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Molecular bases of sympathetic innervation and developmental changes in ion channels

Research Project

Project/Area Number 62430025
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionChiba University

Principal Investigator

HASEGAWA Shuji (1989)  School of Medicine, Chiba Univ., Associate Prof, 医学部, 助教授 (20009640)

萩原 彌四郎 (1987-1988)  千葉大学, 医学部, 教授 (10009079)

Co-Investigator(Kenkyū-buntansha) GONOI Tohru  Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba Univ., Resea, 真核微生物研究センター, 助手 (30134365)
KUROMI Hiroshi  School of Medicine, Chiba Univ., Research Associate, 医学部, 助手 (30009633)
長谷川 修司  千葉大学, 医学部, 助教授 (20009640)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥19,000,000 (Direct Cost: ¥19,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1987: ¥11,000,000 (Direct Cost: ¥11,000,000)
Keywordssympathetic innervation / smooth muscle / development / skeletal muscle / ion channels / adhesion / chick / mouse / Geographutoxin / 骨格筋
Research Abstract

(1) SYMPATHETIC INNERVATION-MEDIATING PROTEIN Sympathetic nerve fibirs densely innervate expansor secundariorum muscle, but not skeletal muscle. In cilture sympathetic nerve fibers densely distributed on the substrate from expansor secundariorum but avoided distributing on the substrate from skeletal muscle. The protein which caused the dense distribution of sympathetic fibers as the substrate has been purified from heart cell conditioned medium. The most purified fraction showed single band with apparent molecular weight of 370,000 daltons on SDS-PAGE under non-reducing and reducing conditions. Antiserum was raised against the factor in heart cell conditioned medium. Subcutaneous injection of the antiserum into the chick inhibited the regeneration of adrenergic fibers following 6-hydroxydopamine-induced axotomy in peripheral tissues (heart, spleen, kidney and blood vessel). The results indicate that the protein may function in the process of reinnervation of lesioned sympathetic fiber … More s on target tissues in the chick.
(2) DEVELOPMENTAL CHANGES OF VOLTAGE-SENSITIVE ION CHANNELS The whole-cell voltage-clamp technique was used to examine developmental changes in different types of Ba^<2+> currents through Ca^<2+> channels, Na^+ currents and inward rectifier K^+ currents in single fibres isolated from mouse flexor digitorum brevis muscle. The muscle fibres isolated on day 1 post-natal (P1) showed transient, I(Ca,T), and sustained currents, I(Ca,S). I(Ca,T) became undetectable by P17 and I(Ca,S) increased four-fold in the first 30 days. Denervation did not prevent the disappearance of I(Ca,T). Geographutoxin II(GTX) distinguished GTX-sensitive, I(Na,s), and GTX-resistant, I(Na,r) currents. I(Na,r) became undetectable by P16. I(Na,s) increased 15-fold in the first 20 days. In denervated muscle fibres I(Na,r) was increased above the level of normal fibers. Inwardly rectifying current was split into three components, I(steady-state), I(rise) and I(decay). I(decay) was observed throughout post-natal development. I(steady-state) and I(rise) became apparent after P12. Denervation depressed the increase of I(steady-state) and I(rise). We conclude that different types of ion channel currents in the muscle fibres undergo dramatic changes in their numbers as well as their properties by a few weeks after birth. Some changes are under the control of innervation, but others occur independent of innervation. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Gonoi,T: "The Conus toxin Geographutoxin II distinguishes two functional sodium channel subtypes in rat musclecells developing in vitro." Journal of Neuroscience. 7. 1729-1731 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi,T: "Post-natal disappearance of transient calcium channels in mouse skeletal muscle: Effects of denervation and culture." Journal of Physiology. 401. 617-637 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi,T: "Geographutoxin-sensitive and insensitive sodium currents in mouse skeletal muscle developing in situ." Journal of Physiology. 414. 159-177 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi,T: "Post-natal induction and neural regulation of inward rectifiers in mouse skeletal muscle." Journal of Physiology(submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kuromi,H: "Sympathetic innervation-mediating protein." Neuroscience Research(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Luo,J-J: "Chick sympathetic neurons in culture respond differently to NGF and medium conditioned by lectin-activated splenic cells." Japanese Journal of Pharmacology. 52. 337P-337 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi, T. et al: "The Conus toxin Geographutoxin II distinguishes two functional sodium channel subtypes in rat musclecells developing in vitro." Journal of Neuroscience. 7. 1729-1731 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi, T. et al: "Post-natal disappearance of transient calcium channels in mouse skeletal muscle: Effects of denervation and culture." Journal of Physiology 401:617-637 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi, T. et al: "Geographutoxin-sensitive and insensitive sodium currents in mouse skeletal muscle developing in situ." Journal of Physiology 414:159-177 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi, T. et al: "Post-natal induction and neural regulation of inward rectifiers in mouse skeletal muscle." Journal of Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kuromi, H: "Sympathetic innervation-mediating protein." Neuroscience Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Luo, J-J. et al: "Chick sympathetic neurons in culture respond differently to NGF and medium conditioned by lectinactivated splenic cells." Japanese Journal of Pharmacology 52:337P (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Gonoi,T.: "Geographutoxinーsensitive and insensitive sodium currents in mouse skeletal muscle developing in situ." Journal of Physiology. 414. 159-177 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Gonoi,T.: "Postーnatal induction and neural regulation of inward rectifiers in mouse skeletal muscle." Journal of Physiology(submitted).

    • Related Report
      1989 Annual Research Report
  • [Publications] Kuromi,H.: "Sympathetic innervationーmediating protein." Neuroscience Research(in press).

    • Related Report
      1989 Annual Research Report
  • [Publications] Luo,JーJ: "Chick sympathetic neurons in culture respond differently to NGF and medium conditioned by lectinーactivated splenic cells." Japanese Journal of Pharmacology. 52. 337P (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tohru Gonol;et al.: Jourrial of Neuroscience. 7. 1728-1731 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Gonoi,T.;Hasegawa,S.: Journal of Physiology(London). 401. 617-637 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Gonoi,T.;Hagihara,Y.;et al.: Journal of Physiology(London). (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒見坦,長谷川修司,萩原彌四郎: 代謝. 24. 999-1006 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hiroshi,Kuromi: A.P.J.Pharmacol.2. 195-202 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒見坦: 日薬理誌. 90. 73-81 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tohru Gonoi et al.: Journal of Neuroscience. 7. 1728-1731 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Gonoi, T. Hasegawa, S.: Journal of Physiology. 401. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 黒見坦,長谷川修司,萩原彌四郎: 代謝. 24. 999-1006 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hiroshi Kuromi: A.P.J.Pharmacol.2. 195-202 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 黒見坦: 日薬理誌. 90. 73-81 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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