| Project/Area Number |
62480147
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Chiba University |
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Principal Investigator |
KOJIMA Somei Chiba University School of Medicine, 医学部, 教授 (00009622)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Hidekazu Chiba University School of Medicine, 医学部, 助手 (00110304)
KOBAYASHI Masashi Chiba University School of Medicine, 医学部, 助手 (80009654)
NIIMURA Munetoshi Chiba University School of Medicine, 医学部, 助教授 (60059095)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Schistosoma japonicum / monoclonal IgE / protective immunity / antibody-dependent cytotoxicity / eosinophils / macrophages / 防御抗原 / 教酸球 |
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| Research Abstract |
Experiments were carried out to study on the role of a mouse monoclonal IgE antibody in protective immunity to Schistosoma japonicum infection. The monoclonal IgE antibody (SJ18 .1) has been shown to elicit PCA in the rat and to recognize a 97 kDa molecule expressed on the surface of schistosomula. 1. Purification of SJ18 .1: The antibody was purified as a single peak by using the FPLC system with anion exchange chromatography and gel filtration. Affinity chromatography will be carried out for isolation of the 97 kDa molecule. 2. Induction of protective immunity and its cellular mechanisms: (1) Protection to a challenge infection was observed in mice treated with intraperitoneal injections of SJ18 .1 in the migratory phase of schistosomula from the skin to lung. Lung schistosomula were found to be resistant to the monoclonal IgE antibody-dependent cytotoxicity. (2) It was demonstrated in vitro that the IgE-dependent damage to S.japonicum schistosomula was mediated by both of eosino phils and macrophages. (3) Immunization with x-irradiated cercariae induced a significant resistance to a challenge infection with S.japonicum in DBA/2 mice but not in C57BL/6. The low responsiveness of C57BL/6 was contrary to observations previously reported on this strain with S.mansoni infection. The low ability of macrophages in the monoclonal IgE-dependent damage to schistosomula suggested the low responsiveness of C57BL/6 was at least partly due to some defects in macrophage functions. 3. Analysis and gene cloning of the protective antigen: It was demonstrated that the 97 kDa molecule contained a complex of sugar chains bound to asparagine. Poly(A)^+-RNA was obtained from total RNA extracted from adult worms. Further analysis is currently underway if mrna of the gene coding for the 97 kDa molecule is contained in the RNA preparation.
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