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Three dimensional analysis of dendrites of the CNS neurons.

Research Project

Project/Area Number 62480099
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionSchool of Human Sciences, Waseda University (1988-1989)
Okazaki National Research Institutes (1987)

Principal Investigator

HAMA Kiyoshi  School of Human Sciences, Waseda University, Professor., 人間科学部, 教授 (90028267)

Co-Investigator(Kenkyū-buntansha) ARII Tatsuo  National Institute of Physiological Science, Assistant Professor., 生理学研究所, 助教授 (10109267)
小坂 俊夫  岡崎国立共同研究機構, 生理学研究所, 助教授 (00126054)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsDendritic spine / CNS neuron / 3-D analysis / HVEM / Golgi preparation / 超高圧電子顕微鏡 / 樹状突起棘 / 樹状突起スパイン / ラット歯状回顆粒細胞 / 樹状突起刺の三次元定量解析 / 超高圧電子顕微鏡による立体画像 / コンピューター画像解析
Research Abstract

We have developed a method of extracting three dimensional information quantitatively from high voltage electron microscope stereo images. To improve the accuracy of the result, we introduced into Hitachi HVEM H-1250 a rotation free zoom optical system and a side entry rotation tilt stage with a feed back control mechanism for the tilt angle. The microscope is also equipped with an image intensifier in combination with a real time image processor to reduce the beam damage to the specimen.
Number, length and diameter of dendritic spines of the granule cell in the rat dentate gyrus were measured with the aid of this system. 5 m thick Golgi preparations were used for the measurement.
Spine densities of 3.4, 2.3, and 2.0 per 1mu m at proximal (P), middle (m), and distal (d) portion of the dendrite respectively were obtained, which were 1.6-2.0 fold of the previous light microscopical report. Since the diameter of the dendrite decreases from proximal to distal portion, the spine densities per unit surface area of the dendrite was about equal, 0.8/mu m^2, throughout the dendritic tree.
Spines of various shapes , calyx shape,mushroom shape, club shape , thin thread-like etc. were found , however the large stubby spines which were reported to be 20-40 % by light microscopy were rare, less than 2%. Mean three dimensional spine length were 1.25mu m, 1.26mu m, and 1.24 mu m at p, m, and d portions respectively, which were about 1.4 times longer than those measured in two dimensions.
The surface area of the spines were measured using morphological parameters , and it was found that the total surface areas of the dendrite was doubled by the addition of the spines at each dendritic portion.
It became evident that the resolution of the light microscope was not sufficient for the precise morphometry of the dendritic spine.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] K.Hama and T.Arii: "Three dimensional amalysis of high voltage electron microscope tilt imges:methods and problems" Journal of Electron Micnoscopy Technique. 6. 185-192 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Arii and K.Hama: "Method of extracting three-diminsional information from HVTEM stereoimages of biological materials" Journal of Electron Microscopy. 36. 177-195 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama,T.Anii,and T.Kosaka: "Three-dimensional morphometrical study of dendritic spines of the granule cell in the ratdentate gyrus with HVEM stereoimages" Journal of Electron Microscopy Technique. 12. 80-87 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama: "Biological application of high voltage electron microscopy" Journal of Electron Microscopy. 38. 156-162 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 有井達夫,浜清: "超高圧電顕による立体観察" 細胞. 21. 188-193 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 有井達夫,浜清: "超高圧電顕を用いた立体写真の三次元画像解析" 細胞. 21. 262-268 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama(分担): "High voltage electron microscopy in cell biology.in X-Ray Microscopy in Biology and Medicine.Edited by K.Shinohara et al." Japan Scientific societies Press/Springer-Verlag, 339 277-283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama and T.Arii: "Three-dimensional analysis of high voltage electron microscope tilt images: Methods and problems." J.Electr.Microsc.Tech., 6, 185-192, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Arii and K.Hama: "Method of extracting three-dimensional information from HVTEM stereo images of biological materials." J.Electr.Microsc., 36, 177-195, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama, T.Arii and T.Kosaka: "Three-dimensional morphometrical study of dendritic spines of the granule cell in the rat dentate gyrus with HVEM stereo images." J.Electr.Microsc.Tech., 12, 80-87, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama: "Biological application of high voltage electron microscopy." J.Electr.Microsc., 38, s156-s162, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama: "High voltage electron microscopy in cell biology." in X-Ray Microscopy in Biology and Medicine, K.Shinohara et al. eds, Japan Sci. Soci. Press - Springer Verlag, pp 277-283, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Arii and K.Hama: "Stereoscopic observation by high voltage electron microscopy. in Japanese." Cell, 21, 188-193, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.Arii and K.Hama: "Three dimensional analysis of HVTEM stereo images. in Japanese." Cell, 21, 262-268, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Hama and T.Arii: "Threeーdimensional morphometical study of dendritic spines of the granule cell in the rat dentate gyrus with HVEM stereo images" Journal of Electron Miroscopy Technique. 12. 80-87 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hama: "Biological application of high voltage electron microscopy" Journal of Electron Microscopy. 38. S156-S162 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 有井達夫,浜清: "超高圧電顕による立体観察" 細胞. 21. 188-193 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 有井庁夫,浜清: "超高圧電顕を用いた立体写真の三次元画像解析" 細胞. 21. 262-268 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hama: "Threeーdimensional organization of glia cell proces as revealed by high voltage electron microscope stereoーscopy:perineuronal astrocyte in the brain stem reticular formation of rats." Anatomy and Embrylolgy.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hama: "High voltage electron microscopy in cell biology,in XーRay Microscopy in Biology and Medicine,K.Shinohara et,al,eds." Japan Scientific Society PressーSpringer Verlag, 277-283 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hama.;T.Kosaka.;T.Arii.: J.Electron Microscope Technique.

    • Related Report
      1988 Annual Research Report
  • [Publications] 有井達夫、浜清: Cell.

    • Related Report
      1988 Annual Research Report
  • [Publications] 有井達夫、浜清: Cell.

    • Related Report
      1988 Annual Research Report
  • [Publications] K.Hama.: Proceeding of 3rd International Conference on Synchrotoron Radiation Instrumentation:X-ray microscopy.

    • Related Report
      1988 Annual Research Report
  • [Publications] Kiyoshi Hama;Tatsuo Arii: Journal of Electrom Microscopy Technique. 6. 185-192 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tatsuo Arii;Kiyoshi Hama: Journal of Electrom Microscopy. 36. 177-195 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kiyoshi Hama;Tatsuo Arii;Tshio Kosaka: Journal of Electrom Microscopy Technique.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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