen in mixed stains / GM typing / 混合体液のABO型判定 / GM型判定 / パラコートの微量定量 / エライザ / 血痕検査 / 体液斑検査 / 血液型検査 / 種属鑑別 / Gm型判定 / 体液のABO型判定 / 人尿斑の同定 / ドットELISA
Research Abstract

1. Monoclonal antibodies (MoAbs) to ABO and MN antigens, glycophorin A and B, IgG allotypes G3M G and T, A2-seminoglycoprotein (A2SGP), and paraquat were produced for use in ELISAs.
2. Human blood could be identified by inhibition-ELISAs using ALB, IgG, A2M, HP, TF, GC, A2HS, BF, and HF as indicator proteins. Human urinary stains were best identified by a capture ELISA using anti-uromucoid as the capture antibody.
3. Automation and data processing for ABO grouping of body fluids were effected by assembly of a reaction unit containing a plate coated with stain-derived, detergent-solubilized antigen, and an automatic reading unit.
4. A simple dot-immunobinding (dot-ELISA) method for GM typing was developed using MoAbs to G3M G and G3M T. The method is well suited for routine forensic casework.
5. A competitive ELISA for paraquat was developed which enabled determination of 1-10 ng/ml of the pesticide.
6. Saliva, semen, and blood in mixed stains could be differentially ABO grouped by capture ELISAs using corresponding antibodies. In particular, home-made MoAbs to AB-carrying A2SGR specifically captured the corresponding protein, thereby enabling correct ABO grouping of semen mixed with vaginal fluids. Thus the method appears useful in rape casework.
7. Production of MoAbs to IgG allotypes GlM F and GlM X is in progress, since four GM monoclonals are required for routine GM typing of Japanese.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report

Research Products

(58 results)

All Other

All Publications (58 results)

  • [Publications] 池本卯典: Report of 15th Forensic Seminar in Korea,1987. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 向山明孝: モダンメディシン. 16. 44-51 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 堤肇: Journal of Forensic Sciences. 33. 237-243 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Tsutsumi: Acta Criminologica Japonica. 54. 163-168 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 勾坂馨: 法医学の実際と研究. 31. 51-54 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Kitano: British Journal of Hematology. 69. 329-333 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Kishida: Zeitschrift fur Rechtsmedizin. 101. 15-20 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Sagisaka: Acta Criminologica Japonica. 55. 8-12 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] A.Kimura: Journal of Immunological Methods. 116. 99-103 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Nagao: Clin.Chem.Enzym.Comms.3. (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Mukoyoma: Proc.3rd Symposium on Techniques of Identifying Persons and Handling Evidence,I.C.P.O.(1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] K.Sagisaka: Forensic Science International. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] E.Kajii: Vox Sanguinis. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Idemoto: Vox Sanguinis. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] D.Nadano: Carbohydrates. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.Kishida: Forensic Science International. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Nagao: Human Toxicology. 8. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M.Nagao: Journal of Immunoassay. 10. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ikemoto, S.: "Blood Grouping of Bloodstains with Monoclonal Antibodies" Report of 15th Forensic Seminar in Korea, 1987. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mukoyama, H.: "Biryo Kekkon karano Ketsuekigata Jido Hantei (in Japanese)" Modern Medicine. 16. 44-51 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsutsumi, H.: Identification of Human Urinary Stains by Enzyme-Linked Im munosorbent Assay for Human Uromucoid. 33. 237-243 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tsutsumi, H.: "Identification of human Urinary Stains by Enzyme-Linked Immunosorbent Assay(ELISA) Using Rabbit Anti-Human Uromucoid" Acta Criminologica Japonica. 54. 163-168 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sagisaka, K.: "Kairi-ho-ELISA niyoru Ketsuekigata Kensa" Hoigaku no Jissai to Kenkyu. 31. 51-54 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kitano, K.: "Confirmation of Bone Marrow Engraftment by Detection of M/N Blood Group Antigens on Erythroblasts in Erythroid Bursts" British journal of Hematology. 69. 329-333 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kajii, E.: "Characterization of the Monoclonal Crossreacting Anti-A,B Antibody" Vox Sanguinis.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kishida, T.: "G3m(21) Typing by ELISA and Dot Immunobinding with Enzyme-Labeled Monoclonal Anti-G3m(21) Antibody" Zeitschrift fur Rechtsmedizin. 101. 15-20 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sagisaka, K.: "The Use of Deoxy-BIGCHAP to Extract ABH Antigens from Bloodstains for Blood Grouping by ELISA" Acta Criminologica Japonica. 55. 8-12 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kimura, A.: "Production of Monoclonal Antibody to Human IgG Allotype G3M T" Journal of Immunological Methods. 116. 99-103 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nagao, M.: "Enzyme-Linked Immunosorbent Assay for Paraquat and its Toxicological Application" Clin. Chem. Enzym. Comms.3. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mukoyama, H.: "Automation and data processing for identification of Biological Evidences in Foresic Science Laboratory" Proc. 3rd Symposium on Techniques of identifying Persons and Handling Evidencje, International Criminal police organization. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sagisaka, K.: "Serologically Specific Capture of ABH Blood Group Antigens of the red Cell or Body Fluids by Double Antibody Sandwich ELISA" Forensic Science International. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ikemoto, S.: "Detection of Lewis Blood group Antigens in Human Saliva by Monoclonal Anti-Le^a and Anti-Le^b Antibodies which Were Produced wigh Blood group Substance Derived from Saliva as Antigens" Vox Sanguinis. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nadano, D.: "Purification and Characterization of Human H and Le^b-Active Salivary Mucin" Carbohydrates. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kishida, T.: "Production of Monoclonal Antibody to ABH-Carrying A2-Seminoglycoprotein (A2-SGP) for ABO Grouping of Semen by ELISA" Forensic Science International.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nagao, M.: "Immunotherapy for the Treatment of Acute Paraquat Poisoning" Human Toxicology. 8. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nagao, M.: "Development and Characterization of monoclonal Antibodies Reactive with Paraquat" Journal of Immunoassay. 10. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M,Nagao.: Human Toxicology. 8. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M,Nagao.: Journal of Immunoassay. 10. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] M,Nagao.: Journal of Forensic Sciences. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] M,Nagao.: Clin.Chem.Enzym.Comms.3. (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 匂坂馨: 法医学の実際と研究. 31. 51-54 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] K,Sagisaka.: Acta Criminogica Japonica. 55. 8-12 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K,Sagisaka.: Forensic Science International. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] E,Kajii.: Vox Sanguinis. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] S,Ikemoto.: Vox Sanguinis. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] K,Kitano.: British Journal of Hematology. 69. 329-333 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] D,Nadano.: Carbohydrates. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] H,Mukoyama.: Proc.3rd Symposium on Techniques of Identififying Persons and Handling Evidence,I.C.P.O.(1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H,Tsutsumi.: Acta Criminologica Japonica. 54. 163-168 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] T,Kishida.: Zeitschrift fur Rechtsmedizin. 101. 15-20 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A,Kimura.: Journal of Immunological Methods. 116. 99-103 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T,Kishida.: Forensic Science International. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高取健彦: Toxicology.

    • Related Report
      1987 Annual Research Report
  • [Publications] 池本卯典: Report of 15th Forensic Seminar in Korea. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 池本卯典: Journal of Forensic Medicine.

    • Related Report
      1987 Annual Research Report
  • [Publications] 向山明孝: モダン・メディシン. 16. 44-51 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 岸田哲子: Zeitschrift fiir Rechtsmedizin. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 堤肇: Journal of Forensic Sciences. 33. 237-243 (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1988-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi