| Project/Area Number |
62470112
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
反応工学
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Takeshi Nagoya University, Faculty of Engineering, Professor, 工学部, 教授 (10043324)
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| Co-Investigator(Kenkyū-buntansha) |
IIJIMA Shinji Nagoya University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (00168056)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Recombinant DNA / Cross-flow filtration / Two step culture / Bioreactor / Tryptophan promoter / 乳酸菌 / グルコン酸菌 / 瀘過型培養 |
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| Research Abstract |
For the effective production of useful gene products, two step culture of recombinant microorganisms is very effective. We used the dioreacter equipped with cross-flow filtration system and developed this culture method. With the microorganisms bering trp- and pho- promoter expression vectors, vectors, we succeeded in the inducible expression and overproduction of gene products using this bioreactor.
The gene expression from trp promoter could be well induced in the absence of glucose. We developed an novel on-line glucose monitoring system and controlled the glucose concentration, which enabled the effective induction of gene expression. In addition to this, we developed a novel on-line system to estimate the product concentration and could improve the effectiveness of lactic acid and gluconic acid fermentation.
We successflully cultured obligately aerobic bacteria with this filtration bioreactor and showed that the bioreactor could be used for aerobic microorganism.
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