| Project/Area Number |
62480137
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HATAKEYAMA Shigeru Tokyo Medical and Dental University, Medical Faculty Proffessor, 医学部, 教授 (30045926)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Takahiko Tokyo Medical and Dental University, Medical Faculty Ass. Proffessor, 医学部, 助手 (90197799)
EISHI Yoshinobu Tokyo Medical and Dental University, Medical Faculty Ass. Proffessor, 医学部, 助手 (70151959)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Spermatogenic disorder / Autoimmunity / Anti-Sertoli cell autoantibody / 精細管基成膜 / 精細管基底膜 |
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| Research Abstract |
Human autoantibody to Sertoli cells was detected in normal human sera. This IgM-type autoantibody was undetectable during neonatal period and was found in 11.5% of 365 serum samples taken from adult healthy persons of both sexes. This human autoantibody to Sertoli cells exhibited quite the same target-organ specificity and:multi-organ reactivity as those of the murine monoclonal autoantibody(IgM class) to Sertoli cells (TM-1: WHO registered code T43) which has already been demonstrated capable of inducing experimental spermatogenic disturbance in mice. These human and murine autoantibodies could recognize testicular self-antigens of molecular weight 67,000 and 23,000 in Sertoli cells.
Monoclonal antibody to basement membrane of the seminiferous tubules (TM-2: WHO registered code T44) allowed the autoantibody to reach the Sertoli cells across the blood-testis barrierr. Autoantibody with the same target-tissue specificity as TM-2 was also demonstrated in human sera, both recognizing type-IV collagen molecules as target self-antigens of the testis.
Thus, human spermatogenic disturbance could be easily induced even in healthy individuals, once a functional impairment of Sertoli cells and blood-testis barrierr composed mainly of basement membrane of seminiferous tubules were induced by either these naturally-occuring autoantibodies or other toxic damages of blood-testis barrierr.
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