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Molecular Analysis of Structural Changes of Plant Nuclear DNA.

Research Project

Project/Area Number 62480007
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHokkaido University

Principal Investigator

TANIFUJI Shigeyuki  Hokkaido Univ., Fac. of Sci., Professor, 理学部, 教授 (50000774)

Co-Investigator(Kenkyū-buntansha) KATO Atsushi  Hokkaido Univ., Fac. of Sci., Lecturer, 理学部, 講師 (90177428)
加藤 敦之  北海道大学, 理学部, 助手 (89077428)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsSatellite DNA / 5.8S rRNA gene / Intergenic spacer of rDNA / Imitation-Methionine tRNA gene / Mutated histidine tRNA gene / Arabidopsis thaliana / Vicia faba / Pisum sativum / 5.8SrRNA遺伝子 / rDNA内部転写スペ-サ- / ヒスチジンtRNA遺伝子 / ニンジン / ソラマメγRNA遺伝子 / エンドウマメγRNA遺伝子 / γRNA転写開始点 / 植物γDNAスペーサーの変異性 / シロイヌナズナアラニンtRNA遺伝子 / シロイヌナズナメチオニンtRNA遺伝子 / フェニールアラニンtRNA遺伝子 / ニンジンrRNA遺伝子 / ソラマメrRNA遺伝子 / 植物rRNAの転写開始点 / 植物のrDNA大スペーサーの変異性 / 植物tRNAの二次元ゲル電気泳動 / エンドウマメtRNAの僞遺伝子
Research Abstract

For an understanding of structural differentiation of plant nuclear genomes, we analyzed molecular structure of highly repeated DNA sequences, rRNA genes and tRNA genes in carrot (Daucus carota), broad bean (Vicia faba), pea (Pisum sativum) and Arabidopsis thaliana.
Our previous studies-have shown that when carrot root disks were cultured in the presence of auxin, certain portions of cells initiated DNA replication synchronously, and a GC-rich repeated DNA component began to replicate earlier than the main band DNA. By the use of benzoylated naphthoylated DEAE-cellulose column chromatography, we obtained evidence that this early replicating DNA remained at the state of the double-stranded molecules containing substantial single-stranded regions for much longer period than the main DNA component.
We determined the nucleotide sequence of the 18S rRNA coding region of broad bean rRNA gene.
We made a phylogenetic tree of 5.8S rRNA sequences of some representative eukaryotes including broad bean and carrot plants with which the sequences of their genes were determined by us.
The complete nucleotide sequence of the intergenic spacer (IGS) of rRNA gene was determined for broad bean and pea plants. This spacer region showed a considerable sequence similarity between these two legume plants. However, the patterns of molecular organization were different from each other. The pea IGS contained only one type of repeated sequence, whereas the broad bean IGS contained five kinds of repeated sequences. The sequence of 17 bp surrounding the imitation site of transcription which was tentatively determined by Sl mapping experiment was identical between broad bean and pea plants, and also similar, though not identical, to the corresponding regions of maize and wheat rRNA genes.
The nucleotide sequences of a gene for methionine-initiation tRNA and a mutated gene for histidine tRNA of Arabidopsis thaliana were also determined.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takahiro TAIRA: "Difference between two major Size classes of carrot rDNA repeating units is due to reiteration of about 460 bp sequences in the large spacer." Molecular and General Genetics. 213. 170-174 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takahiro TAIRA: "Difference in timing of replication and maturation of nascent DNA fragments between satellite and main band DNA during the S phase of the first cell cycle in cultured carrot root explants." Japanese journal of Genetics. 64. 79-89 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yukihiko YOKOTA: "Nucleotide sequences of the 5.85rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA" Journal of Molecular Evolution. 29. 294-301 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuhito AKAMA: "Nucleotide sequence of a methionine initiator tRNA gene of Arabidopsisthaliana." Plant Molecular Biology. 13. 599-600 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuhito AKAMA: "Nucleotide sequence of a putative pseudogene of histidine tRNA from Arabidopsis thaliana" Plant Molecular Biology. 13. 721-722 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Atsushi KATO: "The structure of the nontranscribed spacer and external transcribed region of the Vicia faba and Pisum sativum rRNA genes." 投稿中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takahiro Taira: "Differences between two major size classes of carrot rDNA repeating units is due to reiteration of about 460 bp sequences in the large spacer." Mol. Gen. Genet.213:170-174, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takahiro Taira: "Differences in timing of replication and maturation of nascent DNA fragments between satellite and main band DNA during the Sphase of the first cell cycle in cultured carrot root explants." Jpn. J. Genet. 64:79-89, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yukihiko Yokota: "Nucleotide sequences of the 5.8S rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA." Jour. Mol. Evol. 29:294-301, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuhito Akama: "Nucleotide sequence of a methionine initiator tRNA gene fo Arabidopsis thaliana." Plant Mol. Biol.13:599-600, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuhito Akama: "Nucleotide sequence of a putative pseudogene of histidine tRNA from Arabidopsis thaliana." Plant Mol. Biol.13:721-722, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Atsushi Kato: "The structure of the nontranscribed spacer and external transcribed region of the Vicia faba and Pisum sativum rRNA genes."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Takahiro TAIRA: "Differences in timing of replication and maturation of nascent DNA fragments between satellite and main band DNA during the S phase of the first cell cycle in cultured carrot root explants." Japanese Journal of Genetics. 64. 79-89 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yukihiko YOKOTA: "Nucleotide sequences of the 5.8S rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA。" journal of Molecular Evolution. 29. 294-301 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuhito AKAMA: "Nucleotide sequence of a methionine initiator tRNA gene of Arabido-psis thaliana。" Plant Molecular Biology. 13. 599-600 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kazuhito AKAMA: "Nucleotide sequence of a putative pseudogene of histidine tRNA from Arabidopsis thaliana." Plant Molucular Biology. 13. 721-722 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Atsushi KATO: "The structure of the nontranscribed spacer and external transcribed region of the Vicia faba and Pisum sativum rRNA genes."

    • Related Report
      1989 Annual Research Report
  • [Publications] Atsushi KATO: "The nucleotide sequence of the 18S rRNA gene in Vicia faba。"

    • Related Report
      1989 Annual Research Report
  • [Publications] Kimitake,YAKURA: Jpn.J.Genet.62. 325-332 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takahiro,TAIRA: Mol.gene.Genet.213. 170-174 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukihiko,YOKOTA: J.Mol.Evol.in press. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takahiro,TAIRA: Jpn.J.Genet.in press. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 谷藤茂行: "赤沢・杉浦・西村編「植物の細胞生物学的研究法」(植物の重複性遺伝子)" 共立出版, 277-287 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kimitaka YAKURA: Japanese Journal of Genetics. 62. 325-332 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Takahiro TAIRA: Molecular General Genetics. (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 谷藤茂行: 蛋白質核酸酵素(別冊)・植物の細胞生物学的研究法. 30. 277-287 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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