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The role of the cell wall in nuclear positioning in plant cells

Research Project

Project/Area Number 62480009
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionOsaka University

Principal Investigator

SHIBAOKA Hiroh  Dept. of Biol., Fac. of Sci., Osaka Univ. Professor, 理学部, 教授 (60087054)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsNuclear positioning / Cytoskeleton / Actin filaments / Microtubules / Cell wall / タバコ培養細胞 / 細胞固定法 / 核の移置決定機構
Research Abstract

1. In spherical interphase tobacco By-2 cells cultured in the presence of NAA and benzyl-adenine, the nucleus is present in the central region of the cell being tethered by the cytoplasmic strands in which actin filaments are present, but microtubules are not. The disruption of actin filaments in the cytoplasmic strands by cytochlasin alters the position of the nucleus, but that of microtubules by propyzamide does not alter the position of the nucleus. From these findings, we reach the conclusion that actin filaments, but not microtubules, are involved in determining the position of the nucleus in interphase tobacco BY-2 cells.
2. In protoplasts isolated from tobacco BY-2 cells, the nucleus is present in the central region of the protoplast. But, as the protoplasts regenerate cell walls, the nucleus changes its position fom the central region to the cell periphery. This nuclear migration is inhibited by 2,6-dichlorobenzonitrile, an inhibitor of cellulose biosynthesis. As pro-toplasts treated with 2,6-dichlorobenzonitrile do not regenerate cell walls, we reach the conclusion that the presence of the cell wall is necessary for the nucleus to migrate from the central region to the cell periphery.
3. In premitotic tobacco BY-2 cells cultured in the presence of 2,4-D, the nucleus is present in the central region of the cell being tethered by the cytoplasmic strands which contain both action filaments and microtubules. Neither the disruption of actin filaments by cytochlasin nor that of microtubules by propyzamide alters the position of the nucleus. The nucleus changes its position only when both actin filaments and microtubules are dis-rupted. From these results we reach the conclusion that both actin filaments and micro-tubules are involved in the nuclear positioning in premitotic tobacco BY-2 cells.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] KAKIMOTO,T.: Plant and Cell Physiology. 28. 1581-1585 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KATSUTA,J.: Plant and Cell Physiology. 29. 403-413 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] AKASHI,T.: Plant and Cell Physiology. 29. 1053-1062 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KAKIMOTO,T.: Protoplasma[Supplementum]. 2. 95-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sonobe,S.: Protoplasma.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 柴岡弘郎: 蛋白質核酸酵素.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KATSUTA, J.: "The role of the cytoskeleton and the cell wall in nuclear positioning in tobacco BY-2 cells" Plant and Cell Physiology. 29. 403-413 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] AKASHI, T.: "Effects of propyzamide on tobacco cell microtubules in vivo and in vitro" Plant and Cell Physiology. 26. 1053-1062 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KAKIMOTO, T.: "Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells" Protoplasma [Supplementum]. 2. 95-103 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] SONOBE, S.: "Cortical fine actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment with m-maleido-benzoyl N-hydrosuccinimide ester" Protoplasma. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] SHIBAOKA, H.: "The cytoskeleton in cytokinesis in plant cells (in Japanese)" Protein, Nucleic Acid and Enzyme. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KATSUTA,J.: Plant and Cell Physiology. 29. 403-413 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] AKASHI,T.: Plant and Cell Physiology. 29. 1053-1062 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] KAKIMOTO,T.: Protoplasma[Supplementum]. 2. 95-103 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sonobe,S.: Protoplasma.

    • Related Report
      1988 Annual Research Report
  • [Publications] 柴岡弘郎: 蛋白質核酸酵素.

    • Related Report
      1988 Annual Research Report
  • [Publications] KAKIMOTO,T.: Plant and Cell Physiology. 28. 1581-1585 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] KATSUTA,J.: Plant and Cell Physiology. 29. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] KAKIMOTO,T.: Protoplasma.

    • Related Report
      1987 Annual Research Report
  • [Publications] AKASHI,T.: Plant and Cell Physiology.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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