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Menbrane phospholipase c activation and signal transduction in relation to change of receptor-linkage mechanism

Research Project

Project/Area Number 62480123
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKeio University

Principal Investigator

KATO Ryuichi  School of Medicine, Keio University, 医学部, 教授 (40112685)

Co-Investigator(Kenkyū-buntansha) SASAKAWA Nobuyuki  School of Medicine, Keio University, 医学部, 助手 (20187107)
NAKAKI Toshio  School of Medicine, Keio University, 医学部, 助手 (30164148)
YAMAMOTO Satoshi  School of Medicine, Keio University, 医学部, 助教授 (50138129)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordschromaffin cells / Ca^<++> mobilization / inositol phosphate / nicotinic receptor / 牛副腎髄質培養クロマフィン細胞 / イノシトール三リン酸 / 細胞内遊離Ca^<2+> / サイクリックGMP / 培養日数
Research Abstract

The mechanism of functional shift from muscarinic to nicotinic cholinergic receptors involved in cytosolic Ca^<++> mobilization and inositol triphosphate (IP_3) during the primary culture of adrenal chromaffin cells was studied. At 0.5 day in culture, muscarine, a specific agonist for muscarinic receptors, caused a greater effect on intracellular Ca^<2+> mobilization and the accumulation of Ins(1,3,4)P_3 than did the nicotinic-specific agonist nicotine. On the contrary, at 5 days, nicotine produced a greater effect on the accumulation of Ins(1,3,4)P_3 and intracellular calcium mobilization than did muscarine. Furthermore, at 0.5 day, the muscarinic antagonist atropine strongly inhibited the increase in InsP_3 accumulation that was induced by the nonspecific agonist carbachol, whereas at 5 days the inhibitory effect of atropine was greatly lowered. On the other hand, the nicotinic receptor antagonists hexamethonium and - tubocurarine showed a much higher inhibitory potency at 5 days compared with 0.5 day in culture. Binding experiments with a muscarinic ligand excluded the possibility that the reduction in muscarinic effects on InsP_3 and intracellular Ca^<2+> mobilization were due to disappearance of the muscarinic receptor itself. These data show that cholinergic receptors linked to the accumulation of InsP_3 and Ca^<2+> mobilization functionally shift from muscarinic to nicotinic during primary culture of adrenal chromaffin cells.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Sasakawa,N.: FEBS lett.223. 413-416 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakaki,T.: Biochem.J.251. 397-403 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa,N.: Biochem.Pharmacol.37. 2485-2487 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa,N.: Cell.Signal.1. 75-84 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa,N.: J.Neurochem.52. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山本慧: "ホルモンと臨床" 医学の世界社, 29-35 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 加藤隆一: "刺激-分泌連関におけるシグナルトランスダクションとCa^<2+>動態" 日本薬理学会, (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa, N.: "Inositol trisphosphate accumulation by high K^+ stimulation in cultured adrenal chromaffin cells." FEBS lett.223. 413-416 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakaki, T.: "Functional shift from muscarinic to nicotinic sholinergic receptors involved in inositol trisphosphate and cyclic GMP accumulation during the primary culture of adrenal chromaffin cells." Biochem. J.251. 397-403 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa, N.: "Effects of islet-activation protein on the catecholamine release, Ca^<2+> mobilization and inositol trisphosphate formation in cultured adrenal chromaffin cells" Biochem. Pharmacol.37. 2485-2487 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa, N.: "Calcium uptake-dependent and -independent mechanisms of inositol trisphosphate formation in adrenal chromaffin cells: comparative studies with high K^+, carmamylcholine and angiotensin II." Cell. Signal.1. 75-84 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa, N.: "Stimulation by ATP of inositol trisphosphate accumulation and calcium mobilization in cultured adrenal chromaffin cells." J. Neurochem.52. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, S.: "Catecholamine secretion and calcium" in "Hormone and clinics" (Vol. 36). 29-35 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sasakawa,N.: Biochem. Pharmacol.37. 2485-2487 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasakawa,N.: Cell. Signal.1. 75-84 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasakawa,N.: J. Neurochem.52. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 山本慧: "ホルモンと臨床" 医学の世界社, 29-35 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 加藤隆一: "刺激-分泌連関におけるシグナルトランスダクションとCa^<2+>動態" 日本薬理学会, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sasakawa.N;Makaki.T;Yamamoto.S;and.Kato.R: FEBS Letters. 223-2. 412-416 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakaki.T;Sasakawa.N;Yamamoto.S;and.Kato.R: Biochem.J. > (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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