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ヒトの遺伝子マップと染色体のDNAセグメントマップの作成

Research Project

Project/Area Number 62440083
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionKeio University

Principal Investigator

清水 信義  慶應義塾大学, 医学部, 教授 (50162706)

Co-Investigator(Kenkyū-buntansha) 工藤 純  慶應義塾大学, 医学部, 助手 (80178003)
Project Period (FY) 1987 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥29,200,000 (Direct Cost: ¥29,200,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1987: ¥9,000,000 (Direct Cost: ¥9,000,000)
Keywords遺伝子マップ / FACS 440セルソ-タ- / 雑種細胞パネル / 染色体ソ-ティング / Calpain subunit / Calpastatin / 水疱性類天疱瘡抗原蛋白遺伝子 / スポットブロットハイブリダイゼ-ション / 雑種細胞 / Adenine nucleotide translocater / 遺伝子マッピング / カルパイン / ATP synthase βサブユニット / プロテインキナ-ゼC / Phosphoribosylpyrophosphate synthetase / 染色体ソーティング / Myeloperoxidase / Interleukin 4 / プロテインキナーゼC / セルソーター / ミエロパーオキシダーゼ / インターロイキン4 / インスリンレセプター / ホスホリボシルピロリン酸シンテターゼ
Research Abstract

我々は染色体ソ-ティングの技術に改良を加え、最近ポリアミン・ジギトニン処理法でヒト培養リンパ球(GM130B)から得た分裂中期染色体を、ヘキスト33258またはヨウ化プロピジウムで染色し、FACS 440セルソ-タ-を用いて合計18画分に分別できるようになった。さらに各染色体画分を直接ニトロセルロ-スフィルタ-に集め、ラベルしたDNAプロ-ブとのスポットブロットハイブリダイゼ-ションを組織的に行う技術も確立した。このようにソ-ティングした染色体と雑種細胞パネルのDNAを併用して、本年度は以下のヒト遺伝子をマッピングした。Calpain subunit1(CAPN1)/第11染色体、同subunit2(CAPN2)/第1染色体、同subunit関連遺伝子(CAPN3)/第15染色体、同subunit4(CAPN4)/第19染色体、Calpastatin(CAST)/第5染色体、水疱性類天疱瘡抗原蛋白遺伝子/第6染色体にマップした。

Report

(4 results)
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ohno,S.: "Four genes for the Calpain family locate on four distinct human chromosomes." Cytogenet.Cell Genet.53. 225-229 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inazawa,J.: "Assignment of the human calpastatin gene (CAST) to chromosome 5 at a region q14ーq22." Cytogenet.Cell Genet.54. 156-158 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Minoshima,S.: "Localization of the human gene for 230ーkDa Bullous Penphigoid autoantigen to the pter region of chromosome 6." Cytogenet.Cell Genet.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Minoshima,S.: "Isolation of Giant Fragments from Flow-Sorted Human Chromosomes." Cytometry. 11. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Neckelmann,N.: "The Human ATP Synthetase β Subunit Gene:Sequence Analysis,Chromosome Assignment and Differential Expression." Genomics. 5. 829-843 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Li,K.: "The Human Muscle Adenine Nucleotide Translocator Gene has Four Exons,is Located on Chromosome 4 and is Developmentally Regulated." J.Biol.Chem.264. 13998-14004 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakai,K.: "Isolation of cDNAs Encoding a Substrate for Protein Kinase C:Nucleotide Sequence and Chromosomal Mapping of the Gene for a Human 80Κ Protein." Genomics. 5. 309-315 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Taira,M.: "Localization of the Human Phosphoribosylpyrophosphate Synthetase Subunits I and II Genes(PRPS1 and PRPS2)to Different Regions of the X Chromosome and Assignment of Two PRPS1-Related Genes to Autosomes 7 and 9." Somat.Cell Mol.Genet.15. 29-37 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kudoh,J.;Shimizu,N.;et al.: Jpn.J.Human Genet.33. 315-324 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Arai,N.;Kudoh,J.;Shimizu,N.;et al.: Journal of Immunology. 142. 274-282 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Li,K.;Kudoh,J.;Shimizu,N.;et al.: EMBO Journal.

    • Related Report
      1988 Annual Research Report
  • [Publications] Minoshima,S.;Kudoh,J.;Shimizu,N.;et al.: Cytogenetics and Cell Genetics.

    • Related Report
      1988 Annual Research Report
  • [Publications] Ohno,S.;Kudoh,J.;Shimizu,N.;et al.: Cytogenetics and Cell Genetics.

    • Related Report
      1988 Annual Research Report
  • [Publications] Taira,M.;Kudoh,J.;Shimizu,N.;et al.: Somatic Cell and Molecular Genetics.

    • Related Report
      1988 Annual Research Report
  • [Publications] J. Kudoh, S. Minoshima, K. Hashinaka, C. Nishio, M. Yamada, Y. Shimizu and N. Shimizu: Ninth International Workshop of Human Gene Mapping 9 Abstracts. 497 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] J. Kudoh, S. Minoshima, A. Ullrich, Y. Shimizu and N. Shimizu: Ninth International Workshop of Human Gene Mapping 9 Abstracts. 498 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Taira, J. Kudoh, S. Minoshima, M. Tatibana, Y. Shimizu and N. Shimizu: Ninth International Workshop of Human Gene Mapping 9 Abstracts. SUPPL.5 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] N. Shimizu, S. Minoshima, J. Kudoh, K. Kawasaki, R. Fukuyama and M. Maekawa: Cold Spring Harbor Symposium Abstracts.

    • Related Report
      1987 Annual Research Report
  • [Publications] M. Taira, T. Iizasa, S. Ishijima, K. Yamada, M. Tatibana, J. Kudoh, S. Minoshima and N. Shimizu: 14th International Congress of Biochemistry Abstracts.

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Minoshima, J. Kudoh, R. Fukuyama, M. Maekawa, Y. Takebe, T. Yokota,N. Arai, K. Arai and N. Shimizu: XVIth International Congress of Genetics Abstracts.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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