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Molecular Mechanism of Transdifferentiation in the Pigmented Epithelial Cell

Research Project

Project/Area Number 62480020
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionOkazaki National Research Institutes

Principal Investigator

EGUCHI Goro  Okazaki National Research Institutes, National Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (80022581)

Co-Investigator(Kenkyū-buntansha) AGATA Kiyokazu  Okazaki National Research Institutes, National Institute for Basic Biology, Rese, 基礎生物学研究所, 助手 (70167831)
KODAMA Ryuji  Okazaki National Research Institutes, National Institute for Basic Biology, Rese, 基礎生物学研究所, 助手 (90161950)
WATANABE Kenji  Okazaki National Research Institutes, National Institute for Basic Biology, Asso, 基礎生物学研究所, 助教授 (00079691)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsReparative regeneration / Transdifferentiation / Dedifferentiation / Redifferentiation / Gene expression and regulation / Morphogenetic factor / 遺伝子の発現とその調節 / 修退再生 / 形態形成の調節因子
Research Abstract

Based on studies of Wolffian lens regeneration in the newt, in which the lens can be regenerated from the iris pigmented epithelium, we have investigated molecular mechanisms regulating the processes of transdifferentiation of pigmented epithelial cells (PECs) to lens cells. The following findings have been established for three years from 1987 to 1989.
(1) It has been proved by application of cell culture procedures developed in our laboratory that PECs dissociated from fully-grown human eyes readily transdifferentiate into lens phenotype in the manner observed in chick embryo PECs. In addition, we succeeded in isolation of permanent cell lines from dedifferentiated human PECs, and also in establishing the basis of a unique in vitro cell culture system which must be highly useful for studying cellular and molecular mechanisms of transdifferentiation in human cells.
(2) We have completely described the pattern of expression of both pigment cell- and lens cell-specific genes in the whole sequence of lens transdifferentiation in chick embryo PECs. It has also shown that expression of these cell type-specific genes are transcriptionally regulated in the processes of reddiferentiation of multipotent dedifferentiated PECs.
(3) Two different genes were isolated from chick embryo PECs. It has been assumed from the result of structural analyses that both genes code for a protein responsible for regulation of dedifferentiation and transdifferentiation of PECs.
(4) We have found a cell surface glycoprotein which stabilize the differentiated state of PECs of the newt in situ. Although this glycoprotein is not specific to the PECs but widely detected in mesoderm-originated tissues and some of ectoderm-originated epithelia, this cell surface molecule plays an essential role in dedifferentiation of iris PECs in the process of lens regeneration in the newt.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Mochii,M.,Takeuchi,T.,Kodama,R.,Agata,K.,and Eguchi,G: "The expression of melanosomal matrix protein in the transdifferentiation of pigmented epithelial cells into lens cells." Cell Differentiation. 23. 133-142 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Mochii,M.Agata,K.,Kobayashi,H.,Yamamoto,T.S.and Eguchi,G: "Expression of gene coding for a melanosomal matrix prptein transcriptionally regulated in the transdifferentiation of chick embryo pigmented epithelial cells." Cell Differentiation. 24. 67-74 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Eguchi,G.: "Cellular and molecular background of Wolffian lens regeneration." Cell Differentiation. 25(Supl). 147-158 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Watanabe,K.,Aoyama,H.,Tamamaki,H.,Sonomura,T.,Okada,T.S.,Eguchi,G.and Nojo,Y.: "An embryonic pineal body as a multipotent system in cell differentiation." Development. 103. 17-26 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Morota,H.,Takeuchi,T.,Suzuki,S.,Maeda,K.,Yamada,K.,Eguchi,G.and Kimata,K.: "Aortic endothelial cells synthesize a large shondroitin sulphate proteoglycan capable of binding to hyaluronate." Biochemical Journal. 265. 61-68 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kodama,R.,Agata,K.,Mochii,M.and Eguchi,G.: "Partial amino acid sequence of the major intrinsic protein(MIP)of the chicken lens deduced from the nucleotide sequence of a cDNA clone." Experimental Eye Research(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kitamura,Y.: "Ontogenesis of α_2ーadenoceptor coupling with GTPーbinding proteins in the rat telencephalon." Journal of Neurochemistry. 53. 249-253 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Orita,H.: "Aortic endothelial cells synthesize a large chondroitin sulphate proteoglycan capable of binding to hyaluronate." Biochemical Journal. 256. 61-68 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kodama,R.: "Partial amino acid sequence of the major intrinsic protein(MIP)of the chicken lens deduced from the nucleotide spquence." Experimental Eye Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Goro Eguchi.: Cell Differentiation. 25(Supl.). 147-158 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Makoto Mochii.: Cell Differentiation. 23. 133-142 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Makoto Mochii.: Cell Differentiation. 24. 67-74 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kebji Watanabe.: Development. 103. 17-26 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Makoto Mochii: Cell Defferentiation. 23. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Makoto Mochii: Cell Differentiation. 24. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kenji Watanabe: Development. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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