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Study on the DNA replication of herpes simplex virus

Research Project

Project/Area Number 62480158
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNagoya University school of Medicine

Principal Investigator

NISHIYAMA Yukihiro  Nagoya University School of Medicine, 医学部, 助教授 (60115615)

Co-Investigator(Kenkyū-buntansha) TSURUMI Tatsuya  Nagoya University School of Medicine, 医学部, 講師 (90172072)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsHerpes simplex virus / DNA replication / DNA topoisomerase / DNA合成阻害剤 / DNA合成 / DNAトポイソメラーゼ / DNA複製
Research Abstract

The results of our experiments are summarized as follows.
1) The epipodophyllotoxin VP-16-213 and camptothecin have been shown to interfer with the functions of mammalian DNA topoisomerase II and I, respectively. Both inhibitors strongly suppressed the growth of herpes simplex virus. From our experiments using the two inhibitors, if was suggested that celluler DNA topoisomerase II and I are involved in the HSV replication; The former enzyme mainly plays a role in the viral DNA replication but the latter seems to primarily act at the level of transcription.
2) Aphidicolin-resistant or phosphonoacetic acid-resistant mutants of HSV have been isolated and characterized. The physical map limits for the Aph^r mutation were contained in a 1.1 Kbp region within the HSV DNA polymerase locus. It was shown that the Aph^r mutant polymerase had an amino acid substitution from a thyrosin to a histidine in the wellconserved region of the dna polymerase, DNA suggested that this region may form a domain that contains the binding-sites for substrates, PPi and aphidicolin.
3) We found that citrusinine-i, a new acridone alkaloid isolated from the root bark of citrus plant, inhibited HSV DNA synthesis at the concertrations that did not affect the synthesis of HSV-induced early proteins. Further studies will be required to determine the target of this compound.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yukihiro Nishiyama: Journal of Grneral.Virology. 68. 913-918 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tatsuya Tsurumi: Journal of Virology. 61. 388-394 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tatsuya Tsurumi: Gene. 52. 129-137 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizanur Rahman: Antiviral Research. 9. 11-22 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukihiro Nishiyama: Antimicrobial Ageuts and Chemotherapy. 32. 1053-1056 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 西山幸廣: 日本臨床. 47. 31-36 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukihiro,Nishiyama: "Effects of the epipodophyllotoxin VP-16-213 on herpes simplex virus type 2 replication" Journal of Geveral Virology. 68. 913-918 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tatsuya,Tsurumi: "A single-base changl within the DNA polymerase locus of herpes simplex virus type 2 can confer resistance to aphidicolin." Journal of Virology. 61. 388-394 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tatsuya,Tsurumi: "Nucleotide sequence of the DNA polymerase glne of herpes Simplex virus type 2 and comparison with HSV-1 counterpart." Gene. 52. 129-137 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Mizanur M. Rahman: "Inhibition of herpes simplex virus type 2 replication in vitroby 1-N-pentadecanoyl-3^Y-N-trifluoroacetyl-kanamy cin A." Avtiviral Research. 9. 11-22 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukihiro,Nishiyama: "Replication of herpes simplex virus DNA" Japanese Journal of Clinical Mediune. 47. 31-36 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yukihiro Nishiyama: Antimirobial Ageuts and chemotherapy. 32. 1053-1056 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Mizanur M.Rahman: Antivinal Research. 9. 11-22 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Eishi Takagi: Japanese Journal of Cancer Research(Gann). 79. 938-944 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Naohiko Yamomoto: Biochemical and Biophysical Research Communications. 158. 189-194 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hisashi Fujioka: Antimicrobial Ageuts and chemotherapy. 33. 6-9 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西山幸廣: 日本臨床. 47. 31-36 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yukihiro Nishiyama: Journal of General Virology. 68. 913-918 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tatsuya Tsurumi: Journal of Virology. 61. 388-394 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Naohiko Yamamoto: Biochemical and Biophysical Research Communications. 146. 1425-1431 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tatsuya Tsurumi: Gene. 52. 129-137 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 西山幸廣: ウイルス. 37. 55-70 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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