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Mechanism of determination of the flagellar beat plane.

Research Project

Project/Area Number 62480016
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionUniversity of Tokyo

Principal Investigator

TAKAHASHI Keiichi  Zoological Institute, Faculty of Science, University of Tokyo, 理学部, 教授 (40011481)

Co-Investigator(Kenkyū-buntansha) SHINGYOJI Chikako  Zoological Institute, Faculty of Science, University of Tokyo, 理学部, 助手 (80125997)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsFlagella / Sperm / Beat plane / Microtubule / Central micotubule / Dynein / 細胞運動
Research Abstract

Eukaryotic flagellar movements are based on sliding between doublet microtubules in the axoneme. To induce a planar bend, the amount of sliding within the axoneme should be controlled so that the axoneme behaves as if it were composed of two groups of doublets. To study the mechanism of the formation of the planar bends, we applied a new experimental procedure to sea urchin sperm flagella. We have found that the beat plane of the flagella can be rotated over several cycles under an experimental condition in which the micropipette which held the sperm head with suction is vibrated and the plane of this imposed vibration is rotated. Spontaneous recovery of the beat plane occurs after the cessation of the vibration. As many as 11 cycles of revolution of the beat plane were followed by such spontaneous reverse rotations in live flagella.
The beat plane of conventional Triton models did not rotate for more than one revolution, but that of reactivated sperm that had been demembranated with a solution containing CHAPS and Nonidet P-40 rotated along with the plane of vibration for more than 10 revolutions. The response of the flagellum after a cessation of the vibration varied with the direction of rotation of the vibration plane: counterclockwise rotations were followed by a complete unwinding but clockwise rotations were followed by a partial unwinding. The initial velocity of unwinding was the same in both directions and was a function of the number of revolutions of the imposed winding. since the polystyrene beads attached to the axoneme did not rotate around the axoneme during the rotation, it is unlikely that the 9 doublets rotate around the axonemal axis. These results indicate that the flagellum can change the beat plane by changing the pattern of active sliding within the axoneme, possibly in association with rotation of the central tubules.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] 高橋景一、真行寺千佳子: 生物物理. 27. 145-150 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高橋景一、村上彰: 動物生理. 4. 38-57 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 村上彰、高橋景一: 宇宙生物科学. 2. 228-237 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa,K.;K.Takahashi: Cell Struct.Funct.13. 193-205 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa,K;K.Takahashi: Yamada Conference XI:Energy Transduction in ATPases Osaka. 210-211 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 真行寺千佳子、高橋景一: 生体の科学. 39. 92-97 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 真行寺千佳子;高橋景一: 生体の科学. 39. 491-492 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ishii,N.;F.Mitsumori;K.Takahashi;A.W.M.Simpson,;C.C.Ashley: Muscle Energetics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamada,A.;N.Ishii;T.Shimmen;K.Takahashi: J.Muscle Res.Cell Motility.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takahashi, K.; A. Murakami: "Animal physiology at colleges: a questionnaire-based survey." Dobutsu Seiri. 4. 38-57 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takahashi, K.; C. Shingyoji: "'Rotation' in eukaryotic flagellar movement." Biophysics. 27. 145-150 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murakami, A.; K. Takahashi: "Gravitaxis of Paramecium." Biological Sciences in Space. 2. 228-237 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa, K.; K. Takahashi: "Force-velocity relation of microtubule sliding in demembranated sperm flagella of the sea urchin." Cell Struct. Funct.13. 193-205 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa, K.; K. Takahashi: "The mechanical power developed by dynein arms in sliding microtubules of flagella." Yamada Conference XI: Energy Transduction in ATPases, Yamada Science Foundation, Osaka.11. 210-211 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takahashi, K.; A. Murakami: "Animal physiology at colleges: a questionnaire-based survey." Dobutsu Seiri. 4. 38-57 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Takahashi, K.; C. Shingyoji: "'Rotation' in eukaryotic flagellar movement." Biophysics. 27. 145-150 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Murakami, A.; K. Takahashi: "Gravitaxis of Paramecium." Biological Sciences in Space. 2. 228-237 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa, K.; K. Takahashi: "Force-velocity relation of microtubule sliding in demembranated sperm flagella of the sea urchin." Cell Struct. Funct.13. 193-205 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Oiwa, K.; K. Takahashi: "The mechanical power developed by dynein arms in sliding microtubules of flagella." Yamada Conference XI: Energy Transduction in ATPases, Yamada Science Foundation, Osaka.11. 210-211 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 村上彰,高橋景一: 宇宙生物科学. 2. 228-237 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Oiwa,K;K.Takahashi: Cell Struct.Funct.13. 193-205 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Oiwa,K;K.Takahashi: Yamada Conrerence XI:Energy Transduction in ATP ases (eds.Y.Mukohata,M.F.Morales and S.Fleischer) Yamada Science Foundation,Osaka. 210-211 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 真行寺千佳子,高橋景一: 生体の科学. 39. 92-97 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 真行寺千佳子,高橋景一: 生体の科学. 39. 491-492 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Ishi,N.;F.Mitumori;K.Takahashi;A.W.M.Simpson;C.C.Ashley: Muscle Energetics (ed.R.Paul) ,Alan R.Liss,NY.

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamada,A.;N.Ishii;T.Shimmen;K.Takahashi: J.Muscle Res.Cell Motility.

    • Related Report
      1988 Annual Research Report
  • [Publications] 高橋景一: 生物物理. 27. 11-16 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 真行寺千佳子: 生体の科学. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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