| Project/Area Number |
62470119
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Nagoya University |
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Principal Investigator |
AKAZAWA Takashi Nagoya University, Faculity of Agriculture, 農学部, 教授 (20023400)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Hirokazu Nagoya University, Isotope center, アイソトープセンター, 助手 (80170348)
TAKABE Tetsuko Nagoya University, Faculity of Agriculture, 農学部, 講師 (60089852)
山口 淳二 名古屋大学, 農学部, 助手 (10183120)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | -amylase / rice / oligosaccharide chair / biosynthesis / intracellular / transport / イネ液体培養系 / ジベレリン / 糖タンパク質 / 複合型糖鎖 / 高マンノース型糖鎖 / 分泌型糖タンパク質 / 細胞内輸送系 |
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| Research Abstract |
Since rice seed -amylase is shown to be a typical Asn-N-linked secretory glycoprotein, the excised rice seedlings have been proved to be a feasible material to study the mechanism of the biosynthesis as well as the intracellular transport of the secretory protein in plant cells. It has been shown that -amylase molecules bearing both high mannose-type and complex-type oligosaccharide chains are secreted from the rice scutellar tissues, and a hypothetical mechanism concerning the stryctural modification of the oligosaccharide moiety linked the -amylase polypeptide chain in the golgi compartment has been proposed.
In order to further study the detailed enzymic machineries for rice -amylase biosynthesis, we have been currently employing the liquid suspension-cultured cells. Using this suspension-cultured cells, we got several data as follows.
1. Oligosaccharide structure in secreted -amylase is (GlcNAc)_2Man_3GlcNAc_2 or GlcNAcMan_3GlcNAc_2. These structures are plant specific complex type oligosaccharide.
2. Complex type oligosaccharide chain in secreted -amylase is produced from the precursor containing high-mannose type oligosaccharide chain.
3. When the oligosaccharide modification is inhibited by some environmental factors, secreted -amylase has high-mannose type oligosaccharide chain.
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