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RECONSTRUCTION OF BIOMEMBRANE MOLECULES IN PLANAR LIPID BILAYER MEMBRANES

Research Project

Project/Area Number 62304061
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionOSAKA UNIVERSITY

Principal Investigator

TAKAGI MASAYUKI  OSAKA UNIV. Associate Professor, 教養部, 助教授 (20028145)

Co-Investigator(Kenkyū-buntansha) KASAI MICHIKI  OSAKA UNIV. Professor, 基礎工学部, 教授 (40022595)
藤墳 規明  名古屋工業大学, 助教授 (60101268)
HOSHI TAKECHI  UNIV. OF SHIZUOKA, Professor, 食品栄養科学部, 教授 (60004537)
SUGIYAMA HIROYUKI  NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES, Associate Professor, 助教授 (20124224)
TSUDA MOTOYUKI  SAPPORO MEDICAL COLLEGE, Assistant Professor, 講師 (60045458)
KIRINO YUTAKA  KYUSHU UNIV. Professor
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 1988: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1987: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsPlanar Lipid Bilayer Membrane / Ion Channels in Photoreceptor / Reconstruction of K^+-ehannel / 巨大ベシクル膜 / パッチング / アフリカツメガエルの卵母細胞 / グルタミン酸受容チャネル / 平面膜 / 視細胞イオンチャンネル / 巨大リポゾーム / Ca^<++>ポンプ / 視細胞イオンチャネル / 筋小包体アニオンチャネル / アフリカツメガエル卵母細胞 / 塩素イオンチャネル / Gタンパク質 / プロトンポンプ分子 / リシン輸送
Research Abstract

1) Identification and Purification of Ion Channels in Invertebrate (Octopus) Photoreceptor Membranes by Means of Planar Lipid Bilayer Membrane: The microvilli membrane of octopus photoreceptor were isolated, and fractionated into several protein components. Of these, two showed definite channel activities in record of their planar lipid bilayer membrane currents. Their conductances were 150 pS and 10 pS. The ion channel of 150 pS has no selectivity between K^+ and Na^+ and is permeable to the large organic cations, such as choline^+ and tetraethylammonium^+.
2) Reconstruction of K^+-channel in Planar Lipid Bllayer Membrane:Roughly purified K^+-channel proteins obtained from muscle sarcomic reticulum were reconstructed in a giant liposome of azolectin, which was patched with fine glass pipet ( =0.5um). An analysis on the single K^+-channel current on the patched membrane showed that the channel has a substate and the gating system is controlled by K^+ ion istelf.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takagi,M.: Photochemistry and Photobiology. 45. 651-656 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sokabe,M.: Brain Research. 443. 245-260 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirata,H.: Develop.Growth Differ.30. 407-407 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sugiyama,H.: Biomed.Res.8. 55-58 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hoshi,T.: Advances in Biosciences. 65. 115-120 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fujitsuika,C.: J.Neurocytol.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高木雅行: "図説 情報生物科学" 裳華房, 144 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takagi, M., et al.: "X-ray Microanalysis of the Effect of Illumination on the Ionic Composition of the Octopus Photoreceptor." Photochemistry and Photobiology. 45. 651-656 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sokabe, M.,;Fujitsuka, N., et al.: "Effects of Cyclic Nucleotides and Calcium on Transduction and Encoding Process in Frog Muscle Spindle." Brain Research. 443. 254-260 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirata, H., et al.: "Structure and Function of Calcium Channel." Develop. Growth Differ.30. 407-407 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sugiyama, H., et al.: "Functional Properties of Neurotransmitter Receptors Transplanted into Xenopus Oocytes." Biomed. res.8. 55-58 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hoshi, T., et al.: "Stoichiometry and Specificity of the Proton/Peptide Cotransport across Intestinal Brush Border Membranes." Advances in Biosciences. 65. 115-120 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takagi, M.: Informational Bioscience-Illustrated. Asakura Shoten (Tokyo), 114 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirata,H.: Develop.Growth Differ.30. 407-407 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sokabe,M: Brain Res.443. 254-260 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fujitsuka,C.: J.Neurocytol.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sugimoto,N.: Biochim.Biophys.Res.Com.45. 551-560 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hirashima,N.: Biochim.Biophys.Acta. 946. 209-214 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hoshi,T.: Bifidobact.Microflora. 7. 29-34 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 葛西道生 他編: "神経情報伝達分子" 培風館, 184 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高木雅行: "図説情報生物科学" 裳華房, 144 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高木雅行: Photochemistry and Photobiology. 45. 651-656 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 杉山博之: Nature. 352. 531-533 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 星猛: Proceedings of the First Congress of the Asian and Oceanian Physiological Societies. 111-117 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 藤墳規明: Journal of Neurocytology. 16. 185-194 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Edt.by Ovchinnikov;津田基之: "Retinal Proteins" VNU Science Press,The Netherlands, 393-404 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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