• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on molecular organization, immunochemical characterization and biological activities of protein layers in bacterial surfaces

Research Project

Project/Area Number 62480155
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionThe University of Tokushima

Principal Investigator

KAWATA Tomio  The University of Tokushima, School of Medicine, 医学部, 教授 (60035368)

Co-Investigator(Kenkyū-buntansha) TAKEOKA Aya  The University of Tokushima, School of Medicine, 医学部, 教務員 (00116831)
YAMATO Masayuki  The University of Tokushima, School of Medicine, 医学部, 助手 (90210492)
KOGA Tetsuro  The University of Tokushima, School of Medicine, 医学部, 助手 (20093859)
TAKUMI Kenji  The University of Tokushima, School of Medicine, 医学部, 助教授 (90035428)
増田 邦義  徳島大学, 医学部, 助手 (60035483)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsBacterial surface / Cell wall / Protein layer / Molecular organization / Immunochemical characterization / Biological activity / Clostridium difficile / Clostridium botulinum / ラクトバシルス・アシドフィルス
Research Abstract

We have investigated the molecular organization, immunochemical properties, and biological activities of the regularly arranged proteins (RA proteins) in the outer cell well layers of some clostridia and lactobacilli. 1) The cell wall of clostridium difficile was revealed by electron microscopy to have an outer layer composed of a nearly square array and contained the two major proteins with Mrs of 32 and 45 kDa, or 38 and 42 kDa, depending on the strains. The amino acid composition and peptide maps of the two RA proteins differed from each other. Ca^<2+> was essential for the reassembly of regular array from the two RA proteins solubilized with urea or guanidine hydrochloride. The 32 kDa protein possessed the adhesive activity ot HeLa cells, whereas the 45 kDa protein did not. Immunoelectron microscopy revealed that the two RA proteins existed on the cell well surface. 2) A linearly arranged thin array was observed on the outer cell wall layer of Clostridium botulinum type E and composed of three major proteins with Mrs of 90, 60 and 25 kDa. The 90 and 60 kDa proteins were purified and immunochemically characterized. Both proteins were shown to locate on the wall surface by immunoelectron microscopy. 3) Regular arrays were present in only strains belonging to a group of lactobacillus acidophilus but not in the other groups. Based on the peptide mapping and immunoblotting of the RA proteins, the A group could be further classified into 4 or more subgroups.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report

Research Products

(21 results)

All Other

All Publications (21 results)

  • [Publications] 宅見賢二: Microbiology and Immunology. 31. 837-849 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 増田邦義: Microbiology and Immunology. 33. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宅見賢二: 日本細菌学雑誌. 43. 171 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 竹岡あや: 日本細菌学雑誌. 43. 394 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 古賀哲郎: 日本細菌学雑誌. 44. 320 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 竹岡あや: 日本細菌学雑誌. 44. 321 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] TAKUMI, Kenji: "Purification and immunochemical propertiesof a wall protein antigen from Clostridium difficile ATCC 11011" Microbiology and Immunology. 31. 837-849 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] MASUDA, Kuniyoshi: "Characterization and reassembly of a regular array in the cell wall of Clostridium difficile GAI 4131" Microbiology and Immunology. 33. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] TAKUMI, Kenji: "A regular array of cell wall in Clostridium botulinum type E" Japanese Journal of Bacteriology. 43. 171 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] TAKEOKA, Aya: "Cell wall proteins of Clostridium difficile and their adhesion to HeLa cell" Japanese Journal of Bacteriology. 43. 394 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] KOGA, Tetsuro: "Isolation, immunochemical properties and texonomic significance of regularly arranged proteins in cell walls of Lactobacillus acidophilus strains" Japanese Journal of Bacteriology. 44. 320 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 宅見賢二: Microbiology and Immunology. 31. 837-849 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 増田邦義: Microbiology and Immunology. 33. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宅見賢二: 日本細菌学雑誌. 43. 171 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 竹岡あや: 日本細菌学雑誌. 43. 394 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 古賀哲郎: 日本細菌学雑誌. 44. 320 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 竹岡あや: 日本細菌学雑誌. 44. 321 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Microbiology and lmmunology. 31. 837-849 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 竹岡あや: 日本細菌学雑誌. 43. 394 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 宅見賢二: 日本細菌学雑誌. 43. 171 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 古賀哲郎: 日本細菌学雑誌. 43. 230 (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi