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Basic Studies on Evolution Machines

Research Project

Project/Area Number 62420055
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionSaitama University

Principal Investigator

HUSIMI Yuzuru  Saitama Univ., Dept. of Environ. Chem., Prof., 工学部, 教授 (80011641)

Co-Investigator(Kenkyū-buntansha) KIHARA Hiromu  Saitama Univ., Dept. of Environ. Chem., Res. Assoc., 工学部, 助手 (90204958)
IIDA Takeaki  Saitama Univ., Dept. of Appl. Chem., Prof., 工学部, 教授 (30013171)
NISHIGAKI Koichi  Saitama Univ., Dept. of Environ. Chem., Assoc. Prof., 工学部, 助教授 (10107378)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥34,100,000 (Direct Cost: ¥34,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 1987: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsEVOLUTIONARY MOLECULAR ENGINEERING / EVOLUTION REACTOR / CELLSTAT / CONTINUOUS CULTURE OF BACTERIOPHAGE / BASE SEQUENCE SPACE / SELECTIVE VALUE LANDSCAPE / PCR / CELL-SORTER / 進化機械 / 進化リアクター / バクテリオファージ連続培養 / 淘汰値の地形 / 遺伝子のランダム突然変異誘発 / 自由淘汰値
Research Abstract

1. Development of an artificial selection-type evolution machine. (1) A cloning system for a specified gene which is inserted into fd DNA has been developed. A specially modified cell-sorter can put a carrier-cell into a well of 1000-well microplate without the free background phage. (2) A quantitative screening method for a cloned mutant gene(lac promoter) has been demonstrated. A fluorogenic substrate RG for beta-galactosidase and a fluorescence microplate-reader were used.
2. Running a natural selection-type evolution machine. (1) Theoretical studies: It is shown that the estimation of selective value landscape on the base sequence space is important. Selective value distribution in mutants population determines whether the selection profile is the two-bodies type or a cascading type. In the case of large mutation rate, the selection is a cooperative phenomenon and the "free selective value" determines the selection. (2) Hybrid phases among various wild-type strains of the phage Ff were made and the selection coefficient of them were measured. An additivity hypothesis of the contribution of each gene to the whole selection coefficient was studied. (3) A fd phage population having a randomly mutagenized major coat protein was made and was partially selected by the celistat culture.
3. Development of DNA handling process. PCR can amplify the specific gene directly from carrier-cells (or probably from a single carrier-cell) in culture medium.
4. Development of a ISFET micro pH senser. By coating the gate surface with polymers we made a difference-type pH senser. We measured time-cources of inorganic pyrophosphatase catalysis in several tens mul solution. An autocatalytic mechanism by Ca ion was elucidated.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Husimi,Y.: "Confinuous Culture of Bacteriophage Q-beta using a Cellstat with a Bubble Wall-growth Scraper" Reu.Sci.Instrum.58. 1109-1111 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Biebricher,C.K.: "Modeling Studies of RNA Replication and Viral Infection" Proc. of 2nd Int. Workshop on Modeling of Chemical Reactions. 17-38 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y.: "Selection of Single-Stranded DNA Phage in Cellstat Culture" Proc of 19th Yamada Canference on Ordering and Organization in Ionic Solutions. 19. 165-175 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "進化とエントロピ-" 数理科学. 294. 34-39 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "塩基配列空間上の淘汰値の地形と自由淘汰値概念" Viua Origino. 16. 136-141 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishigaki,K.: "Bacterial Bioassay of Microgram to Subnanogram Quantities of Glucose" Biotechnol. and Appl. Biochem.10. 428-434 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y.: "Selection and Evolution of Bacteriophages in Cellstat" Adv.in Biophys.25. 1-43 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "分子進化" 電子情報通信学会誌(印刷中). 73. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakurai,T.: "A Kinetic Study of Inorganic Pyrophosphatase Catalysis Using ISFET Micro pH Senser" Anal Biochem(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtsuka,H.: "A Cloning Machine of Bacteriophage Ff" Rev.Sci.Instrum.(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y.: "Relationship between Distribution Function of Selective Value in Mutants Population and Population Changeover Profile in Continvous Culture" J.Theoret.Biol(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "『R&Dコンピュ-ティング要覧』の「進化モデル」の項" サイエンスフォ-ラム, 608 3 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 品川嘉也: "『バイオ素子開発と設計』の「情報素子としてのDNA、RNA」の項" ミマツデ-タシステム, 422 15 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "生命工学シリ-ズ『生命分子工学』の「人工的自己増殖系」の項(予定)" 裳華房, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 伏見譲: "講座「進化」第6巻『高分子レベルでの進化』の「進化論的分子工学」の項(予定)" 東京大学出版会, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y. and Keweloh,H.-C.: "Continuous Culture of Bacteriophage Q-beta using a Cellstat with a Buble Wall-growth Scraper" Rev.Sci.Instrum.58. 1109-1111 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Biebricher,C.K., Eigen,M., Gardiner,W., Husimi,Y., Keweloh,H.-C. and Obst,A.: "Modeling Studies of RNA Replication and Viral Infection" Proc. of 2nd Int. Workshop on Modeling of Chem. Reactions, pp.17-38 (Springer, 1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y., Nishigaki,K., Kihara,H., and Kinoshita,Y.: "Selection of Single-Stranded DNA Phage in Cellstat Culture" Proc. of 19th Yamada Conf. on Ordering and Organization in Ionic Solutions, pp.165-175 (World Sci.Pub., Singapore, 1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi, Y.: "Evolution and Entropy" Math. Sci., No.294 34-39 (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi, Y.: "Selective Value Landscape on the Base Sequence Space and the Concept of Free Selective Value" Viva Origino, 16, 136-141 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Nishigaki,K., Yamada,T., Watanabe,T., Husimi,Y., and Tanaka,T.: "Bacterial Bioassay of Microgram to Subnanogram Quantities of Glucose" Biotechnol. and Appl.Biochem. 10, 428-434 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi, Y.: "Selection and Evolution of Bacteriophages in Cellstat" Adv. in Biophys., 25, 1-43 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakurai,T., Ohtsuka,H., Kondo,S. and Husimi,Y.: "A Kinetic Study of Inorganic Pyrophosphatase Catalysis Using ISFET Micro pH Senser"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ohtsuka,H., Yoshino,K., Husimi,Y., and Yoshimura, S.: "A Cloning Machine for Bacteriophage Ff"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y. and Amada,K.: "Relationship between Distribution Function of Selective Value in Mutants Population and Population Changeover Profile in Continuous Culture"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Husimi,Y.: "Selection and Evolution of Bacteriophages in Cellstat" Adv.in Biophys.25. 1-43 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 伏見譲: "分子進化" 電子情報通信学会誌. 73. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakurai,T.: "A Kinetic Study of Inorganic Pyrophosphatase Catalysis Using ISFET Micro pH Senser" Anal.Biochem.

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohtsuka,H.: "A Cloning Machine of Bacteriophage Ff" Rev.Sci.Instram.

    • Related Report
      1989 Annual Research Report
  • [Publications] Husimi,Y.: "Relationship between Distribution Function of Selective Value in Mutants Population and Population Changeover Profile in Continuous Caltere" J.Theoret.Biol.

    • Related Report
      1989 Annual Research Report
  • [Publications] 品川嘉也: "バイオ素子開発と設計" ミマツデ-タシステム, (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 伏見譲: "講座「進化」第6巻『高分子レベルでの進化』" 東京大学出版会, (1992)

    • Related Report
      1989 Annual Research Report
  • [Publications] 伏見譲: Viva Origino. 16. 136-141 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Husimi,Y.;etal: Proc.of 19th Yamada Conference. 19. 165-175 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nishigaki,K.;etal: Biotechnol.and Appl.Biochem.10. 428-434 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Husimi,Y.: Adv.in Biophys.25. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 伏見譲 他: "『R&Dコンピューティング要覧』の「進化モデル」の項" サイエンスフォーラム, 259-261 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 伏見譲: "『生命分子工学』の「人工的自己増殖系」の項" 裳華房, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Husimi,Y.; Keweloh,H.-C.: Rev. Sci. Instrum.58. 1109-1111 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Gardiner,W.C.; Eigen,M.; Biebricher,C.K.; Husimi,Y.; Keweloh,H.-C.;Obst,A: Proc of 2nd Int. Workshop on Modeling of Chemical Reactions (Heidelberg, 1986). 2. 1-23 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Husimi,Y.; Nishigaki,K.; Kihara,H.; Kinoshita,Y.: Proc. of 19th Yamada Conterence on Ordering and Organization in Ionic Solutions. 19. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Husimi,Y.: Advances in Biophysics. 24. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 伏見 譲: 数理科学. 294. 34-39 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Husimi, Y.: Viva Origino. 16. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 伏見譲,木原拡 他: "R&Dコンピューティング要覧" サイエンスフォーラム, (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 伏見譲 他: "高機能システム素子としてのバイオ素子開発" ミマツデータシステム, (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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