| Project/Area Number |
62304062
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Osaka University |
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Principal Investigator |
KURODA Kiyoko Osaka Univ. Fac. Sci. Assoc. Prof., 理学部, 助教授 (30028138)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Harunori Gunma Univ. Fac. Med. Prof., 医学部, 教授 (90010058)
NAGAI Reiko Osaka Univ. Gen. Edu. Prof., 教養部, 教授 (90028192)
YOSHIMOTO Yasuaki Kansai Med. Univ. Prof., 教授 (30124225)
YONEDA Mitsuki Kyoto Univ. Fac. Sci. Prof., 理学部, 教授 (50017183)
ISHIGAMI Mitsuo Shiga Univ. Fac. Edu. Assoc. Prof., 教育学部, 助教授 (20111883)
伊豆津 公作 三重大学, 医学部, 教授 (50024617)
黒田 英世 名古屋大学, 理学部, 助教授 (50064845)
佐藤 英美 名古屋大学, 理学部, 教授 (40109260)
秦野 節司 名古屋大学, 理学部, 教授 (50022570)
上坪 英治 一橋大学, 商学部, 教授 (60111697)
新免 輝男 東京大学, 理学部, 助教授 (80114510)
馬渕 一誠 東京大学, 教養学部, 助教授 (40012520)
毛利 秀雄 東京大学, 教養学部, 教授 (70012268)
渡辺 良雄 筑波大学, 生物科学系, 教授 (00015918)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥16,400,000 (Direct Cost: ¥16,400,000)
Fiscal Year 1989: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1988: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1987: ¥8,700,000 (Direct Cost: ¥8,700,000)
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| Keywords | Cell Motility / Cytoskeleton / Cell Division / Actin / Myosin / Tubulin / Dynein / Calcium / チュ-グリン / チューブリン |
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| Research Abstract |
Various movement in non-muscle cells associates with remarkable change in cell shapes. Cytoskeletons in migrating cells repeat a rapid construction-> destruction->transportation-> reconstruction cycle in appropriate part of the cells. The present studies made efforts to gain further insight into the regulatory mechanism of the cytoskeleton in relation to cell motility.
Ishigami studied dynamic aspects of the contractile system in Physarum plasmodium. Hatano analysed cyclic generation-degeneration of an individual cytoplasmic actomyosin fibril. Fine structures and protein components of stress fiber-like structures in isolated renal tubules were clarified by Ishikawa. Watanabe purified Tetrahymena actin and its binding proteins and investigated their function in living cells. By biochemical studies of actin modulating proteins and fine structural analysis of cleavage furrow formation in sea urchin eggs, Mabuchi clarified molecular mechanism of cell division. Sato investigated dynamic structure of spindle microtubules and chromosomal movement. Using dynein-antibodies, Izutsu revealed an association of a cytoplasmic dynein-like protein with mammalian mitotic spindles. Mohri showed dynamic conformational change of 21S outer arm dynein coupled with ATP hydrolysis. Kuroda,H. studied the Ca^<2+> dynamics during fertilization of sea urchin eggs. With microinjected aequorin, Yoshimoto revealed temporal and spatial localization of Ca^<2+> in Physarum, Amoeba and Paramecium. With the video centrifuge microscope Kuroda,K. calculated propulsive force of single Paramecium. In characean internodal cells, Kamitsubo considered the viscosity of cytoplasm contiguous to the subcortical fibrils, and Yoneda tried hydrodynamic analysis of the cytoplasmic streaming. Shimmen studied the molecular mechanism of cytoplasmic streaming in lily pollen tubes and Nagai showed light-induced reorganization of microtubule bundles in Dichotomosiphon.
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