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Studies on Transmission of Hemorrhagic Fever with Renal Syndrome (HFRS) Virus in Rodents

Research Project

Project/Area Number 62480087
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

HASHIMOTO Nobuo  Faculty of Veterinary Medicine, Hokkaido University, 獣医学部, 教授 (60082103)

Co-Investigator(Kenkyū-buntansha) MORITA Chiharu  Department of Zoonoses, National Institute of Health, 獣疫部, 室長 (50072369)
TAKASHIMA Ikuo  Faculty of Veterinary Medicine, Hokkaido University, 獣医学部, 助教授 (30002083)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsHemorrhagic fever with renal syndrome (HFRS) / HFRS virus / Hantavirus / Rodent / Transmission of HFRS virus / Epizootiology of HFRS / Ecology of HFRS virus / Viral Zoonoses / げっ歯類媒介性人畜共通伝染病 / ドブネズミ / ウィルス性ズノーシス / げつ菌類媒介性人畜共通伝染病 / HFRSウイルス / げつ歯類 / HFRSウイルスの伝播機序 / HFRSウイルス病原性 / ウイルス性人畜共通伝染病 / げつ歯類由来人畜共通伝染病
Research Abstract

The mode of HFRS virus transmission among rodents was examined by comparing the results of epidemiological surveys of urban rats in endemic areas and experimental infection of laboratory rats. The results are as follows.
1) The urban rats in the endemic focus have shown the age-dependent acquisition of infection. HFRS virus was mostly isolated from lung tissue in adult rats showing high antibody titers.
2) A total four sero-positive small field mice, two Clethrionomys rufocanus bedfordiae, one Apodemus argenteus hokkaidi and one Apodemus speciosus ainu, were detected around the endemic focus indicating an evidence of transmission of HFRS virus from the infected urban rats to indigenous field mice.
3) Two strains of HFRS virus from Rattus, SR-11 and KI-262, showed identical antigenicity but deferred in virulence against newborn rats. SR-11 strain isolated from a laboratory rat developed clinical signs and died at about 18 days after inoculation. KI-262 strain from an urban rat in an endemi … More c focus, did not show any clinical signs. Necrosis of neurons in the brain tissue occurred in the rats infected with SR-11, while it was mild in the animals infected with KI-262.
4) When rats-were inoculated with SR-11, sero-conversion rate was almost similar in newborn and adult rats regardless of inoculation routes.
5) IgG antibody was transferred from immune dams to their offspring prenatally as well as postnatally. IgG antibody was detected in the fetus sera. In newborn rats, maternal IgG antibody reached a peak titer at two weeks after birth, then declined and disappeared at about 8 weeks of age. The protective effect of maternal antibody remained in 10 week-old rats.
6) Antibody dependent enhancement (ADE) of the infection of HFRS virus was studied using macrophage-like cell lines. ADE of the infection occurred with monoclonal antibodies (MAbs) to both Gl and G2 envelope glycoproteins, but not with MAbs to nucleocapsid protein. Antigenic determinants related to hemagglutination and virus neutralization were found to cause ADE of the infection Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Maeda,T.,Arikawa,J.,Takasima,I.and Hashimoto,N.: "Enzyme linked immunosorbent assay for detection of antibody against hemorrhagic fever with renal syndrome virus in sera from experimentally infected rats" Jpn.J.Vet.Res.35. 215-219 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang,X-K.,Takashima,I.and Hashimoto,N.: "Characteristics of passive immunity against hatavirus infection in rats" Arch.Virol.105. 235-246 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang,X-K.,Takashima,I.and Hashimoto,N.: "Role of maternal antibody in protection from hemorrhagic fever with renal syndrome virus infection in rats" Arch.Virol.103. 235-265 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang,X-K.,Takashima,I.and Hashimoto,N.: "Comparison of virulence between two strains of Rattus serotype hemorrhagic fever with renal(HFRS) syndrome virus in newborn rats" Microbiol.Immunol. 33. 195-205 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Morita,Ch.,Takashima,I.Matuura,Y.and Kitamura,T.: "The prevalence of hantaan viruses among Rattus norvegicus in Tokyo in the 1960's" J.vet.Med.36. 557-558 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akikawa,J.,Schmaljohn,A.L.,Dalrymple,J.M.and Schmaljohn,S.S: "Characterization of hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies" J.gen.Virol.70. 615-624 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawamura,K.,Zhang,X-K.,Akikawa,J.,Takashima,I.,Dempo,K.and Hashimoto: "Susceptibilities of laboratory and wild rodents to the Rattus-or Apodemus-derived Hantavirus" Arch.Virol(Submit). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto,N.,Yao,J-S.and Akikawa,J: "Antibody dependent enhancement of the hantavirus infection to macrophage-like cell lines" VIIIth International Congress of Virology,Berlin. 8. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto,N.: "Epidemiological surveys of hantavirus infections among urban rats in an endemic focus in Japan" 23rd Working Conference on Viral Diseases,Japan-U.S. Cooperative Medical Science Program,Annapolis. 23. 18 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akikawa,J.and Hashimoto,N.: "Characterizations of hantaan virus envelope glycoprotein antigenic determinants related to virus neutralization and pathogenesis in suckling mice" 1st International Conference of HFRS,Seoul. 1. 3 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto,N.: "Hemorrhagic fever with renal syndrome(Kerean hemorrhagic fever)" Seminal on Laboratory Animal Science,ROC-Japan,Taipei. 1. 3-4 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Akikawa,N.,Hashimoto,N.and Takashima,I.: "Epidemiological studies of hemorrhagic fever with renal syndrome" VIIth International Congress of Virology,Alberta,Canada. 7. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Maeda, T., Arikawa, J., Takashima, I.and Hashimoto, N.: "Enzyme linked immunosorbent assay for detection of antibody against hemorrhagic fever with renal syndrome virus in sera from experimentally infected rats" Jpn.J.Vet.Res.35 215-219 (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang, X.-K., Takashima, I.and Hashimoto, N.: "Role of maternal antibody in protection from hemorrhagic fever with renal syndrome virus infection in rats" Arch. Virol., 103, 253-265 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang, X.-K., Takashima, I.and Hashimoto, N.: "Characteristics of passive immunity against hatavirus infection in rats" Arch. Virol., 105, 235-246(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang, X.-K., Takashima, I.and Hashimoto, N.: "Comparison of virulence between two strains of Rattus serotype hemorrhagic fever with renal syndrome (HFRS) virus in newborn rats" Microbiol. Immunol., 33, 195-205(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Morita, Ch., Takeuchi, I., Matsuura, Y.and Kitamura, T.: "The prevalence of hantaan viruses among Rattus norvegicus in Tokyo in the 1960's" J. Vet. Med. 36, 557-558(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Arikawa, J., Schmaljohn, A.L., Dalrymple, J.M.and Schmaljohn, S.S.: "Characterization of hantaan virus envelope glycoprotein antigenic deteminants defined by monoclonal antibodies" J.gen.Virol., 77, 615-624(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawamura, K., Zhang, X.-K., Arikawa, J., Takashima, I., Dempo, K.and Hashimoto, N.: "Susceptibilities of laboratory animals and wild rodents to the Rattus-or Apodemus-derived hantavirus" Arch. Viro.(1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto, N., Yao, J-S.and Arikawa, J.: "Antibody dependent enhancement of the hantavirus infection to macrophage-like cell lines" VIIIth International Congress of Virology, Berlin, 8(1990).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto, N.: "Epidemiological surveys of hantavirus infections among urban rats in an endemic focus in Japan" 23rd Working Conference on Viral Diseases, Japan-U.S.Cooperative Medical Science Program, 23, 18(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Arikawa, J.and Hashimoto, N.: "Characterizations of hantaan virus envelope glycoprotein antigenic determinants related to virus neutralization and pathogenesis in suckling mice" 1st International Conference of HFRS, Seoul, 1, 3(1989).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hashimoto, N.: "Hemorrhagic fever with renal syndrome (Korean hemorrhagic fever)" Seminar on Laboratory Animal Science, ROC-Japan, Taipei, 1,3-4, (1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Arikawa, J., Hashimoto, N.and Takashima, I.: "Epidemiological studies of hemorrhagic fever with renal syndrome" VIIth International Congress of Virology, Alberta, Canada, 7(1987).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Zhang,X.-K.,Takashima,I.and Hashimoto,N.: "Cheracteristics of passive immunity against hantavirus infection in rats" Arch,Virol.105. 235-246 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Zhang,X.-K.,Takashima,I.and Hashimoto,N.: "Comparison of virulence between two strains of Rattus serotype hemorrhagic fever with renel syndrome(HFRS)virus in newborne rats" Microbiol.Immunol.33. 195-205 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Morita,Ch.,Takeuchi,Y.Matsuura,Y.and Kitamura,T.: "The prevalence of hantaan viruses among Rattus norvegicus in Tokyo in the 1960's" J.Vet.Med.36. 557-558 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Arikawa,J.,Schmaljohn,A.L..Dalrymple,J.M.and Schmaljohn,S.S.: "Characterization of hantaan virus enevelope glycoproteinantigenic determinants defined by monoclonal antibodies" J.gen.Virol. 70. 615-624 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawamura,K.,Zhang,X.-K.,Arikawam,J.Takashima.,I.Dempo,K.and Hashimoto,N.: "Susceptibilities of laboratory and wild rodents to the the Rattus-or Apodemus-derived hantavirus" Arch.virol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hashimoto,N.,Yao,J-S,and Arikawa,J.: "Antibody dependent enhancement of the hantavirus infection to macrophage-like cell lines" VIIIth International Congress of Virology,Berlin. 8. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Zhang,X-K,et al.: Arch.Virol. 103. 253-265 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Zhang,X-K,et al.: Microbiol.Immunol.33. 195-205 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Zhang,X-K,et al.: Arch.Virol.106. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Imamura,I.,et al: 22nd Joint Working Conference on Viral Diseases,Japan-US Cooperative Med.Scie.Program. 22. 35-36 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kawamura,K.,et al: Acta Virol.Submitt. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 藍明陽 他: 第106回 日本獣医学会総合要旨集. 106. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Maeda,T;Arikawa,J.;Takashima,I.;Hashimoto,N.: Jpn.J.Vet.Res.35. 215-219 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 張献奎;高島郁夫;橋本信夫: 日本ウイルス学会総会抄録. 35. 236 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 張献奎;高島郁夫;橋本信夫: 第104回日本獣医学会総会要旨集. 104. 199 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 橋本信夫: 臨床獣医. 5. 21-23 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hashimoto,N;Zhang,X.K.: Working Conference on Viral Diseases,U.S.-Japan Cooperative Medical Science Program,Monterey. 21. 25 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hashimoto,N.: ROC-Japan Seminar on Laboratory Animal Science,Taipei. Abst. 21-22 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 有川二郎: "獣医学:腎症候性出血熱" 近代出版, 200-204 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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