| Project/Area Number |
62480096
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyushu University |
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Principal Investigator |
YAMAMOTO Torao Kyushu University,Faculty of Medicine,Professor, 医学部, 教授 (80037324)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Keiichirou Kyushu University,Faculty of Medicine,Lecturer, 医学部, 講師 (20172398)
WASANO Kojirou Kyushu University,Faculty of Medicine,Assistant Professor, 医学部, 助教授 (90117292)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Endothelial cells / Quick-freeze replica / Deep-etching replica / Plasmalemmal Vesicles / Actin filaments / 蛍光顕微鏡 / 電子顕微鏡 / 凍結割断レプリカ / デスミン / ビメンチン / アクチン / エッチングレプリカ / 縞状構造 / クラスリン |
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| Research Abstract |
The present study was carried out to clarify the structural basis, particularly molecular arckitectural basis, of the functions of endothelial cells by using ultrathin section, freeze-fracture replica and fluorescence microscope techniques.
The cytoplasmic surface of plasmalemmal vesicles in aortic endothelial cells was examined in quick-freeze, deep-etching replicas. The cytoplasmic surface membranes of small vesicles (60-80 nm in diameter) which are usually observed as smooth vesicles in section were associated with striped patterns. These stripes were composed of several ridges or strands of 6-10 nm in width, and some were crossed. These striped structures were enhanced by treatment with myosin subfragment-1 or-phalloidin, thus indicating a possible relation to actin filaments. These striped structures may be suggested to be involved in the formation and transport of smooth vesicles. The freeze-fracture replicas of the luminal surface membrane of the endothelial cells did not show any regular distribution patterns. The actin filaments consisting the cytoskelet were different in nature from those of medial smooth muscle cells as demonstrated by immuno-fluorescence microscopy.
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