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Analysis of DNA contnto in malignant potential of colorectal cancer

Research Project

Project/Area Number 62480289
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKinki University

Principal Investigator

YASUTOMI Masayuki  Kinki University School of Medicine Professor, 医学部, 教授 (60028438)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Eiji  Kinki University School of Medicine Assistant, 医学部, 助手 (30210149)
KUROOKA Kazuyoshi  Kinki University School of Medicine Assistant, 医学部, 助手 (40195574)
SHINDO Katsuhisa  Kinki University School of Medicine Assistant Professor, 医学部, 助教授 (80112043)
MATSUDA Taiji  Kinki University School of Medicine Instructor, 医学部, 講師 (10122110)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordscolorectal cancer / DNA content / microspectrophotometry / flow cytometry / DNA ploidy pattern / malignato potential / prognosis of patient / Flow cytometry / DNA diploidy / DNA aneuploidy / DNA index / リンパ節転移 / 肝転移 / DNA heterogeneity / 悪性度 / 核DNA量histogram / 核DNA量ヒストグラム
Research Abstract

Study of nuclear DNA content and malignant potential in colorectal carcinoma.
1) Microspectrophotometric DNA analysis was carried out in 76 cases of colorectal carcinoma. In result four types of DNA histogram patterns were obtained : In type I, the peak ranged from 1.6 to 2.4c (diploidy) and the DNA content over 4c was distributed by less than 20%. In type II , the peak was also diploidy and the DNA content over 4c was distributed by 20% or more. In type III , the peak appeared in the range from 2.5c upward (aneuploidy) and one over 4c was found by less than 20%. In type IV , the peak was aneuploidy and the one over 4c was found by 20% or more. Diploidy case was observed in 85.0% of the 13 cases in early colorectal cancer, while in type IV it was observed in only one case(7.7%). Lymphatic permeation was remarkable (ly_<2.3>) in 5 cases (11.6%) with type I and in 24 cases (55.8%) with type IV . Venous invasion was remarkable (v_<2.3>) in one case (6.7%) with type I and in 8 cases (53.3%) with type IV .
2) Flow cytometric DNA analysis was carried out using paraffin-embedded materials of 143 cases of colorectal carcinoma. The ratio of DNA diploidy against DNA aneuploidy was one second. DNA ploidy pattern was not correlated with histological grade but with histological factors such as lymphatic permeation and venous invasion. Rate of lymphnode metastasis was higher in DNA aneuploidy (62.4%) than DNA diploidy (30.0%).
At the primary operation, the rate of liver metastasis in DNA aneuploidy was 16.4% but in DNA diploidy was not observed. These results indicate that DNA ploidy pattern is related to lymphnode metastasis and liver metastasis. Furthermore, the 5-year survival rates in patients with absolute curative resection were lower significantly in DNA aneuploidy (62.5%) than DNA diploidy(92.9%).
In conclusion microspectophotometric and flow cytometric DNA analyses are useful for evaluating the biological malignant potential and predicting the liver metastasis.

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 黒岡一仁: "核DNA量histogram patternからみた大腸癌の臨床病理学的研究" 近畿大学医学会雑誌. 13. 433-448 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 進藤勝久: "悪性度からみた大腸癌の診断と治療" 日本外科学会雑誌. 88. 1087-1092 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 黒岡一仁: "重複癌症例における核NDA量解析" フロ-サイトメトリ-. 7. 96-99 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 松田泰次: "大腸癌肝転移の防止対策" 外科. 51. 903-913 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 黒岡一仁: "大腸癌におけるDNA indexと再発形式に関する検討" FCM-Cell Biology(印刷中). 2. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 船井貞往: "Flow cytometryによる核DNA量からみた大腸癌の悪性度に関する検討-DNA ploidy patternと肝転移の関連性について-" 日本外科学会雑誌(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuyoshi Kurooka: "Clinicopathological study of DNA histogram pattern in colorectal cancer" Medical Journal of Kinki Univ. Vol. 13, 1988, 433-448.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Katsuhisa Shindo: "Diagnosis and treatment of large intestinal carcinoma from a viewpoint of the malignant potential" Journal of Japan Surgical Society Vol 88, 1987, 1087-1092.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuyoshi Kurooka: "Analysis of flow cytometry DNA histogram in double cancer" Flow cytometry Vol. 7, 1989, 96-99.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Taiji Matsuda: "The estimation and management of liver metastasis after colorectal surgery" Surgery Vol. 51, 1989 903-913.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kazuyoshi Kurooka: "Analysis of DNA index by flow cytometry and mode of recurrence in colorectal carcinoma" FCM - Cell Biology Vol. 2 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sadao Funai: "Flow cytometric DNA analysis of malignant potential in colorectal Carcinomas - DNA ploidy pattern and liver metastasis -" Journal of Japan Surgical Society.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 黒岡一仁: "重複癌症例における核DNA量解析" フロ-サイト・メトリ-. 7. 96-99 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 松田泰次: "大腸癌肝転移の防止対策" 外科. 51. 903-913 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 黒岡一仁: "大腸癌におけるDNA indexと再発形式に関する検討" FCM-Cell Biology. 2. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 船井貞往: "Flow cytometryによる核DNA量からみた大腸癌の悪性度に関する検討ーDNA ploidy patternと肝転移の関連性についてー" 日本外科学会雑誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 進藤勝久: 日本外科学雑誌. 88. 1087-1092 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒岡一仁: 近畿大学医学雑誌. 13. 433-448 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒岡一仁: フローサイトメトリー. 7. 96-99 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒岡一仁: 日本外科学会雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 黒岡一仁: 日本消化器外科学会雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] 進藤勝久: 日本外科学会雑誌. 88. 1087-1092 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 黒岡一仁: 近畿大学医学雑誌.

    • Related Report
      1987 Annual Research Report
  • [Publications] 黒岡一仁: フローサイトメトリー. 7. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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