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Analysis of expression mechanisms for trans-split gene in a liverwort, Marchantia polymorpha chloroplasts.

Research Project

Project/Area Number 62480056
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionKyoto University

Principal Investigator

OHYAMA Kanji  Associate Professor, Faculty of Agriculture, Kyoto University, 農学部, 助教授 (40135546)

Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordschloroplast genome / liverwort / trans-splicing / ribosomal protein / exon / intron
Research Abstract

A number of chloroplast genes are interrupted by introns and thus require post-transcriptional RNA splicing for the gene expression. Chloroplast introns belong to either group I or group II depending on their secondary structures, which was first elucidated in mitochondoria. This report deals with trans-splicing of chlorolast ribosomal protein S12 gene. The chloroplast gene RPS12 for ribosomal protein S12 in a liverwort, M. polymorpha, is aplit into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the N-terminal portion of the S12 protein is far from exons 2 and 3 for the C-terminal portion on the opposite DNA stand. S1-nuclease protection analysis and northern hybridization with RNA isolated from the licerwort chloroplasts showed that: (i) the exons 1 and 2-3 of the rps12 gene with the neighboring genes were transcribed separately, (ii) the trans-splicing of intron 1 occurred after the processing of two primary transcripts to two per- mRNAs, (iii) there was no particular order for the splicing of intron 1 (trans) and intron 2 (cis) in the rps12 gene. We propose a bimolecular interaction model for trans-splicing by assuming that intermolecular base pairings between two pre-mRNA result in the formation of the structure typical of group II introns except for disruption in the loop III region. This structure could be constructed in intron 1 of tobacco rps12 gene.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Fukuzawa,H.: FEBS Letters. 220. 61-66 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama,K.: Trends in Biochemical Sciences. 13. 19-22 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama,K.: Photosynthesis Research,. 16. 7-22 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohchi,T.: The EMBO J.,. 7. 885-891 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama,K.: J.Mol.Bol.,. 203. 281-298 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Fukuzawa, H.: "Splicing of group II introns 1 in mRNA coding for cytochrome b6 and subunit IV in the liverwrt M. polymorpha chloroplast genome" FEBS Letters. 220. 61-66 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama, K.: "Nerwly identified groups of genes in chloroplasts" Trends in Biochemical Sciences. 13. 19-22 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama, K.: "Gene organization and newly identified groups of genes of the chloroplast genome from a liverwort, Marchantia polymorpha." Photosynthesis Research,. 16. 7-22 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kohchi, T.: "Divergent mRNA transcription the chlorolast psbB operon." The EMBO J.,. 7. 885-891 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ohyama, K.: "Structure and organization of Marchantia polymorpha chloroplast genome I. Cloning and gene identification." J. Mol. Bol.,. 203. 281-298 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] H.Fukuzawa;T.Yoshida;T.Kohchi;T.Okamura;K.Ohyama: FEBS Lettets. 220. 61-66 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Ohyama;T.Kohchi;T.Sano;Y.Yamada: Trends in Biochemical Sciences. 13. 19-22 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hideya Fukuzawa: FEBS Letters. 220. 61-66 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kanji Ohyama: Trends in Biochemical Sciences. 13. 19-22 (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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