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Migration of macrophages in lymphoid tissues in relation to the immune response

Research Project

Project/Area Number 62480097
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

KOTANI Masahiko  Kumamoto University, medical school, Professor, 医学部, 教授 (20025521)

Co-Investigator(Kenkyū-buntansha) MATSUNO Kenjiro  Kumamoto University, medical school, Lecturer, 医学部, 講師 (20094047)
EZAKI Taichi  Kumamoto University, medical school, Assistant, 医学部, 助手 (10128259)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMacrophages / Immune responses / Spleen / Thymus / Autoimmune / 胸腺 / マクロファージ / 抗体産生細胞
Research Abstract

1) Migration of carbon-laden macrophages in lymphoid tissues.
(1) Spleen ---Splenic carbon-laden macrophages injected into the splenic artery of recipient rats migrated through the white pulp from the marginal sinus into the lymph follicles. In contrast to ED1(+),2(+),3(-) resident scavenger macrophages in the red pulp, the phenotype of the majority of carbon-laden macrophages migrating into the white pulp were ED1(+), 2(-), 3(-). Most of them were also specific in morphology. These results suggest the presence of a distinct subpopulation of macrophages which actively migrate into the splenic white pulp including the germinal centers.
(2) Thymus ---A great increase in the permeability of carbon and carbon-laden macrophages from blood vessels into the thymic parenchyma was observed both in the cortex and medulla, particularly at the corticomedullary junction in the thymus of autoimmune mice. These results suggest the possible role of the great increased blood vessel permeability in the thymus on the aetiology and pathogenesis of autoimmune disease.
2) Role of antigen-laden macrophages on the development of antibody-forming cells.
By new established methods of double or triple immunostainings, close association of the specific antibody-forming cells (AFC) with the antigen-laden marginal metallophils (MM) or forming cell clusters with ED2-positive macrophages in the outer periarterial lymphoid sheath (PALS) was demonstrated. These results led to the proposal of a hypothesis that the antigen-laden MM together with ED2-positive macrophages constitute an immunoproliferative microenvironment for the plasmacellular reaction by accumulating the antigen-specific B-cell lineage and promoting these cells to differentiate into the AFC and to proliferate in the outer PALS.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 松野健二郎: "脾臓のマクロファ-ジ" 細胞. 20. 23-28 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 小谷正彦: "Increased vascular permeability in the thymus of the autoimmune New Zealand mouse" Journal of Anatomy. 161. 83-93 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 大森淳: "Migration of carbon-laden peritoneal macrophages into the thymus of autoimmune New Zealand mice" Journal of Anatomy. 165. 9-17 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 松野健二郎: "Splenic outer periarterial lymphoid sheath (PALS) :An immunoproliferative microenvironment constituted by antigen-laden marginal metallophils and ED2-positive macrophages in the rat" Cell and Tissue Research. 257. 459-470 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮川和久: "Localization in the rat spleen of carbon-laden macrophages introduced into the splenic artery:A subpopulation of macrophages entering the white pulp" Anatomical Record(印刷中). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 松野健二郎: "Histophysiology of Immune System" Plenum Publishing Corporation, 5 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kenjiro Matsuno: "Macrophages in the spleen (Jap.)." Cell, 20, 23-28, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kenjiro Matsuno: "Plenum Publishing Corporation, Histophysiology of the Immune System." 1988, 5 pages.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Masahiko Kotani: "Increased vascular permeability in the thymus of the autoimmune New Zealand mouse." Journal of Anatomy, 161, 83-93, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Jun Ohmori: "migration of carbon-laden peritoneal macrophages into the thymus of autoimmune New Zealand mice." Journal of Anatomy, 165, 9-17, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kenjiro Matsuno: "Splenic outer periarterial microenvironment constituted by antigen-laden marginal metallophils and ED2-positive macrophages in the rat." Cell and Tissue Research, 257, 459-470, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuhisa Miyakawa: "Localization in the rat Spleen of carbon-laden macrophages introduced into the splenic artery : A subpopulation of macrophages entering the white pulp." Anatomical Record, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 大森淳: "Migration of carbon-laden peritoneal macrophages into the thymus of autoimmune New Zealand mice" Journal of Anatomy. 165. 9-17 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 松野健二郎: "Splenic outer periarterial lymphoid sheath(PALS):An immunoproliferative microenvironment constituted by antigen-laden marginal metallophils and ED2-positive macrophages in the rat" Cell and Tissue Research. 257. 459-470 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮川和久: "Localization in the rat spleen of carbon-laden macrophages introduced into the splenic artery:A subpopulation of macrophages entering the white pulp" Anatomical Record. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 松野健二郎: 細胞. 20. 23-28 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 小谷正彦: Jouranl of Anatomy. 161. 83-93 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 松野健二郎: 細胞. 20. 23-28 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 小谷正彦: Journal of Anatomy. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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