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Immunopotentiatroy Effects of TLA and Obioactin on the Acquired Immunodeficiency Syndroms (AIDS) in animals

Research Project

Project/Area Number 62440020
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionObihiro University

Principal Investigator

SUZUKI Naoyoshi  Obihiro Univ., Veterinary. Physiology, Prof., 畜産学部・家畜生理学講座, 教授 (10003071)

Co-Investigator(Kenkyū-buntansha) SATO Motoyoshi  Obihiro Univ., Vet. Clinical Radiology, Assoc. Prof., 畜産学部・獣医放射線学講座, 助教授 (50003140)
MIKAMI Masayuki  Obihiro Univ., Bio. Resource Sciences, Assoc. Prof., 畜産学部・生物資源利用学講座, 助教授 (40003107)
SAITO Atushi  Obihiro Univ., Vet. Physiology, Assoc. Prof., 畜産学部・家畜生理学講座, 助教授 (10002263)
MIURA Hiroyuki  Obihiro Univ., Bio. Resource Sciences, Prof., 畜産学部・生物資源利用学講座, 教授 (90003079)
HIROSE Tsuneo  Obihiro Univ., Vet. Clinical Radiology, Prof., 畜産学部・獣医放射線学講座, 教授 (60003076)
Project Period (FY) 1987 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥27,100,000 (Direct Cost: ¥27,100,000)
Fiscal Year 1990: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1987: ¥14,500,000 (Direct Cost: ¥14,500,000)
KeywordsToxoplasma lysate antigen (TLA) / immunopotentiator / Protozoan parasites / Natural killer cells / Macrophages / LAK cells / FeLN resistance / Tumor inhibition / トキソプラズマ溶解抗原 / オビオアクチオ / 免疫賦活・調整物資 / Killer細胞 / 単核細胞誘導 / トキソプラズマ可溶性成分 / 免疫賦活物質 / BRM / -IFN誘導 / NK細胞活性誘導 / T-細胞誘導 / Meth A腫瘍 / オビオアクチン / 免疫調整物質
Research Abstract

Toxoplasma gondii has been reported to stimulate non-specific resistance to rodent malaria and babesia diseases. Mice that were treated systemically with Toxoplasma lysate antigen (TLA) were highly resistant to infection with Plasmodium berghei and Babesia rodhaini. Consequently, studies of the behavior of lymphocytes and natural killer (NK) cells and lymphokines activated killer (LAK) cells from TLA sensitized animals may clarify the factors responsible for resistance to infection with protozoan parasites and with tumors.
When adult BALB/C mice were sensitized with two intramuscular injection of TLA at 2 week interval, the numbers of Sig(+), Thy-1(+), Lyt-2, 2(+), and asialo GM1(+) cells in the spleen, liver and peripheral blood increased by 2 to 4 times over those found in unsensitized mice of the same age. When TLA-sensitized and unsensitized mice were inoculated with Babesia, 4 of 10(40%) of the TLA-sensitized mice survived infection, while none of the unsensitized control mice live … More d longer than 14 days after infection. By contrast sensitization of nude mice with TLA has no effect on survival, and mice did not live more than 12 days. The number of thymic Thy-1, 2(+) cells decreased in TLA-sensitized and unsensitized BALA/c mice by almost 80% within 10 days after infection. During the same time, the numbers of B cells, T cells, and NK cells increased in the spleen, liver and peripheral blood of both sensitized and unsensitized mice. Especially notable were increases in the numbers of Lyt-2,2 cells in the spleen and blood and increases in numbers of NK cells in the spleen, liver and blood in both TLA-sensitized and unsensitized mice. When spleen cells from TLA-sensitized and unsensitized mice were cultured in the presence or absence of TLA for 6 days, assays for cytotoxicity using NK-insensitive P-815 target cells and NK-sensitive YAC-1 target cells demonstrated higher rates of cytotoxicity in cultures of TLA-sensitized spleen cells. The present results showed clearly that addition of TLA to cultures of TLA-sensitized spleen cells stimulates production of killer cells, such as lymphokine activated killer (LAK) cells and NK-like LAK and/or NK cells. Consequently, resistance of TLA sensitized mice to protozoan infection may be mediated not only by increases in the numbers of Thy-1, 2(+) and asialo GM1(+) cells, but also by quantitative and qualitative variations in these cell types. Less

Report

(5 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Suzuki,N.et al.: "Modulator effect of Toxoplasma lysate antigen in mice experimentally infected with Plasmodium berghei" Zbl.Bakt.Hyg.A.264. 422-434 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai,H.et al.: "Long term in vitro suspension culture of OOToxoplasmaWWーPP and Hele Cells" Zbl.Bakt.Hyg.A.264. 468-477 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito,A.et al.: "Inhibitory effect of Toxoplasma lysate antigen on the multiplication of transplanted tumor in mice" Zbl.Bakt.Hyg.A.271. 114-126 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Omata,Y.et al.: "Isospora felis:Possible evidence for transmission of parasites from chronically infected mother cats to kittens" Jpn.J.Vet.Sci.52. 665-666 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato,M.et al.: "protection against Babesia infection beagles immunized with Toxoplasma lysate antigen" Jpn.J.Vet.Sci.52. 155-158 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yang,M.et al.: "Effects of Toxoplasma lysate antigen on feline cytotoxicity against FeLV positive lymphoma cells" Jpn.J.Vet.Sci.52. 735-742 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 鈴木 直義: "実験動物のバベシア病 (獣医住血微生物病)" 近代出版社、東京, 16-45 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 鈴木 直義: "Obioactin,生体応答修正・修飾物質 中の天然免疫調整物質(獣医学)" 近代出版社、東京, 76-90 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato, M. et al.: "Protection against Babesia infection in beagles immunized with TLA" Japanese Journal of Veterinary Science (Jpn. J. Vet. Sci.). 52. 155-158 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Omata, Y. et al.: "Isospora felis : Possible evidence for transmission of parasites from chronically infected mother cats to kittens" Jpn. J. Vet. Sci.52. 665-666 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Omata, Y. et al.: "Experimental feline toxoplasmosis : Humoral responses of cats inoculated orally with Toxoplasma gondii cysts and oocysts" Jpn. J. Vet. Sci.52. 865-867 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yang, M. et al.: "Effects of Toxoplasma lysate antigen (TLA) on feline cytotoxicity against FeLV positive lymphoma cells" Jpn. J. Vet. Sci.52. 735-742 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Suzuki, N. et al.: "Biological activity of Obiopeptide-1, a synthetic peptide derived from the native immune regulator Obioactin" Jpn. J. Vet. Sci.52. 907-914 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Igarashi, I. et al.: "Changes of lymphocyte subpopulations and natural killer cells in mice sensitized with TLA before and after Babesia infection" Jpn. J. Vet. Sci.52. 969-977 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito, A. et al.: "Inhibitory effect of Toxoplasma lysate antigen on the multiplication of transplanted tumor in mice" Zbl. Bakt. Hyg. A.271. 114-126 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakurai, H. et al.: "Long term in vitro suspension culture of Toxoplasma and Hela Cells" Zbl. Bakt. Hyg. A.264. 468-477 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito, A. et al.: "Correlation between release of reactive oxygen intermediates and inhibition of toxoplasma multiplication in mouse peritoneal and alveolar macrophages and kidney cells in vitro incubation with Obioactin, TLA, MDP or Lonomycine A" Zbl. Bakt. Hyg. A.264. 446-454 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Suzuki,N.et al.: "Modulator effect of Toxoplasma lysate antigen in mice experimentally infected with <Plasmodium>___ー <berghei>___ー" Zbl.Bakt.Hyg.A.264. 422-434 (1987)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sakurai,H.et al.: "Long term in vitro suspension culture of <Toxoplasma>___ー and Hela Cells" Zbl.Bakt.Hyg.A.264. 468-477 (1987)

    • Related Report
      1990 Annual Research Report
  • [Publications] Saito,A.et al.: "Inhibitory effect of Toxoplasma lysate antigen on the multiplication of transplanted tumor in mice" Zbl.Bakt.Hyg.A.271. 114-126 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Omata,Y.et al.: "Isospora felis:Possible evidence for transmission of parasites from chronically infected mother cats to kittens" Jpn.J.Vet.Sci.52. 665-666 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sato,M.et al.: "Protection against Babesia infection in beagles immunized with Toxoplasma lysate antigen" Jpn.J.Vet.Sci.52. 155-158 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yang,M.et al.: "Effects of Toxoplasma lysate antigen on feline cytotoxicity against FeLV positive lymphoma cells" Jpn.J.Vet.Sci.52. 735-742 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 鈴木 直義: "実験動物のバベシア病(獣医住血微生物病)" 近代出版社、東京, (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 鈴木 直義: "Obioactin,生体応答修正・修飾物質中の天然免疫調整物質(獣医学)" 近代出版社、東京, (1987)

    • Related Report
      1990 Annual Research Report
  • [Publications] 斉藤篤志ら: "トキソプラズマ溶解抗原(TLA)によって誘導される細胞障害性細胞の性質に関する基礎的検討" Biotherapy. 3. 329-333 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Saito,A.et al.: "Inhibitory effect of Toxoplasma lysate antigen on the multiplication of transplanted tumor in mice." Zbl.Bakt.Hyg.l.A. 271. 114-126 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 鈴木直義ら: "マウス・マクロファ-ジの培養と細胞活性の測定" 家畜生化学研報. 23. 33-44 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,M.et al.: "Protection against Babesia infection in beagles immunized with Toxoplasma lysate antigen." Jpn.J.Vet.Sci. 52. 155-158 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoda,T.et al.: "Changes in immune serum of Toxoplasma gondii following administration of T.gondii lysate antigen." Res.Bull.Obihiro Univ.,Series. 16. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 鈴木直義 他: Biotherapy. 2. 96-98 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 斎藤篤志 他: Biotherapy. 3. 329-333 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Saito,A;et al: Zbl. Baht. Hgg. I. Abt. Orig,A.266. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sakurai,H.;Terada,S.;Igarashi,I.; Saito,A.; Suzuki,N.: Zentral Blatt fuer Bakteriologie, Parasitolqgie & Hygiene(Zbl.Bakt.Hyg.) Original A. 265. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyahara,K; Igarashi,M.; Inomata; Sakurai,H.;Saito,A; Hirose,T.; Suzuki,N.: Zbl. Bakt. Hyg. Original A. 265. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Saito,A.;Miyahara,K.;Sakurai,H.;Hirose,T.;Suzuki,N.: Zbl. Bakt. Hyg. Original A. 265. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] N. Suzuki et al: Zbl. Bakt. Hyg. Original A. 264. 422-434 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Satoh,M. et al: Zbl. Bakt. Hyg. Original a. 264. 435-445 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 伊沢久夫,清水悠紀臣 編 鈴木直義: "獣医学 1986:オビオアクチンーOBIOACTIN-生体応答修飾物質(BRM) 中の天然免疫調整物質として" 近代出版, 238 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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