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Studies on the mutation of mitochondrial DNA in salmonids as a genetic tag for stock management.

Research Project

Project/Area Number 62440016
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

NUMACHI Ken-ichi  Ocean Res. Inst., Univ. of Tokyo Professor, 海洋研究所, 教授 (30013569)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Takanori  Ocean Res. Inst., Univ. of Tokyo Research Associate, 海洋研究所, 助手 (70205467)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1987: ¥7,900,000 (Direct Cost: ¥7,900,000)
Keywordsmitochondrial DNA / salmonids / stock management / restriction fragmental patterns / mtDNA / 切断型 / 集団遺伝学
Research Abstract

Rapid and simple purification of mt DNA in fish species was established by applying SDS-NaCl precipitation instead of DNase treatment of mitochondria pellets, and by adopting low temperature below 20゚ C throughout all the treatments including SDS and RNase treatment. In this procedure, yields of ccDNA were much increased (X 2- 400) compared with the method used so far, and crude mt DNA from 50-60 specimens were prepared within a day. Furthermore, a improved ultrracentrifugal procedure that can separate a larger amount of mtDNA (200mug or more) within 3 hr was developed.
Applying this purification method, 106 specimens of chum salmon from 5 rivers and 86 specimens of 5 natural and artificial sustained populations were analyzed by 10 kinds of 6 base-pair restriction endonuclease. Totally 13 types of mutations were demonstrated in the fragmental patterns produced by digestion of 5 kinds of enzymes, and 9 clonal genotypes of mtDNA were posturated. River populations were consisted of common … More types ( II and III) and some river specific types. The base pair substitutions (delta, Nei and Li) among the clonal genotypes were 0.2-1 %.
In the masu salmon, 22 clonal genotypes in combination of 20 types of fragmental patterns in 7 kinds of enzymes were demonstrated. The base-pair substitutions (delta) among the clonal genotypes were 0.2-2.85 %, and the nucleotide diversity (pi, Nei and Li ) among populations were very high, 0.893-1.531. Each population has their own specific genotypes abundantly. These genotypes were suggested as a useful genetic tags to study population. The Formosan masu salmon consisted of 29 specimens showed only one clonal genotypes, which showed much resemblance with the types in the masu distributed in the northern part of Japan. The base pair substitutions between them were 0.2-1.59 %. We concluded that the Formosan salmon originated from the masu salmon in the Sea of Japan through the Tsushima channel at 100-800 thousands years ago, and the unusual homogeneity observed in the Formosan salmon should be caused by recent contracted stage of population. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ken-ichi Numachi,Takanori Kobayashi,Shiro Wada and Etsuko Shimura: "Mitochondrial DNA variation and its use for cetacean population study." IWC Workshop Document,SC/89/20. 12pp (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shiro Wada,Takanori Kobayashi and Ken-ichi Numachi: "Genetic variability and differentiation of mitochondrial DNA in Minke Whale." IWC Workshop Document,SC/41/SHMi 19. 14pp (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ken-ichi Numachi, Takanori Kobayashi, Kun-Hsing Chang and Yao-Sung Lin: "Genetic identification of the Formosan landlocked salmon, Oncorynchus masou formusanus by restriction anslysis of mito-chondrial DNA." Academia Sinica(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ken-ichi Numachi, Takanori Kobayashi, Shiro Wada, and Etsuko Shimura: "Mitochondrial DNA variation and its use for cetacean population study." IWC Workshop. Document SC/89/20. 12 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Shiro Wada, Takanori Kobayashi, and Ken-ichi Numachi: "Genetic variability and differentiation of mitochondrial DNA in Minke Whale" IWC Workshop, Document SC/41/SHMi 19, 14pp. (1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ken-ichi Numachi, Takanori Kobayashi, Kun-Hsing Chang and Yao-Sung Lin: "Genetic identification and differentiation of the Formosan landlocked salmon, Onorynchus masou formosanus, by restriction analysis of mitochondrial DNA." Academia Sinica, (1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Numachi,T.Kobayashi,K.Chang,and Y.Lie: "Genetic identification of differentiation of Formosan land locked salmon,Oncorhynchus masow for mosanus by restriction analysis of mitochondrial DNA"

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Wada,T.Kobayashi,and K.Numachi: "Genetic variability and differentiation of mitochondrial DNA in Minlce Whales" IWC.Genetic workshop,Document SC/89/GEN20. 1299 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Numachi,T.Kobayashi,S.Wada,and E.Shimura: "Mitochondrial DNA Variation and its use for cetacean population study" IWC Genetic workshop,Document SC/41/SHMi19. 1499 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Numachi;T.Kobayashi: Biochemical genetics.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Numachi;T.Kobayashi: Genetics.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Numachi; T. Kobayashi: Biochemical Genetics.

    • Related Report
      1987 Annual Research Report
  • [Publications] K.Numachi; T. Kobayashi: Genetics.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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