| Project/Area Number |
62480228
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
MIKAWA Haruki Professor, Faculty of Medicine, Kyoto University, 医学部, 教授 (00026866)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AKIYAMA Yuichi Faculty of Medicine, Kyoto University, 医学部, 助手 (60175809)
HEIKE Toshio Faculty of Medicine, Kyoto University, 医学部, 助手 (90190173)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Project Status |
Completed (Fiscal Year 1988)
|
| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥4,200,000 (Direct Cost: ¥4,200,000)
|
|---|
| Keywords | Interferon / Asthma / Histamine / IgE受容体 / 2′,5′オリゴアデニレート / ヒスタミン遊離 |
|---|
| Research Abstract |
To investigate the effects of interferon on bronchial asthma, we investigated the regulatory effects of interferon on histamine release from human peripheral leukocytes and rat basophilic leukemia cells(2H3) and on Fc epsilon receptor 2(Fc epsilon R2/CD 23) expression on a human monocytic leukemia cell line (U937) and a human eosinophilic leukemia cell line EoL-3. Interferon increased the amounts of histamine released from those cells induced either by TNP-specific IgE and TNP-BSA of by calcium ionophore, in human peripheral leukocytes, interferon increased % release of intracellular histamine but did not increase the content of intracellular histamine. In 2H3 cells, interferon increased the intracellular histamine content by augmentation of histidine decarboxylase activity but did not increase the % histamine release. 2', 5'-oligoadenylates (2,5-A) showed the same effects as interferon on 2H3 cells and interferon induced 2,5-A synthetase activity in 2H3 cells. These data suggest that 2,5-a play an important role on interferon-induced increase of histamine release. Interferon also increased Fc epsilon R2 expression on U937 and EoL-3 cells. Interferon increased Fc epsilon R2 mrna in U937 cells determined by northern blot hybridization with the use of Fc epsilon R2 cDNA as a probe. All these data suggest that interferon, which may be induced by viral infections, may worsen asthmatic attacks by its enhancing effects on histamine release and on Fc epsilon R2 expression on monocytes and eosinophils, and prolonged asthmatic attack seen at the time of viral infection may be partly due to these effects of interferon.
|
