| Project/Area Number |
62480042
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Hokkaido University |
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Principal Investigator |
UYEDA Ichiro Faculty of Agriculture,Hokkaido University, 農学部, 助教授 (10113523)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Plant reovirus / Rice dwarf virus / Rice black-streaked dwarf virus / Rice ragged stunt virus / Genome structure / イネラギッドスタントウイルス / cDNA / 末端構造 |
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| Research Abstract |
Genome structures of rice dwarf viurs (RDV), rice black streaked dwarf virus(RBSDV), and rice ragged stunt virus (RRSV) were analyzed. Complete nucleotide sequences of RDV S9 and S10. RRSV S9, and RBSDV S10 were determined. Segment specific inverted repeats located at both 5' and 3' terminal regions were found in these segments indicating that this structure plays an importnat role in replication of the viruses. Terminal sequences of RDV were analyzed. It was found that the last four nucleotides (-UGAU3') at the 3' terminus were common to all the segments except for the S 9, where the fourth from the terminus was changed to C from U. 5' terminal sequence of RDV S3-S12 was determined to be 5'GGUAAA-- or 5'GGCAAA--. These conserved sequences were also common or similar to that of wound tumor virus , another member of plant reovirus subgroup 1. This indicates common ancestral origin of the two viruses. The conserved terminal sequence also indicates that it is indispensable in replicationof the viral genome. Furthermore presumed terminal conserved sequences of RRSV and RBSDV deduced from the sequence analyses of RRSV segment 9 and RBSDV segment10 indicated that they are different from each other.
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