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Clarification of Biological Meaning of Gap-Junctional Intercellular Communication by Using Monoclonal Antibodies

Research Project

Project/Area Number 62440022
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionHiroshima University

Principal Investigator

KANNO Yoshinobu  Hiroshima University school of Dentistry, Professor, 歯学部, 教授 (00034158)

Co-Investigator(Kenkyū-buntansha) HIRONO Chikara  Hiroshima University school of Dentistry, Research Associate, 歯学部, 助手 (10199135)
SHIBA Yoshiki  Hiroshima University school of Dentistry, Associate Professor, 歯学部, 助教授 (90110452)
佐々木 康人  広島大学, 歯学部, 助手 (50136090)
Project Period (FY) 1987 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥7,300,000 (Direct Cost: ¥7,300,000)
KeywordsGap junctions / Intercellular communication / Monoclonal antibody / Rat liver / Rat salivary gland / Cultured cells / Cell proliferation / Secretion / 認識部位 / 発癌 / モノクローナル抗体 / 唾液腺細胞 / Cキナーゼ / 細胞分化 / ギャップ結合蛋白質 / ラット肝細胞 / ラット唾液腺細胞 / 細胞間結合 / 細胞間チャネル
Research Abstract

In this project, we investigated the biological meaning of gap-junctional intercellular communication, by using monoclonal antibodies against gap junction proteins. Gap junction protein (27KDa) was purified from sodium hydrochoride-insoluble fraction of rat liver homogenate, and a monoclonal antibody against purified gap junction protein was obtained according to the conventional procedure. Monoclonal antibody (14-84Al) recognized 27 KDa gap junction protein in the crude membrane fraction from rat liver, and stained the interecellular membrane of hepatocytes as spotted appearance. To clarify the biological meaning of gap junctional communication, its relationship to the cell proliferation and the secretory activity was investigated. The gap junction proteins in the isolated hepatocytes rapidly disappeared after culturing in vitro. These results suggest that gap-junctional intercellular communication is not required in the proliferating hepatocytes. The gap-junctional communication in cultured 3T3-L1 cells also exerted suppressive action on the proliferation.
The gap-junctional communication in the salivary gland acinar cells was related to the activity of the acinar cells to secrete the salivary juice.
The control of the expression of gap junction protein was also investigated by using monoclonal antibodies. Cultured cells derived from the liver was not stained with 14-84Al monoclonal antibody, but these cell lines exhibited well-developed dye-coupling, suggesting the modulation of the expression of gap junction proteins by in vitro cultures. The gap junctions of acinar cells in rat salivary glands were composed of 27 KDa gap junction proteins, and required the innervation of autonomic nerve system for its maintenance. Further studies using monoclonal antibodies against an other gap junctional proteins are necessary to clarify the control mechanism of the expression of gap junction proteins.

Report

(5 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] 菅野 義信: "唾液腺腺房細胞のギャップ結合の役割を考える" 日本唾液腺学会誌. 31. 11-23 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshiki,Shiba: "Inhibition of gapーjunctional intercellular communication and enhanced bindin of fibronectinーcoated latex beads by stimulation of DNA synthesis in quiescent 3T3ーLl cells" Jounal of Cellular Physiology. 145. 268-273 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanno,Yoshinobu: "The regulation and maintenance of the gap junctional intercellular communication in submandibular glands of rat" First APOCB Congess abstracts:94. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hirono,Chikara: "Monoclonal Antibodies against gap junction protein from rat liver" The Japanese Journal of Physiology. 40. 15-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐々木 康人: "分泌刺激後のラット顎下腺ギャップ結合機能の長時的追跡" 日本生理誌. 52. 117-117 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanno,Yoshinobu: "Reduction of intercellular communication in submandibular gland acinar cells through calcium influx and protein kinase C by acetylcholine and adrenaline" Elsevier Science Publishers, 99-102 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅野 義信: "電気的結合:新生理学大系,第7巻発生・分化の生理学" 医学書院, 180-188 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanno, Yoshinobu: "Effects of denervation on cell-to-cell communication between acinar cells of rat submandibular salivary gland." Archs. Oral Biol.32(1). 43-46 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki, Yasuto: "Suppression of intercellular communication in acinar cells from rat submandibular gland by cholinergic and adrenergic agonists." Jpn. J. Physiol.38(4). 531-543 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanno, Yoshinobu: "Autonomic control of intercellular communication in salivary glands." Proceedings of the Finnish Dental Society. 85(4-5). 301-305 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shiba, Yoshiki: "Close relationship between modulation of serum-induced stimulation of DNA synthesis and changes in gap-junctional intercellular communication in quiescent 3T3-L1 cells caused by cyclic AMP and the tumor-promoting phorbol ester TPA." Exp. Cell Res.185(2). 535-540 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasaki, Yasuto: "Herbimycin A suppresses the reduction of gap-junctional intercellular communication induced by tumor-promoting phorbol ester in 3T3-L1 cells." Jpn. J. Cancer Res.80(9). 855-860 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shiba, Yoshiki: "Inhibition of gap-junctional intercellular communication and enhanced binding of fibronectin-coated latex beads by stimulation of DNA synthesis in quiescent 3T3-L1 cells." J. Cell. Physiol.145(5). 268-273 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kanno, Yoshinobu: "Reduction of intercellular communication in submandibular gland acinar cells through calcium influx and protein kinase C by acetylcholine and adrenaline." Bioinformatics. Ed by O. Hatase & J. H. Wang. Elsevier Science Publishers. 99-102 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅野 義信: "唾液腺腺房細胞のギャップ結合の役割を考える" 日本唾液腺学会誌. 31. 11-23 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shiba,Yoshiki: "Inhibition of gapーjunctional intercellular communication and enhanced binding of fibronectinーcoated latex beads by stimulation of DNA synthesis in quiescent 3T3ーLl cells" Jounal of Cellular Physiology. 145. 268-273 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "The regulation and maintenance of the gap junctional intercellular communication in submandibular glands of rat" First APOCB Congress abstracts:94. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirono,Chikara: "Monoclonal Antibodies against gap junction protein from rat liver" The Japanese Journal of Physiology. 40. 15-15 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐々木 康人: "分泌刺激後のラットの顎下腺ギャップ結合機能の長時的追跡" 日本生理誌. 52. 117-117 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "Reduction of intercellular communication in submandibular gland acinar cells through calcium influx and protein kinase C by acetylcholine and adrenaline" Elsevier Science Publishers, 99-102 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅野 義信: "電気的結合:新生理学大系,第7巻発生・分化の生理学" 医学書院, 180-188 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅野義信: "ギャップ結合のバイオロジ-" 生体の科学. 40. 650-657 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shiba,Yoshiki: "Close ralationship between modulation of serum-induced stimulation of DNA synthesis and changes in gap-junctional intercellular communication in quienscent 3T3-L1 cells caused by cyclic AMP and the tumor-promoting phorbor ester TPA." Experimental Cell Research. 185. 535-540 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "Inhibition of intercellular communication in acinar cells from rat submandibular gland by acetylcholine and adrenaline." Proceedings of the International Union of Physiological Sciences. 17. 154-154 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "The autonomic control of intercellular communication in salivary gland." Proceedings of the Finnish Dental Society. 85Suppl 1. 30-30 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sasaki,Yasuto: "Decrease in intercellular communication between acinar cells of rat submandibular gland after salivary secretion." The Japanese Journal of Physiology. 39. S24-S24 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sasaki,Yasuto: "Herbimycin A suppresses the reduction of gap-junctional intercellular communication induced by tumor-promoting phorbor ester in 3T3-L1 cells." Japnaese Journal of Cancer Research. 80. 855-860 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "Bioinformatics" Elsevier Science Publishers, 444 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] sasaki,yasuto: The Japanese Journal of physiology. 38. 531-543 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] sasaki,yasuto: Journal of The physiological society of Japan. 50. 379-379 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanno,Yoshinobu: Fourth International Congress of cell Biology Abstracts. 234-234 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasaki,Yasuto: Cell Structure and Function. 13. 629-629 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasaki,Yasuto: Journal of Dental Research. 68. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanno,yoshinobu: Neuroscience Research,Supplement. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanno,Yoshinobu: "Proceedings of International symposium on Information Transduction and processing in Biological systems" Elsevier science publishers, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kanno, Yoshinobu: Archives of oral Biology. 32. 43-46 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sasaki, Yasuto: The Japanese Journal of Physiology. 38. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sasaki, Yasuto: Journal of The Physiological Society of Japan. 49. 336-336 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅野義信: 組織培養. 13. 142-145 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅野義信: 蛋白質・核酸・酵素. 32. 1460-1463 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅野義信: 蛋白質・核酸・酵素. 32. 1176-1180 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅野義信: "新生理科学大系5 分泌の生理学" 医学書院, 335 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 菅野義信: "基礎歯科生理学" 医歯薬出版株式会社, 398 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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