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The Role of Microorganisms in Periodontal Diseases

Research Project

Project/Area Number 62304049
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOkayama University

Principal Investigator

MURAYAMA Yoji  Okayama Univ. Dent. Sch. Professor, 歯学部, 教授 (50029972)

Co-Investigator(Kenkyū-buntansha) EBISU Shigeyuki  Osaka Univ. Dent. Sch. Associate Professor, 歯学部, 助教授 (50116000)
OKAMOTO Hiroshi  Hiroshima Univ. Dent. Sch. Professor, 歯学部, 教授 (50028742)
MAEDA Katsumasa  Kyushu Univ. Dent. Sch. Associate Professor, 歯学部, 助教授 (00117243)
AONO Masao  Kyushu Univ. Dent. Sch. Professor, 歯学部, 教授 (70037498)
KATO Keijiro  Okayama Univ. Dent. Sch. Professor, 歯学部, 教授 (50028718)
青野 正男  九州大学, 歯学部, 教授 (27037498)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥18,200,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1987: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsPeriodontal disease / Bacterial flora / Specific antigen / Fimbriae / Recombinant antigen / Binding mechanism of bacteria / Bacterial mechanism / Periodontal disease activity / 線毛抗原 / 生体の殺菌機構 / 歯周病の微生物叢 / 歯周病細菌の特異抗原 / 歯周病の病態 / 歯周病の診断
Research Abstract

1.Bacterial ecology in periodontal pockets : (1)The approaches to examination of the specific microorganisms within periodontal pockets were performed through indirect immunofluorescence procedure, enzyme-labeled antibody test and DNA-DNA hybridization method (Ebisu, Murayama, Hara).(2) A method for routine culturing of oral spirochetes was established (Ishikawa). (3)The nutritional relationship among periodontal microorganisms were examined on a mixed culture of Wolinella reeta, a periodontal bacteria, and Streptococcus sanguis. The mechanisms of bacterialsucce ssion in periodontal pockets were discussed. 2.Bacterial components and their biological activities : (1)A colonial variation in Actinobacillus actinomycetemcomitans seems to relate the presence of outermembrane proteins (fimbrite) on the cells (Kato). (2)The biological activities of Selenomonas sputigena and Bacteroides gingivalis(Bg) were examined (Usemoto). (3)Binding specificity of periodontal bacteria to tissue cells and b … More acterial cells were studiedabout Mycoplasma salivarium (Watanabe) and Fusobacterium nucleatum (Okamoto). 3.The specific antigens from Bg : An antigen from Bg 381 was characterized by the manner of humoral immune response in patients with periodontitis and purified (Murayama). (2)The gene encoding the fimbrial subunit protein from Bg 381 (fimbrilin) was cloned and sequenced. Then it was used forclini- cal detection of the Bg strains in periodontal pockets (Yoshimura, Suzuki). (3)Gene banksof the chr omosomal DNA from Bg 381 were constructed. The clone product was used for further studies of Bg (Abiko). 4.Host response against periodontal bacteria : (1)Lactoferrin was related to the bactericidal mechanism in periodontal pockets (Maita, Endo). (2)Bg released sub-stanc es which suppress the functions of polymorphonuclear leukocytes via the modulation of cellsurface re ceptors and internal cellular events (Aono, Maeda). 5.Diagnosis and bacterial flora : (I)The correl ation among antibody levels to bacteria, clinical status, and bacterial flora inperiodontal pockets were examined. Then the diagnosis of periodontal diseases were discussed from the results (Hara). (2)The diagnostic methods for measurement of periodontal disease activity were synthetically searched from the bacterial, biochemical, immunological, and clinical aspects (Ebisu). Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (83 results)

All Other

All Publications (83 results)

  • [Publications] Kumada,H.: "Chemical and biological properties of lipopolysaccharide,lipid A and degraded polysaccharide from Wolinella recta ATCC 33238." J.Gen.Microbiol.135. 1017-1025 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kokeguchi,S.: "Cell surface antigen from Wolinella recta ATCC 33238." J.Clin.Microbiol.27. 1210-1217 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohta,H.: "Interーrelationships between Wolinella recta and Streptococcus sanguis." Microbial Ecology in Health and Disease(投稿予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Umeda,M.: "Improved detection of oral spirochetes with an anaerobic culture method." Oral Microbiol.Immunol.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川恵里子: "Bacteroides gingivalis の血清型分類およびクル-プ特異抗原の精製" 歯基礎誌. 31. 647-655 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 渡辺清子: "Selenomonas sputigena 菌体成分の免疫生物活性について" 歯基礎誌(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Murayama,Y.: "Serum immunoglobulin G antibody to periodontal bacteria" Adv.Dent.Res.2. 339-345 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Murayama,Y.: "Local administration of minocycline in periodontal disease:Double blind comparative study of LSー007." Recent Advances in Clinical Periodontology. 215-218 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishimura,F.: "A family study of a mother and daugher with increased susceptibility to earlyーonset periodontitis:microbiological,immunological,host defensive,and genetic analysis." J.Periodontol.(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,T.: "Detection of fimbriae and fimbrial antigens on the oral anaerobe Bacteroides gingivalis by negative staining and serological methods" J.Gen.Microbiol.134. 2713-2720 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki,T.: "Fimbriae from the oral anaerobe Bacteroides gingivalis:A screaning of clinical isolates from various places." Adv.Dent.Res.2. 301-303 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshimura,F.: "Purification and properties of a 75ーkilodalton major protein,an immunodominant surface antigen,from the oral anaerobe Bacteroides gingivalis." Infect.Immun.57. 3646-3652 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abiko,Y.: "Gene cloning of a Bacteroides gingivalis specific protein antigen and Escherichia coli." Adv.Dent.Res.2. 310-314 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abiko,Y.: "Cloning of a Bacteroides gingivalis outer mambrane protein in Escherichia Coli." (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hayakawa,M.: "Gene cloning of Bacteroides gingivalis antigens recogized by serum of periodontitis." (投稿準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeda,K.: "The modulation of polymorphonuclear leukocyte′s function by Bacteroides gingivalis 381." Recent Advances in Clinical Periodontology. 375-378 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeda,K.: "The modulation of polymorphonuclear leukocyte function by Bacteroides gingivalis." Adv.Dent.Res.2. 315-318 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ozaki,M.: "Binding Specificity of Fusobacterium nucleatum human erythrocytes,polymorphonuclear leukocyter,fibroblasts,and Hela to cells." J.Periodont.Res.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibata,K.: "Purification and characterization of an arginineーspecificcarboxpeptidase from Mycoplasma salivarium." J.Bacteriol.55. 263-265 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibata,K.: "Inactivation of the vascular permeabilityーincreasing activity of bradykinin by mycoplasmas." FEMS Microbiol.Lett.69. 149-152 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Watanabe,T.: "Hemagglutinating activity of Mycoplasma salivarium and its attachment to sheep red blood cells." (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 安孫子宜光: "歯周病ワクチンへの遺伝子工学利用" 歯科ジャ-ナル. 28. 488-496 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 奥田一博: "歯肉縁下細菌叢の変動および臨床指標の変動から予測される歯周ポケットの深さ" 日歯周誌. 30. 672-699 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 遠藤英昭: "歯周病関連菌に対するラクトフェリンの作用" 日歯周誌(投稿準備中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kumada, H.: "Chemical and biological properties of lipopolysaccharide, lipid A and degraded polysaccharide from Wolinella recta ATCC 33238." J. Gen. Microbiol. 135. 1017-1025 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kokeguchi, S.: "Cell surface antigen from Wolinella recta ATCC 33238." J. Clin. Microbiol. 27:1210-1217, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohta, H.: "Inter-relationships between Wolinella recta and Streptococcus sanguis." Microbial Ecology in Health and Disease.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Umeda, M.: "Improved detection of oral spirochetes with an anaerobic culture method." Oral Microbiol. Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Umemoto, T.: "Serological classification of Bacteroides gingivalis and serogroup distribution in periodontitis patients." Dentistry in Japan (1988-1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Murayama, Y.: "Serum immunoglobulin G antibody to periodontal bacteria." Adv. Dent. Res. 2 : 339-345, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Murayama, Y.: "Local administration of minocycline in periodontal disease : Double blind comparative study of LS-007." Recent Advances in Clinical Periodontology 215-218, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Detection of fimbriae and fimbrial antigens on the oral anaerobe Bacteroides gingivalis by negative staining and serological methods." J. Gen. Microbiol. 134 : 2713-2720, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Fimbriae from the oral anaerobe Bacteroides gingivalis: A screening of clinical isolates from various places." Adv. Dent. Res. 2 : 301-303, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoshimura, F.: "Purification and properties of a 75-kilodalton major protein, an immunodominant surface antigen, from the oral anaerobe Bacteroides gingivalis." Infect. Immun. 57 : 3646-3652, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abiko, Y.: "Gene cloning of a Bacteroides gingivalis specific protein antigen in Escherichia coli." Adv. Dent. Res. 2 : 310-314, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Abiko, Y.: "Cloning of a Bacteroides gingivalis outer membrane protein in Escherichia coli."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hayakawa, M.: "Gene cloning of B. gingivalis antigens recognized by serum of periodontitis."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeda, K.: "The modulation of polymorphonuclear leukocyte's function by Bacteroides gingivalis 381." Recent Advances in Clinical Periodontology 375-378, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeda, K.: "The modulation of polymorphonuclear. leukocyte function by Bacteroides gingivalis." Adv. Dent. Res. 2 : 315-318, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yoneda, M.: "Suppression of boctericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis." Infect. Immun.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ozaki, M.: "Binding specificity of Fusobacterium nucleatum human erythrocytes, polymorphonuclear leukocytes, fibroblasts, and Hela to cells." J. Periodont. Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibata, K.: "Purification and characterization of an arginine-specific carboxypeptidase from Mycoplasma salivarium." J. Bacteriol. 55 : 263-265, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shibata, K.: "Inactivation of the vascular permeability-increasing activity of bradykinin by mycoplasmas." FEMS Microbiol. Lett. 69:49-152, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Watanabe, T.: "Hemagglutinating activity of Mycoplasma salivarium and its attachment to sheep red blood cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Horino, K.: "Correlation between serum and gingival crevicular fluid antibodies and periodontal status." J. Dent. Res. 68 : 1688-1690, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kumada,H.: "Chemical and biological properties of lipopolysaccharide,lipid A and degraded polysaccharide from Wolinella recta ATCC 33238." J.Gen.Microbiol.135. 1017-1025 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kokeguchi,S.: "Cell surface antigen from Wolinella recta and ATCC 33238." J.Chin.Microbiol.27. 1210-1217 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石川恵里子: "Bacteroides gingivalis血清型分類およびグル-プ特異抗原の精製" 歯基礎誌. 31. 648-655 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nishikata,M.: "Possibility of Bacteroides gingivalis Hemagglutinin possenssing protease activity revealed by inhibition studies." Microbiol.Immunol.33. 75-80 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshimura,F.: "Purification and properties of a 75-kilodalton major protein,an immunodominant surface antigen,from the oral anaerobe Bacteroides gingivalis." Infect.Immun.57. 3646-3652 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shibata,K.: "Inactivation of the vascular permeability-increasing activity of bradykinin by mycoplasmas." FEMS Microbiol.Lett.69. 149-152 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoneda,M.: "Suppression of bactericidal activity of human polymorphnuclear leukocytes by Bacteroides gingivalis." Infect.Immun.

    • Related Report
      1989 Annual Research Report
  • [Publications] Horino,K.: "Correlation between serum and gingival crevicular fluid antibodies and periodontal status." J.Dent.Res.68. 1688-1690 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Umeda,M.: "Improved detection of oral spirochetes with an anaerobic culture method." Oral.Microbiol.Immunol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Umemoto,T.: "Serological classification of Bacteroides gingivalis and serogroup distribution in periodontitis patients." Dentistry in Japan.

    • Related Report
      1989 Annual Research Report
  • [Publications] 渡辺清子: "Selenomonas sputigene菌体成分の免疫生物活性について" 歯基礎誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 水島ゆみ: "歯周病関連運動性微生物に対する体液性免疫応答" 岡山歯誌. 8. 65-74 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田寛行: 岡山歯学会雑誌. 7. 1-16 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,Y.: Adv.Dent.Res.2. 301-303 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Suzuki,Y.: J.Gen.Microbiol.134. 2713-2720 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Isogami,H.: Arch.Oral Biol.33. 479-485 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Dickinson,D.P.: J.Bacteriol.170. 1658-1665 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Abiko,Y.: Adv.Dent.Res.2. 310-314 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 柴田健一郎: 第15回日本マイコプラズマ学会記録. 73-76 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小林浩明: 第15回日本マイコプラズマ学会記録. 69-72 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 中永大喜: 第15回日本マイコプラズマ学会記録. 65-68 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shibata,K.: J.Bacteriol.170. 1795-1799 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ishikawa,I.: Adv.Dent.Res.2. 334-338 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Noguchi,K.: Prostaglandins Leukotrienes and Essential Fatty Acids. 33. 137-141 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Maeda,K.: Adv.Dent.Res.2. 315-318 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 奥田一博: 日歯周誌. 30. 672-699 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Murayama,Y.: Adv.Dent.Res.2. 339-345 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Mizushima,Y.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 673-676 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kobayashi,K.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 681-684 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Shibata,K.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 685-688 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Watanabe,T.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 405-408 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Maeda,K.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 375-378 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Endo,H.: "Recent Advances in Clinical Periodontology" Elsevier Science Publishers B.V., 657-660 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hiroyuki Ohta: Microbiol.Immunol.31. 313-325 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fuminobu Yoshimura: Microbiol.Immunol.31. 935-941 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 鈴木武: 北海道歯学雑誌. 8. 15-16 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 吉村文信: 歯界展望. 69. 1463-1474 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tsuguo WAtanabe: 35th Annual Meeting of JADR Program and Abgtracts of Papers. Special. 46 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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