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Molecular genetics of subactute sclerosing panencephalitis (SSPE) virus

Research Project

Project/Area Number 62480157
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

YAMANOUCHI Kazuya  Inst. Med. Sci., Univ. Tokyo, Professor, 医科学研究所, 教授 (30072888)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Yasuhiro  Inst. Med. Sci., Univ. Tokyo, Assoc. Professor, 医科学研究所, 助教授 (80109975)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsSSPE virus / Measles virus / Molecular genetics / Neurovirulence / Persistent infection / Neurotoxin / Pathogenesis / Slow virus infection / ブンガロトキシン / HA蛋白 / アセチルコリンレセプタ- / PーMmRNA / NPーL mRNA / 麻疹ウイルスts株
Research Abstract

(1) Gene analysis of SSPE virus, Yamagata-1 strain, was conducted. The entire base sequences of M, NP, H, and F genes as well as part of the sequences of L and P genes were determined.
(2) Di-cistronic mRNA of NP-L was most abundantly observed in cDNA library. It was classified into two types; the one consisting of 865 bp of NP and 1293 bp of L, and the other consisting of 187 bp of NP and 1192 bp of L. Such di-cistronic mRNA has not been reported thus far.
(3) The amino acid sequence of the carboxy terminus corresponding to the end of the outer envelope projection of HA of SSPE virus was found to have a high degree of homology with the active domain of long neurotoxin which specifically binds to the nicotini acetylcholine.receptor. This domain was shown to cross-react with HA of SSPE virus and of measles virus antigenically. In addition, SSPE patients had significantly high titers of antibody to this domain. Thus, this epitope of HA is considered to play an important role in the transsynaptic spreading of the virus in the brain.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] K.Tsukiyama et al.: "Molecular cloning and sequence analysis of the rinderpest virus mRNA encoding the hemagglutinin" Virology. 160. 48-54 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T.C.Wong et al.: "Generalized and localized biased hypermutation affecting the matrix gene of a measles virus strain that causes subacute sclerosing panencephalitis" J.Virol.63. 5464-5468 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis(SSPE)virus.I.Nucleotide sequence of the nucleoprotein." Virus Genes(In press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y.Yoshikawa et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis(SSPE)virus.II.Nucleotide sequence of a cDNA corresponding to the P plus M mRNA." Virus Genes(In press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis(SSPE)virus.III.Nucleotide sequence of the hemagglutinin gene." Virus Genes(In press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K.Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis(SSPE)virus.IV.Nucleotide sequence of the fusion gene." Virus Genes(In press). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Tsukiyama et al.: "Molecular cloning and sequence analysis of the rinderpest virus mRNA encoding the hemagglutinin" Virology, 160, 48-54, 1987.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Tsukiyama et al.: "Fusion glycoprotein (F) of rinderpest virus: Entire nucleotide sequence of the F mRNA, and several features of the F protein." Virology, 164, 523-530, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Imaoka et al.: "Induction of antinuclear antibodies in rabbits by infection with rinderpest virus with different degree of virulence." Jpn. J. Vet. Sci., 50, 1115-1118, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Imaoka et al.: "Characterization of antinuclear antibodies induced in rabbits by rinderpest virus infection." Arch. Virol., 102, 111-118, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Morikawa et al.: "Reversion of neurovirulence and in vitro phenotype of neural cell-adapted canine distemper virus after passage in non-neural cells." Microbiol. Immunol., 32, 1263-1268, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Morikawa et al.: "Characterization of canine distemper viruses adapted to human neural cells." Microbiol. Immunol., 32, 1211-1220, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Yoshikawa et al.: "Natural infection with canine distemper virus in a Japanese monkey (Macaca fuscata)." Vet. Microbiol., 20, 193-205, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. C. Wong et al.: "Generalized and localized biased hypermutation affecting the matrix gene of a measles virus strain that causes subacute sclerosing panencephalitis." J. Virol., 63, 5464-5468, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] M. Sugiyama et al.: "Characterization of monoclonal antibodies against four structural proteins of rinderpest virus." J. gen. Virol., 70, 2605-2613,.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Tsukiyama et al.: "Development of heat-stable recombinant rinderpest vaccine." Arch. Virol., 107, 225-235, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-strain of defective subacute sclerosing panencephalitis (SSPE) virus. I. Nucleotide sequence of the nucleoprotein gene." Virus Genes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Y. Yoshikawa et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. II. Nucleotide sequence of a cDNA corresponding to the P plus M dicistronic mRNA." Virus Genes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis (SSPE) virus. III. Nucleotide sequence of the hemagglutinin gene." Virus Genes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] K. Komase et al.: "Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis (SSPE) virus. IV. Nucleotide sequence of the fusion gene." Virus Genes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuhiro Yoshikawa: "Natural Infection with Canine Distemper Virus in a Japanese Monkey (Macaca fuscata)." Veterinary Microbiology. 20. 193-205 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 山内一也: "パラミクソウイルス" 最新醫學. 44. 1570-1573 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kyoko Tsukiyama: "Development of heat-stable recombinant rinderpest vaccine." Archives of Virology. 107. 225-235 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Makoto Sugiyama: "Characterization of Monoclonal Antibodies against Four Structural Proteins of Rinderpest Virus." J.gen.Virol.,. 70. 2605-2613 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Timothy C.Wong: "Generalized and Localized Biased Hypermutation Affecting the Matrix Gene of a Measles Virus Strain That Causes Subacute Sclerosing Panencephalitis" Journal of Virology. 63. 5464-5468 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 築山恭子: Virology. 164. 523-530 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 森川裕子: Microbiol.Immunol. 32. 1263-1268 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 森川裕子: Microbiol.Immunol.32. 1211-1220 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 吉川泰弘: ウイルス. 38. 23-57 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 山内一也: "新医科学大系第8巻A スローウイルス感染症" 中山書店, 27-49 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 築山恭子: Virology. 160. 48-54 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 山内一也: 免疫薬理. 5. 21-24 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 築山恭子: Virology. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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