| Project/Area Number |
62480138
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Okayama University Medical School (1988)
Kochi Medical School (1987) |
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Principal Investigator |
TAKAHASHI Kiyoshi Associate Professor, Okayama University Medical School, 医学部, 助教授 (90101815)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | S100 protein / T-lymphocyte / Malignant lymphoma / CTL / Dendritic cell / S100蛍白 / 細胞障害性T細胞 / dendritic cell / T細胞白血病 |
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| Research Abstract |
I found that human S100beta^+ T-lymphocytes (s100TL) belong to suppressor/cytotoxic T-lymphocyte subset and can be stimulated by concanavalin A (Virchows Arch B, 53: 375, 1987). I also found that S100TL tend to markedly decresase in number in peripheral blood of patients with advanced cancer, suggesting their important role in tumor immunity (Blood, 70:214, 1987). I found a case with leukemia of S100TL and proposed "S100beta positive T-cell leukemia/lymphoma" as a new category of human T-cell malignancy through assessing similar cases reported previously (Blood, 71:1299, 1988). I found that S100^+ T-leukemia cells exhibited natural killer (NK)-like non-specific cytotoxity and expressed gamma/delta T-cell antigen receptor (TcR) heterodimer (submitted). I made a normal S100TL clone by stimulation of normal human peripheral blood lymphocytes with alloantigen and interleukin-2.The S100TL clone named Yt-CL6 exhibited allospecific cytotoxity and expressed alpha/beta TcR heterodimer. These findings indicate that S100TL belong to cytotoxic T-lymphocyte (CTL) subset. I also found that the S100^+ T-leukemia cells and Yt-CL6 cells (a normal S100TL clone) exhibited dendritic morphological changes upon stimulation with phorbol ester (TPA). The results suggest that S100TL may be a special type of CTL which can convert their morphology in dendritic shapes resembling Thy-1^+ dendritic epidermal cells in murine epidermis.
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