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Analysis of liver cell-surface antigens in the process of the development of hepatocellular carcinoma with a monoclonal antibody

Research Project

Project/Area Number 62480199
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

MORIZANE Toshio  School of Med. Keio Univ. Internal Med Lecturer, 医学部・内科学, 専任講師 (20129703)

Co-Investigator(Kenkyū-buntansha) IWABUCHI Naoto  Scool of Med. Keio Univ. Internal Med., Assistant, 医学部・内科学, 助手 (90168592)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsHepatocellular carcinoma / Monoclonal antibody / Tumor-specific antigen / Tumor-specific antibody / 1-butanol extract / HCCM / 1ーbutanol extract / tumor-sepecific antigen / モノクローナル抗体 / 癌化 / 血清診断 / 肝特異抗原 / 肝細胞がん / 細胞膜抗原 / 肝細胞
Research Abstract

We have attempted to detect antibodies against tumor-specific antigens (TSA) of hepatocellular carcinoma (HCC) in sera from patients with HCC using a human hepatoma cell line, HCC-M, which we had established in our laboratory. we have also attempted to produce reurine monoclonal antibodies reactive with this cell line. Cell surface antigen molecules of HCC-M cells were extracted with 1-butanol extraction method from the suspension of living cells. An enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibodies to this extract. Antibody binding was detectable in sera from patients with liver cirrhosis (LC) as well as HCC. There was no statistically significant difference between these two diseases. However, after absorbing sera with a normal liver antigen preparation, it was possible to demonstrate HCC-specific antibody binding to the HCC-M butanol extract. Therefore, we thought that this antigen preparation contained TSA which was common in HCC.
we developed reurine monoclonal antibodies against the HCC-M butanol extract and obtained 1H-9 monoclonal antibody. It was demonstrated by immunoperoxidase technique and ELISA that 1H-9 monoclonal antibody was reactive with HCC-M cells, HCC tissues but not with cancer cells of other types. The reactivity of 1H-9 with normal liver cells was almost negligible. We developed an ELISA for determining the serum level of the corresponding antigen. It was higher in 19 out of 26 cases of HCC than normal range. This result suggests that the determination of the serum level of the antigen recognized by 1H-9 monoclonal antibody serves ad a diagnostic tool for HCC. The 1-butanol extract of HCC-M cells was divided into 17 fractions by a high pressure liquid chromatography and immune reactivities evaluated by leukocyte adherence inhibition assay and antibody binding assay were demonstrable in a small molecule fraction. These results also suggest that the 1-butanol extract of HCC-M contains TSA of HCC.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 佐藤逸郎: "Leukocyte adherence inhibition(LAI)反応からみた肝癌腫瘍特異抗原" 消化器と免疫. 18. 236-240 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 斉藤英胤: "マウスモノクロ-ナル抗体H2により規定されるヒト肝特異抗原の性状解析" 日本消化器病学会雑誌. 84. 909-914 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 森實敏夫: "肝細胞癌と免疫" 消化器と免疫. 21(suppl.). 21-35 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 森實敏夫: "ヒト肝特異抗体" 肝胆膵. 17. 689-696 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 岩渕直人: "慢性肝疾患患者末梢血単核球のin vitroにおける肝特異抗体産生の研究" 慶応医学. 66. 907-919 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 松村茂: "肝細胞癌患者血清中に検出された肝癌細胞抽出物(1-butanol extract)に対する抗体の研究" 慶応医学. 67. 1-14 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Saito H: "Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line,HCC-M" Viral Hepatitis and Hepatocellular Carcinoma.Sung J-L,ed.Taipei,ROC,. 48-61 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Satoh I: "Serological diagnosis of hepatocellular carcinoma with monoclonal antibody" Viral Hepatitis and Hepatocellular Carcinoma.Sung J-L,ed.Taipei,ROC,. 139-153 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Morizane T: "Effect of interferon-β on in vitro production of antibodies against hepatitis B virus and liver-specific idiotype-bearing antibody of peripheral blood mononuclear cells in chronic hepatitis B." The Biology of the Interferon System 1988.Kawade T and Kobayashi S,eds.Kodansha Scientific,Tokyo,. 91-96 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Saito H: "Decrase of transplantability by the immunopotentiators,OK-432 and interleukin-2:Experiments on a human hepatoma cell line in nude mice." Eur J Cancer Clin Oncol. 25. 79-89 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Saito H: "Establishment of a human cell line(HCC-T)from a patient with hepatoma bearing no evidence of hepatitis B or Avirus infection." Cancer. 64. 1054-1060 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Ding J-J: "Hepatitis B virus DNA integration in hepatocellular carcinomas and their adjacent non-neoplastic liver tissues." Keio J Med. 38. 443-453 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] I. Satoh, et al.: "Tumor-specific antigens of hepatocellular carcinoma defined by leukocyte adherence inhibition (LAI) assay." Shoukaki to Meneki 18 236-240, 1987 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Saito, et al.: "Characterization of human liverspecific antigen 1 (HLSA1), defined by the murine monoclonal antibody H2." Jpn J Gastroenterol 84 909-914, 1987 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Morizane: "Human liver-specific antibody." Kan Tan Sui 17 689-696, 1988 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] N. Iwabuchi: "Study of liver-specific idiotype bearing antibody production in vitro in patients with chronic liver disease." Keio Igaku 66 907-919, 1989 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] S. Matsumura: "The investigation of antibodies to extract (1-butanol extract) of hepatocellular carcinoma cells in sera of patients with hepatocellular carcinoma." Keio Igaku 67 1-14, 1990 (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Saito, et al.: "Transformation of NIH/3T3 cells by DNA from a human hepatoma cell line, HCC-M." Viral Hepatitis and Hepato-cellular carcinoma. Sung J-L., ed. 1988, Taipei, ROC, pp48-61.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] I Satoh, et al.: "Serological diagnosis of hepatocellular carcinoma with monoclonal antibody." ibid pp139-153.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] T. Morizane, et al.: "Effect of interferon- on in vitro production of antibodies against hepatitis B virus and liver-specific idiotype-bearing antibody of peripheral blood mononuclear cells in chronic hepatitis B." The Biology of the Interferon System 1988. Kawade T & Kobayashi S, eds., 1988, Kodansha Scientific Tokyo, pp91-96.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Saito, et al.: "Decrease of transplantability by the immunopotentiators, OK-432 and interleukin-2: Experiments on a human hepatoma cell line in nude mice." Eur J Cancer Clin Oncol 25 79-89, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] H. Saito, et al.: "Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or A virus infection." Cancer 64 1054-1060, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] J-J. Ding, et al.: "Hepatitis B virus DNA integration in hepatocellular carcinomas and their adjacent non-neoplastic liver tissues." Keio J Med 38 443-453, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 佐藤逸郎: "ヒト肝癌細胞培養HCCーMに対するモノクロ-ナル抗体1Hー9の反応性" 消化器と免疫. 17. 215-219 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] 松村茂: "肝細胞癌患者血清中に検出された肝癌細胞 抽出物(1ーbutanol extract)に対する抗体の研究" 慶應医学. 67. 1-13 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤逸郎: "Serological Diagnosis of Hepatocellular Carcinoma with monoclonal antibody" Proceedings of the lst International Symposium on Viral Hepatitis and Hepatocellular Carcinoma. 139-153 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] Watanabe,T.: "Establishment of a cell line(HCC-M)from a human hepatocellular carcinoma." Int.J.Cancer. 32. 141-146 (1983)

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤逸郎: "Ieukocyte adherene inhibition(LAI)反応からみた肝癌腫瘍特異抗原" 消化器と免疫. 18. 236-240 (1987)

    • Related Report
      1989 Annual Research Report
  • [Publications] 斉藤英胤 他: 日本消化器病学会雑誌. 84. 675-683 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hidetugu Saito 他: Cancer. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Toshio Morizawa 他: (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Itsurou Satoh 他: "Viral Hepatitis and Hepatocellnlar Carcinoma" Asia and Pacific Council for Science and Technology(Republic of Cluna), 139-153 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 斎藤英胤: 日本消火器病学会雑誌. 84. 675-683 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 岩渕直人: 消火器と免疫. 19. 163-166 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 森實敏夫: 医学のあゆみ. 143. 817-817 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 森實敏夫: European Journal of Cancer and Clinical Oncology. 23. 163-169 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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