• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic Research and Clinical Application of Hematopoietic Growth Factors

Research Project

Project/Area Number 62440049
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionFaculty of Medicine, University of Tokyo

Principal Investigator

YAMAJI Tohru (1990)  Faculty of Medicine, University of Tokyo, Lecturer, 医学部・附属病院, 構師 (30010143)

高久 史磨 (高久 史麿) (1987-1989)  東京大学, 医学部(病)・第三内科, 教授 (40048955)

Co-Investigator(Kenkyū-buntansha) YAMADA Nobuhiro  Faculty of Medicine, University of Tokyo, Assistant Prof., 医学部・附属病院, 助手 (40200729)
KITAMURA Kiyoshi  Faculty of Medicine, University of Tokyo, Lecturer, 医学部・附属病院, 構師 (10186265)
OKABE Tetsuro  Faculty of Medicine, University of Tokyo, Assistant Prof., 医学部・附属病院, 助手 (80169135)
HIRAI Hisamaru  Faculty of Medicine, University of Tokyo, Lecturer, 医学部・附属病院, 構師 (90181130)
TAKAKU Fumimaro  National Medical Center, Director, 医療センター, 病院長 (40048955)
宮川 清  東京大学, 医学部(病)・第三内科, 医員
石川 冬木  東京大学, 医学部(病)・第三内科, 助手 (30184493)
宮園 浩平  東京大学医学部(病), 第三内科, 助手 (90209908)
春日 雅人  東京大学, 医学部, 助手 (50161047)
大沢 仲昭  東京大学, 医学部, 助教授 (90010090)
山田 明  東京大学, 医学部(病), 助手 (70175660)
Project Period (FY) 1987 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥27,200,000 (Direct Cost: ¥27,200,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1987: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsColony-stimulating factor / Neutropenia / Hematopoietic growth factors / Leukemia / Bone marrow transplantation / Aplastic anemia / GーCSF / MーCSF / GMーCSF / インタ-ロイキンー3 / インタ-ロイキンー1 / エリスロポエチン / 造血幹細胞 / コロニー刺激因子
Research Abstract

We have investigated the possibility of clinical use of hematopoietic growth factors in vitro and in vivo. Granurocyte colony-stimulating factor (G-CSF) promotes proliferation and differentiation of granulocytic precursors, release of mature nuetrophils from bone marrow, and a variety of functions of mature neutrophils. Effects of G-CSF on granulocytes enhance the activity of defense for microbiological infection. Thus of G-CSF can be considered to have an important role in the mechanism of defense for infection. In the in vivo analysis, administration of even a small amount of G-CSF (3 mug/kg) increased white blood cells in several hours. G-CSF was effective in accelerating recovery from neutropenia after chemotherapy and bone marrow transplantation. G-CSF decreased the frequency of severe infection in the neutropenic period after chemotherapy and bone marrow transplantation. We also tried to treat patients with aplastic anemia and myelodysplastic syndrome (MDS) with G-CSF. They generally responded to a higher dose of G-CSF. Myeloid cells also increased in their bone marrow. A part of patients with aplasic anemia and MDS responded to G-CSF resulting in an increase of neutrophils. Further, G-CSF was effective in patients with severe infections and chronic neutropenia. We administrated G-CSF and anti-leukemic agents simultaneously to treat refractory acute non-lymphocytic leukemias. Administration of G-CSF and anti-leukemic agents induced a rapid disapearance of blasts. The simultaneous administration was considered to be effective in some cases with refractory acute nonlymphocytic leukemia.

Report

(5 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Yuo,A.,et al: "Recombinant human granulocyte colonyーstimulating factor repairs the abnormalities of neutrophils in patients with myelodysplastic syndromes and chronic myelogenous leukemia" Blood. 70. 404-411 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tojo,A.,et al: "Induction of the receptor for erythropoientin in murine erythroleukemia cells after dimethyl sulfoxide treatment." Cancer Res.48. 1818-1822 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kitamura,T.,et al: "Identification and analysis of human erythropoietin receptors on a factorーdependent cell line,TFー1." Blood. 73. 375-380 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Motoyoshi,K.,et al.: "Serum cholesterolーlowering activity of human monocytic colony stimulating factor." Lancet. 1. 326-327 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hino,M.,et al.: "Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells." J.Biol.Chem.264. 10309-10314 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chiba,S.,et al.: "Characterization and molecular features of the cell surface receptor for human granulocyteーmacrophage colonyーstimulating factor" Leukemia. 4. 29-36 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高久史麿 ほか: "造血機構とその調節因子" 羊土社, 204 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高久史麿 ほか: "白血病の分化誘導療法" 中外医学社, 218 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Shimamura, Y. Kobayashi, A. Yuo, A. Urabe, T. Okabe, Y. Komatsu, S. Itoh, F. Takaku: "Effect of Human Recombinant Granulocyte Colony-Stimulating Factor on Hematopoietic Injury in Mice Induced by 5-Fluorouracil" Blood. 69(1). 353-355 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A. Yuo, S. Kitagawa, T. Okabe, A. Urabe, Y. Komatsu, S. Itoh, F. Takaku: "Recombinant Human Granulocyte Colony-Stimulating Factor Repairs the Abnormalities of Neutrophils in Patients With Myelodysplastic Syndromes and Chromic Myelogenous Leukemia" Blood. 70(2). 404-411 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] A. Tojo, H. Fukamachi, T. Saito, M. Kasuga, F. Takaku: "Induction of the Receptor for Erythropoietin in Murine Erythroleukemia Cells after Dimethyl Sulfoxide Treatment" Cancer Res. 48. 1818-1822 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitamura, A. Tojo, T. Kuwaki S. Chiba, K. Miyazono, A. Urabe, F. Takaku: "Identification and Analysis of Human Erythropoietin Receptors on a Factor-Dependent Cell Line, TF-1" Blood. 73(2). 375-380 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Motoyosih, F. Takaku: "Serum Cholesterol-Lowering Activity of Human Monocytic Colony-Stimulating Factor" The Lancet. August 5. 326-327 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Hino, A. Tojo, K. Miyazono, Y. Miura, S. Chiba, Y. Eto, H. Shibai, F. Takaku: "Characterization of Cellular Receptors for Erythroid Differentiation Factor on Murine Erythroleukemia Cells" J. Biol. Chem.264(17). 10309-10314 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kitamura, T. Tange, T. Terasawa, S. Chiba, T. Kuwaki, K. Miyagawa, Y. Feng-Piao, K. Miyazono, A. Urabe, F. Takaku: "Establishment and Characterization of a Unique Human Cell Line that Proliferates Dependently on GM-CSF, IL-3, or Erythropoietin" J. Cellular Physiol. 140. 323-334 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Chiba, A. Tojo, T. Kitamura, A. Urabe, K. Miyazono, F. Takaku: "Characterization and Molecular Features of the Cell Surface Receptor for Human Granulocyte-Macrophage Colony-Stimulating Factor" Leukemia. 4(1). 29-36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chiba, K. Shibuya, Y. -F. Piao, A. Tojo, N. Sasaki, S. Matsuki, K. Miyagawa, K. Miyazono, F. Takaku: "Identification and Cellular Distribution of Distinct Proteins Forming Human GM-CSF Receptor." Cell Regulation. 1. 327-335 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuo,A.,et al.: "Recombinant human granulocyte colonyーstimulating factor repairs the abnormalities of neutrophils in patients with myelodysplastic syndromes and chronic myelogenous leukemia." Blood. 70. 404-411 (1987)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tojo,A.,et al.: "Induction of the receptor for erythropoietin in murine erythroleukemia cells after dimethyl sulfoxide treatment." Cancer Res.48. 1818-1822 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kitamura,T.,et al.: "Identification and analysis of human erythropoietin receptors on a factorーdependent cell line,TFー1." Blood. 73. 375-380 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Motoyoshi,K.,et al.: "Serum cholesterolーlowering activity of human monocytic colonyーstimulating factor." Lancet. 1. 326-327 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hino,M.,et al.: "Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells." J.Biol.Chem.264. 10309-10314 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chiba,S.,et al.: "Characterization and molecular features of the cell surface receptor for human granulocyteーmacrophage colonyーstimulating factor." Leukemia. 4. 29-36 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高久 史麿ほか: "造血機構とその調節因子" 羊土社, 204 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高久 史麿ほか: "白血病の分化誘導療法" 中外医学社, 218 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Kobayashi,A.Urabe,F.Takaku,et al.: "Treatment of myelodysplastic syndromes with recombinant human granulocyte colony-stimulating factor:A preliminary report" American Journal of Medicine. 86. 178-182 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Hino,K.Miyazono,A.Urabe,F.Takaku: "Effects of recombinant human erythropoietin on hematopoietic progenitors of chronic hemodialysis patients in vitro and in vivo" International Journal of Cell Cloning. 7. 257-262 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Hino,A.Tojo,K.Miyazono,F.Takaku,et al.: "Characterization of cellular receptors for erythroid differentiation factor on murine erythroleukemia cells." Journal of Biological Chemistry. 264. 10309-10314 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitamura,S.Chiba,A.Urabe,F.Takaku,et al.: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF,IL-3,or erythropoietin" Journal of Cellular Physiology. 140. 323-334 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Yuo,S.Kitagawa,K.Miyazono,F.Takaku,et al.: "Recombinant human granulocyte colony-stimulating factor as an activator of human granulocytes." Blood. 73. 375-380 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.ーF.Piao,S.Chiba,K.Miyazono,F.Takaku,et al.: "Binding properties and proliferative effects of human recombinant granulocyte-macrophage colony stimulating factor in primary leukemia and lymphoma" Japanese Journal of Cancer Reseach. 80. 887-894 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kuwaki,F.Takaku et al.: "Characterizetion of human interleukin 3 receptors on a multifactor dependent cell line." Biochemical and Biophysical Research Communication. 161. 16-22 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Shibuya,F.Takaku et al.: "Effects of ubenimex on proliferation and differentiation of human bone marrow cells and leukemic cell lines,and the possible mechanisms of its action." Experimental Hematology Today. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Chiba,F.Takaku et al.: "Characterization and molecular features of the cell surface receptor for human granulocyte-macrophage colony-stimulating factor." Leukemia. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Yuo,F.Takaku et al.: "Tumor necrosis factor as an antivator of neutrophils" Journal of Immunology. 142. 1678-1684 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Kitamura,F.Takaku,et al.: "Identification and anlysis of human erythropoietin receptors on a factor-dependent cell line,TF-1" Blood. 73. 375-380 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akio Urabe 他: International Journal of cell cloning. 6. 179-191 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] H. Uzumaki 他: Biochemical and Biophysical Research Communication. 156. 1026-1032 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Arinobu Tojo 他: Cancer Research. 48. 1818-1822 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] C-J Tsao 他: Journal of Immunology. 140. 89-93 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 朴雲峰 他: 日本血液学会雑誌. 51. 1115-1121 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukio Kobayashi 他: American Journal of Medicine. 86. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Michiya Shimamura 他: Experimental Hematology. 16. 681-685 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toshio Kitamura 他: Acta Haematologica Japonica. 51. 677-685 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Masayuki Hino 他: British Journal of Haematology. 70. 143-147 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] A. Yuo: Blood. 70. 404-411 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Shimamura: Blood. 69. 353-355 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Y. Kobayashi: Jpn. J. Cancer Res.78. 763-766 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] H. Fukamachi: Int. J. Cell Cloning. 5. 209-219 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] J. Nishida: Jpn. J. Exp. Med.56. 321-323 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Kitagawa: Biochem. Biophys. Res. Commun.144. 1143-1146 (1987)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi