• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Classification and molecular genetics of Chlamydia psittaci

Research Project

Project/Area Number 62480086
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionGifu University

Principal Investigator

HIRAI Katsuya  Gifu University, Agriculture, Professor, 農学部, 教授 (30021702)

Co-Investigator(Kenkyū-buntansha) FUKUSHI Hideto  Gifu University, Agriculture, Assistant Professor, 農学部, 講師 (10156763)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1989: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsChlamydia psittaci / Monoclonal antibody typing of C. psittaci / Genetic diversity of C. psittaci / Outer membrane protein of C. psittaci / Genetic relationship of C. psittaci strains / DNA-DNA hybridization in C. psittaci
Research Abstract

1 ) A total of 77 Chlamydia psittaci strains of avian, human, and mammalian origin were grouped into four serovars with 11 monoclonal antibodies recognizing the lipopolysaccharide and the major outer membrane protein antigens. The avian and human strains, which were closely related to each other, were distinct from the mamalian strains. Immunological typing of C. psittaci with monoclonal antibodies seems practical.
2 ) Immunochemical properties of the major outer membrane protein (MOMP) of 16 strains of Chlamydia psittaci isolated from psittacine birds, budgerigars, a pigeon, turkeys, humans, cats, a muskrat, sheep, and cattle and a strain of C. trachomatis, L2/434/Bu, were compared by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and by immunoblotting analysis with hyperinnunized rabbit antisera to strains of parrot, turkey, feline, and bovine origin. The MOEPs of the strains showed variation in molecular weights and immunological specificities. Fifteen of the C. psittaci … More strains were classified into two avian and two mammalian types based on immunological specificity of the MOMP, whereas the other strain was not classified in this study. Immunological classification based on specificity of the MOMP by immunoblotting proved to be a valuable method to classify various strains of C. psittaci.
3 ) Genetic relationships were reported for Chlamydia psittaci derived from psittacine birds, pigeons, turkeys, humans, cats, muskrats, cattle, and sheep and for C. trachomatis, including representative strains of the three biovars, through physical analysis of genomic DNA including DNA fingerprinting with restriction endonuclease SalI, DNA-DNA hybridization in solution with Sl nuclease, and Southern analysis with genomic DNA probes. A total of 26 strains were divided into four groups of C. psittaci and two groups of C. trachomatis, on the basis of DNA fingerprints. The six groups of Chlamydia spp. were related to host origin: two avian groups (Avl and Av2), one feline and muskrat group (Fel), one ruminant group (Rul), one C. trachomatis biovars trachoma and lymphogranuloma group (CtHu), and one C. trachomatis mouse biovar group (CtMo), although an ovine abortion strain belonged to the avian group Av2. DNA-DNA hybridization assay and Southern analysis with genomic DNA probes indicated three DNA homology groups in the genus Chlamydia: an avian-feline group (groups Avl, Av2, and Fel), a ruminant group (group Rul), and a C. trachomatis group (groups CtHu and CtMo). Furthermore, the Southern analysis indicated that the homologous sequences (DNA homology of at least 14%) within the avian-feline group were distributed along the whole genome, whereas the homologous sequences (DNA homology of less than 24%) among the three DNA homology groups were localized in distinct regions of the genome DNA. These results suggest that Chlamydia spp. are derived from a common ancestor and have diverged into various groups showing restricted host ranges as a natural characteristic and that the species C. psittaci should be differentiated into groups related to host origin and DNA homology. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Fukushi,H.,Nojiri,K.and Hirai,K.: "Monoclonal antibody typing of Chlamydia psittaci strains derived from avian and mammalian species" Journal of Clinical Microbiology. 25(10). 1978-1981 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi,H.and Hirai,K.: "Immunochemical diversity of the major outer membrane protein of avian and mammalian Chlamydia psittaci" Journal of Clinical Microbiology. 26(4). 675-680 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi,H.and Hirai,K.: "Genetic diversity of avianand mammalian Chlamydia psittaci related to their host origin" Jounalof Bacteriology. 171(5). 2850-2855 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kubo,H.and Hirai,K.: "Purification and characterization of the major outer membrane protein of Chlamydia psittaci" Infection and Immunity(in press). (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hirai,K.and Fukushi,H.: "Immunological subtyping of Chlamydia psittaci derived from birds and animals with monoclonal antibodies to the major outer membrane protein" American Journal of Veterinary Research(in press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平井克哉,福士秀人: "クラミジア感染症-特に分類と疫学(分担課題)獣医学1989 伊沢久夫,清水悠紀臣 編集代表" 近代出版, 28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 平井克哉: "リッケッチア類およびクラミジア類(分担課題)新編獣医微生物学 梁川良 編集代表" 養賢堂, 35 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi, H., Nojiri, K. and Hirai, K.: "Monoclonal antibody typing of Chlamydia psittaci strains derived from avian and mammalian species." Journal of Clinical Microbiology, 25(10): 1978-1981, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi, H. and Hirai, K.: "Immunochemical diversity of the major outer membrane protein of avian and mammalian Chlamydia psittaci." Journal of Clinical Microbiology, 26(4): 675-680, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi, H. and Hirai, K.: "Genetic diversity of avian and mammalian Chlamydia psittaci strains and relation to host origin." Journal of Bacteriology, 171(5): 2850-2855, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fukushi,H.,Nojiri,K.and Hirai,K.: "Monoclonal antibody typing of Chlamydia psittaci strains derived from avian and mammalian species" Journal of Clinical Microbiology. 25(10). 1978-1981 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukushi,H.and Hirai,K.: "Immunochemical diversity of the major outer membrane protein of avian and mammalian Chlamydia psittaci" Journal of Clinical Microbiology. 26(4). 675-680 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukushi,H.and Hirai,K.: "Genetic diversity of avian and mammalian Chlamydia psittaci related to their host origin" Journal of Bacteriology. 171(5). 2850-2855 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kubo,H.and Hirai,K.: "Purification and characterization of the major outer membrane protein of Chlamydia psittaci" Infection and Immunity. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hirai,k.and Fukushi,H.: "Immunological subtyping of Chlamydia psittaci derived from birds and animals with monoclonal antibodies to the major outer membrane protein" American Journal of Veterinary Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平井克哉・福士秀人: "クラミジア感染症-特に分類と疫学(分担課題)獣医学1989伊沢久夫・清水悠紀臣編集代表" 近代出版, 28 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平井克哉: "リッケッチア類およびクラミジア類(分担課題)新編獣医微生物学梁川良編集代表" 養賢堂, 35 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fukushi,H.;Hirai,K.: Journal of Clinical Microbiology. 26(4). 675-680 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukushi,H.;Hirai,K.: Journal of Bacteriology. 172. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kubo,H.;Hirai,K.: Infection and Immunity. 58. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukushi,H.;Hirai,K.: Journal of Clinical Microbiology. 28. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平井克哉,福士秀人: "クラミジア感染症ー特に分類と疫学 獣医学1989 伊沢久夫・清水悠紀臣編集代表" 近代出版, 28-56 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 平井克哉: "ワケツチア類およびクラミジア類 新編獣医微生物学 梁川良編集代表" 養賢堂, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fukushi,H.: Journal of Clinical Microbiology. 25. 1978-1981 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fukushi,H.: Journal of Clinical Microbiology. 26. in press (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fukushi,H.: Journal of Clinical Microbiology. 26. in press (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 熊本悦明: "クラミジア感染症の基礎と臨床,分担課題:鳥類および哺乳動物におけるクラミジア感染症" 金原出版株式会社, 25 (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi