Tolerance induction of alloantigen-reactive T cells and analysis of its immunologic and cell-biologic mechanisms
| Project/Area Number |
62480165
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
FUJIWARA Hiromi Osaka Univ. Med. Sch. Associate Professor, 医学部, 助教授 (70116094)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAOKA Toshiyuki Osaka Univ. Med. Sch. Professor, 医学部, 教授 (60028529)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1988: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Transplantation immunity / immunologic tolerance / helper T cells / clonal deletion / ヘルパー型T細胞 / asialogly coprotein receptor |
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| Research Abstract |
This project aimed to (1) define conditions which induce the tolerance of helper/DTH type of T cells reactive to allo-antigens and (2) analyze immunologic and cell-biologic mechanisms underlying the tolerance.
We have found that: (1) the portal-venous adminiztration of allogeneic cells results in profound tolerance of alloantigen-reactive DTH T cells. The tolerance of these T cells was also inducible by i.v. administration of neuraminidase-treated allogeneic cells; (2) The above tolerance was mediated and/or maintained by a) induction of suppressor cell activity, b) generation of anti-idiotypic antibody and c) clonal elimination of alloreactive DTH T cells; (3) allo-class I H-2 reactive Lyt-2^+helper T cells were rendered tolerant by a single i.v. pre-sensitization with the corresponding allogeneic cells. This tolerance induction did neither require the treatment of allo-geneic cells with neuraminidase nor the injection via the portal venous route. It was also demonstrated that the prolonged survival of allo-class I H-2-disparate skin grafts was observed in such tolerant recipients.
These results provide approaches to eliminate alloreactivity and to attempt the prolongation of allograft survival.
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Report
(3 results)
Research Products
(21 results)
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[Publications] Mizushima, Y.; Kosaka, H.; Sakuma, S.; Kanda, K.; Itoh. K.; Osugi, T.; Mizushima, A.; Hamaoka, T.; Yoshida, H.; Sobue, K.; Fujiwara, H.: "Cyclosporin a inhibits late steps of T'lymphocyte activation after transmembrane signaling" J. Biochemistry. 102. 1193-1201 (1987)
Description
「研究成果報告書概要(欧文)」より
Related Report
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