• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on Host Recognition Mechanisms in Symbiotic Nitrogen-fixing System

Research Project

Project/Area Number 62480054
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionThe University of Tokyo

Principal Investigator

MARUYAMA Yoshiharu  Faculty of Agriculture, The University of Tokyo, 農学部, 教授 (60011863)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1988: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1987: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsSymbiotic nitrogen fixation / Host specificity / Lectin / Leghemoglobin / Non-nodulating soybean / Astragalus sinicus L. / 熱帯性マメ科植物 / プラスミド / レ ンゲ / 根粒菌非着生種 / 根粒菌 / 根粒菌着生種
Research Abstract

(1) Host specificity of Rhizobium sp. Astragalus sinicus L. the rhizibium showed high host specificity against Astragalus sinicus L. by cross inoculation test. Lectin was isolated from the seed of Astragalus and tested the reaction with various other root nodule bacteria. The lectin showed the reactivity only with Rhizobium sp. Astragalus. The lectin was purified about 30 times of specific activity by ammonium sulfate fractionation, DEAE- and CM- cellulose column chromatography. Mega-plasmids were isolated from R. sp. Kohnosu and R. sp. Nagano of Astragalus and named pRko 1, pRko 2, pRna 1 and pRna 2. Their molecular weight was 72.7 201,112 and 293 Md. pRko 1 was hybridized with nif D.
(2) Non-nodulating strain of soybean (rj 1). The difference of lectin of root between ri 1 and wild strain (Rj 1) was examined by using antilectin antibody. No difference was observed. PAGE pattern of proteins extracted from roots was not also any significant differences between rj 1 and Rj 1.
(3) Symbiotic system in some tropical cultivated legumes. Root nodule bacteria were isolated from Vigna sinensis, Psophocarpus tetragonolobus and Vigna umbelleta and their chracters, host specificity, nitrogenfixing activity were examind. Leghemoglobins were also investigated.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 石井忠雄: 日本土壌肥料学雑誌. 58. 7-11 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] W.Y.Shieh: Can.J.Microbiol.34. 886-890 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S.Sekiguchi: J.Basic Microbiol.28. 529-539 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F.Tagawa: Plant and Cell Physiol.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T.M.Merestela: Agric.Biol.Chem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. Ishii: "Estimation of nodulation by inoculum strain with marker strain method." Jap. J. Soil Science and Plant Nutr.58. 7-11 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] W. Y. Shieh: "New marine nitrogen-fixing bacteria isolated from an eelgrass (Zostera marina) bed." Can. J. Microbiol.34. 886-890 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] S. Sekiguchi: "Assimilatory reduction of nitrate in Rhizobium meliloti." J. Basic Microbiol.28. 529-539 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] F. Tagawa: "Characterization of non-nodulating soybean." Plant and Cell Physiol.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] T. M. Merestela: "Symbiotic nitrogen fixation by some tropical cultivated legumes." Agric. Biol. Chem.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] W.Y.Shieh: J.Gen.microbiol.134. 1821-1825 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] W.Y.Shieh: Can.J.Microbiol.34. 886-890 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S.Sekiguchi: J.Basic Microbiol.28. 529-530 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] F.Tagawa: Plant and Cell Physiol. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] T.M.Merestela: Agric.Biol.Chem.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] U.Yoon: Agric Biol.Chem.(1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yoshiharu MARUYAMA(W.Y.SHIEH;U.SIMIDU): J.Gen.Appl.Microbiol.33(4). 321-330 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 丸山芳治: 日本土壌肥料学雑誌. 58(1). 7-11 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 田川不二夫;丸山芳治: 日本農業化学会誌(1988年度大会講演要旨集). 62(3). (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 尹雄漢;丸山芳治: 日本農芸化学会誌(1988年度大会講演要旨集). 62(3). (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi