| Project/Area Number |
62480283
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Shimane Medical University |
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Principal Investigator |
NAKASE Akira Shimane Medical University,Professor, 医学部, 教授 (40025565)
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| Co-Investigator(Kenkyū-buntansha) |
NAITOH Atsushi Shimane Medical University,Assistant, 医学部, 助手 (80207688)
ONO Junichi Shimane Medical University,Assistant, 医学部, 助手 (90169334)
NOHARA Takahiko Shimane Medical University,Assistant, 医学部, 助手 (70180768)
YAMAMOTO Tsuyoshi Shimane Medical University,Assistant Professor, 医学部, 講師 (30135561)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | Intermittent Hepatic Artery Occlusion / Hepatocellular Carcinoma / Tc99m-MAA Scintigraphy / High Molar Salt-treated Autologous Plasma / 高張塩処理自己血漿 / ヌードマウス継代移植ヒト肝細胞癌 / 無血清下培養ヒト肝細胞癌細胞 / プロスタグランディンE^2 / 無血清下倍養ヒト肝細胞癌細胞 / プロスタグランディンE_2 / 癌免疫療法 / 血中CIC / 肝細胞癌培養細胞 / ヌードマウス可移植ヒト肝細胞癌 |
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| Research Abstract |
1. Clinical studies: A new methodology, surgical placement of a double lumen balloon catheter into the hepatic artery and intermittent arterial occlusion combined with infusion chemotherapy, was developed for treatment of unresectable tumors in the liver. Nineteen patients with primary hepatocellular carcinoma (HCC) and 14 with metastatic liver tumors were treated. Reductions in tumor sizes (50% or more) and one-year-survival rates (Kaplan-Meier) were 73.3% and 51.6% in cases of HCC and 35.7% and 23.1% in those of metastatic liver tumors, respectively. Hemodynamic studies using Tc99m-MAA revealed that HCC received 3.3 times more arterial blood than nontumor area, and that during inflation of the balloon, the ratio was increased to 8.8, though, in tumors, the area that received arterial blood was diminished. These findings may give a rationale for infusions of chemotherapeutic agents both during inflation and during deflation of the balloon. Eighteen patients at advanced stages of various types of malignancy received clinical trials of "antitumor response after reinfusion of high molar salt-treated autologous plasma". In 13 patients, beneficial changes as were proven by macroscopic or microscopic observations, or those in tumor markers were attained. Effectiveness of antitumor action seemed to exceed an algebraic sum of each treatment of chemotherapy or plasm-therapy alone when cytocidal agents were employed in combined manner with plasma infusion.
2.Experimental studies: a cell line of human HCC was established. The cells were able to be transferred in nude mice and cultured under serum-independent conditions, enabling studies on biological behaviors of HCC in vitro as well as in vivo. It was revealed that the cells were able to produce not only AFP and albumin but also transferrin, fibronection and prostaglandin E_2. Studies using nude mice revealed that indomethacin prolonged latent periods of transplanted HCC and achieved, in occasions, reductions in tumor sizes.
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