| Project/Area Number |
62480308
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
MOGAMI Heitaro Osaka University Neurosurgery Professor, 医学部, 教授 (00028309)
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| Co-Investigator(Kenkyū-buntansha) |
ARITA Norio Osaka University Neurosurgery Associate Professor, 医学部, 助手 (80159508)
YAMADA Kazuo Osaka University Neurosurgery Associate Professor, 医学部, 助手 (90150341)
HAYAKAWA Toru Osaka University Neurosurgery Assistant Professor, 医学部, 助教授 (20135700)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | brain tumor / glioma / chemotherapy / growth factor / oncogene / epidermal growth factor / intrathecal chemotherapy / meningeal gliomatosis / brain tumor / glioma / astrocytoma / chemotherapy / growth factor / oncogene / epidermal growth factor receptor / astorocytoma |
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| Research Abstract |
1. Effect of a new morpholino anthracycline, MX2, against experimental brain tumors : MX2 is highly lipophilic and can cross blood brain barrier. (1)It showed a strong growth inhibitory action against human and rat glioma cells. (2)The survival time of rats with meningeal carcinomatosis was significantly prolonged by MX2. (3)The growth of subcutaneous glioma in rats was significantly retarded by MX2. (4)A significant amount of MX2 was detected in CSF after intravenous injection in normal rabbits.
2. Distribution of EGF receptor and amplification of erb B gene in human glioms : EGF receptors were immunohistochemically stained in 11 of 13 glioblastomas. Amplification of erb B gene was observed in 2 of 11 glioblastomas. Thus, the presence of EGF receptor on cells was not necessarily accompanied by amlification of erb B gene in human glioblastomas.
3. Sensitivity assay by in situ nick translation : Glioma cells after treated by antineoplastic agents having DNA damaging action had grains on t
… More
he nuclei in proportion to the drug concentration. This method can be applied to in situ sensitivity assay.
4. Loss of heterozygosity on chromosoiae 10 in human glionas : RFLPs analysis using polymorphic markers on chromosome 10 showed loss of heterozygosity in 6 of 14 glioblastomas. No loss of heterozygosity on chromosome 10 was observed in anaplastic astrocytomas or medulloblastomas. Loss of gene on chromosome 10 may play an important role in the tumorigenesis of glioblastomas.
5. Enhanced motility of malignant gliona cells to fibronectin and laninin : Migration of glioma cells was examined by a modified Boyden chamber assay method. The extracellular matrix, fibronectin and laminin, stimulated the directional migration of glioma cells. Thus, the extracellular matrix seems to be involved in the tissue invasion of malignant glioma cells through its action on the receptor of cell surface.
6. T98G human glioblastona cells have many nicks in DNA in the early Gl phase : T98G cells become arrested in the Gl phase when serumis deprived. In situ nick translation demonstrated that they have many nicks in DNA in the early Gl phase. The disappearance of nicks was delayed by the ihhibor of DNA topoisomerase II. Less
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