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Study on Molecular mechanisms regulating S phase in sea urchin embryo at cleavage stage.

Research Project

Project/Area Number 62480015
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionHIROSHIMA UNIVERSITY (1988)
The University of Tokyo (1987)

Principal Investigator

SHIMADA Hiraku  Professor, Zoological Institute, Faculty of Science, Hiroshima University, 理学部, 教授 (70011559)

Co-Investigator(Kenkyū-buntansha) AKASAKA Koji  Associate professor, Zoological Institute, Faculty of Science, Hiroshima Univers, 理学部, 助教授 (60150968)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsSea urchin / AP4A / DNA replication / Nuclear matrix / Arylsulfatase / Gene cloning / S phase / 発生 / 細胞周期 / ウニ / 卵割期 / ジアデノシン四リン酸 / トポイソメラーゼ
Research Abstract

In most oviporous animals, the S phases during cleavage stage are extremely short, and little, if any, gap phases are detectable in the cell cycles at this stage of development. This project has been planned to elucidate the mechanism specifically regulating the S phases in the cells of cleavage embryos of sea urchin.
(A) Role of deadenosine tetraphosphate (AP4A) on the regulation of s phase initiation. We have previously reported that the level of the soluble AP4A in sea urchin embryos at cleavage stage fluctuates cyclically during the cell cycle, drastically decreasing at the beginnning of S phase and increasing again during the S phase. We found that the decrease in the AP4A level is not due to enzymatic hydrolysis of this substance. A part of the free AP4A becomes bound to the nuclear matrix proteins at the beginning of S phase. The AP4A-binding activity of nuclear matrix of sea urchin embryos is most prominent at the beginning of S phase and become lowest during the other period of … More cell cycle. Gel electrophoretic analysis of the AP4A-binding proteins from nuclear matrix revealed the presence of two distince proteins, 23 kda protein and 70 kda protein. It seems that the 70 kda protein corresponds to nuclear lamins. We are now trying to purify the AP4A-binding protein of 23 kda.
(B) Regulation of DNA replication at cleavage stage. It is generally believed that the extremely short S phases in cleavage-stage embryos of sea urchin are supported byactivation of many extra replication origins on genomic DNA. In the cells of abult sea urchin which replicate their DNA relatively slowly, these extra origins would be in an inactive state. To confirm this hypothesis, it is needed to compare the numbers of active origins on the DNA fragmenst with known length and nucleotide sequnce which are obtained from various stages of development. For this purpose, we have isolated the arylsulfatase gene (approx. 20 kbp) from sea urchin embryos by the conventional gene-cloning procedure starting from purification of this enzyme. Less

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hiroshi,Sasaki: Comparative Biochemistry and Physiology. 88B. 147-152 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizue,Morioka: Experimental Cell Research. 169. 57-62 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiraku,Shimada: Development,Growth and Differentiation. 29. 417-425 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi,Sasaki: European Journal of Biochemistry. 177. 9-13 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Sasaki, K. Akasaka, H. Shimada and T. Shiroya: "Purification and characterization of arylsulfatase from sea urchin (Hemicentrotus pulcherrimus) embryos." Comparative Biochemistry and Physiology. 88B. 147-152 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizue, Morioka; H. Shimada: "AP4A-hydrolyzing activity in sea urchin embryos." Experimental Cell Research. 169. 57-62 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiraku, Shimada: "DNA replication and its regulation in cleavage embryos of sea urchin." Development, Growth and Differentiation. 29. 417-425 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi, Sasaki; K. Yamada; K. Akasaka; H. Kawasaki; K. Suzuki; A. Saito; M. Sato; H. Shimada: "cDNA cloning, nucleotide sequence and expression of the gene for arylsulfatase in the sea urchin (Hemicentrotus pulcherrimus) embryo." European Journal of Biochemistry. 177. 9-13 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hiroshi Sasaki: Comparative Biochemistry and Phyiology. 88B. 147-152 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Mizue Morioka: Experimental Cell Research. 169. 57-62 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hiraku Shimada: Development,Growth and Differentiation. 29. 417-425 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hiroshi,Sasaki: European Journal of Biochemistry. 177. 9-13 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 森岡瑞枝: Experimental Cell Research. 169. 57-62 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 嶋田拓: Development,Growth and Differentiation. 29. 417-425 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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