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Fundamental Studies to Elucidate Cerebral Developmental Impairment Mechanisms in Congenital Hydrocephalus and Development of Intrauterine Treatment.

Research Project

Project/Area Number 62480312
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionJuntendo University

Principal Investigator

SATO Kiyoshi  Department of Neurosurgery, Professor, 医学部脳神経外科, 教授 (10112707)

Co-Investigator(Kenkyū-buntansha) SUDA Kikuo  Department of Neurosurgery, Assistant Researcher, 医学部脳神経外科, 助手 (00206559)
TAKEDA Nobuaki  Department of Neurosurgery, Assistant Researcher, 医学部脳神経外科, 助手 (00171645)
ITO Masanori  Department of Neurosurgery, Assistant Professor, 医学部脳神経外科, 講師 (20095764)
MIYAOKA Makoto  Department of Neurosurgery, Assistant Professor, 医学部脳神経外科, 講師 (70053270)
宮澤 隆仁 (宮沢 隆仁)  順天堂大学, 医学部・脳神経外科, 講師 (80182012)
石井 昌三  順天堂大学, 医学部・脳神経外科, 教授 (80052926)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordscongenital hydrocephalus / congenital hydrocephalic rat / ventriculography / magnetic resonance imaging / ^<14>C-deoxyglucose / Golgi study / ventriculoperitoneal shunt / cerebral cortex / 脳室腹腔短絡術 / 大脳皮質 / ゴルジ法 / シナプス / 螢光抗体法 / 学習脳 / 大脳皮貭 / ゴルヂ法 / 蛍光抗体法 / Y迷路
Research Abstract

We have experimentally treated the hydrocephalus of congenitally hydrocephalic HTX-rats by inserting a ventriculoperitoneal shunt (V-P shunt), at different times after birth, investigated through neuropathology and neurochemistry clarification of how timing of such surgery modifies impairment in development of neurocytes and synaptogenesis, and assessed the learning ability of the experimental animals.
Experimental results obtained in 1987 suggested that placement of the V-P shunt delayed until around 4 weeks after birth of the hydrocephalic animals did not correct impairment of synaptogenesis of cortical pyramidal neurons and learning disability in comparison with non-hydrocephalic HTX-rats. In light of such observations, earlier placement of the V-P shunt at around 7 days after birth was carried out in experiments conducted in 1988 and 1989. The significance of the early placement was evaluated by various means, i.e., (1) chronological changes in lateral ventricle size and thickness o … More f the cortical mantle of the brain by means of Isovist ventriculography and magnetic resonance imaging(MRI), (2) measurement by 14C-deoxyglucose autoradiography of local cerebral glucose utilization (LCGU), (3) neuropathological techniques including rapid Golgi staining, (4) immunohistochemical assessment of synaptogenesis using monoclonal antibodies against a synaptic vesicle protein (SVP-38 of Obata et al., 1986), and (5) observation of learning ability by means of the Y-Arm-Maze Test.
The results revealed that early placement of the V-P shunt was obviously effective in correcting reduced width of the cortical mantle and dilated ventricular size, mitigating cerebrospinal fluid white matter edema, normalizing the shape and arrangement of the ependymal cells lining the ventricular walls, preventing impairment of LCGU, restoring spine density of cerebral pyramidal neurons, and in decreasing learning disability. The present investigation would appear to have established a theoretical base supporting the necessity for early placement of a V-P shunt in animals with congenital hydrocephalus.
Furthermore, the results may urge reconsideration of intrauterine treatment of congenital hydrocephalus in humans which a majority of neurosurgeons already have abandoned. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 宮澤隆仁他: "先天性水頭症ラット-HTX-における大脳皮質神経細胞の発達障害について-Golgi study-" 小児の脳神経. 13. 263-270 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮岡誠他: "先天性水頭症ラットに対するV-Pシャント前・後における脳局所グルコ-ス代謝" 小児の脳神経. 13. 389-393 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wada M.: "Congenital hydrocephalus in HTX-rats:incidence,pathophisiology,and developmental impairment." Neurologia medico-chirurgica. 28. 955-964 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyaoka M.etal: "Measurement of local cerebral glucose utilization befor and after V-P shunt in congenital hyrocephalusin rats." Metabolic Brain Disease. 3. 125-132 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazawa T.et al: "A new method of quantitatively analyzing synapsis using a synaptic vesicle protein(SVP-38)monoclonalantibody." in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazawa t.et al: "Impairment of synaptogenesis and learning disability in theHTX-rats with arrested sunt-dependent hydrocephalus." in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 佐藤潔他句: "水頭症-基礎と臨床-先天性水頭モデルの病態(出版予定)" 松本 悟(永井書店),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Wada M: "Congenital hydrocephalus in HTX-rats: incidence, pathophysiology, and developmental impairment." Neurologia medico-chirurgica 28, 955-964, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyaoka M. et al: "Measurement of local cerebral glucose utilization before and after V-P shunt in congenital hydrocephalus in rats." Metabolic Brain Disease 3, 125-132, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazawa T. et al: "A new method of quantitatively analyzing synapses using a synaptic vesicle protein (SVP-38) monoclonal antibody."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazawa T. et al: "Impairment of synaptogenesis and learning disability in the HTX-rats with arrested shunt-dependent hydrocephalus."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyazawa T. et al: "A quantitative Golgi study of cortical pyramidal neurons in congenitally hydrocephalic rats-HTX." Nervous System in Children 13, 263-270, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Miyaoka M. et al: "The effect of V-P shunt on local cerebral glucose utilization in HTX congenital hydrocephalic rats." Nervous System in Children 13, 389-393, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 宮澤隆仁 他: "先天性水頭症ラットーHTXーにおける大脳皮質神経細胞の発達障害についてーGolgi studyー" 小児の脳神経. 13. 263-270 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮岡誠 他: "先天性水頭症ラットに対するVーPシャント前・後における脳局所グルコ-ス代謝の変化" 小児の脳神経. 13. 389-393 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Wada M.: "Congenital hydrocephalus in HTXーrats:incidence,pathophisiology,and developmental impairment." Neurologia medicoーchirurgica. 28. 955-964 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyaoka M.et al: "Measurement of local cerebral glucose utilization before and after VーP shunt in congenital hydrocephalus in rats." Metabolic Brain Disease. 3. 125-132 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyazawa T.et al: "A new method of quantitatively analyzing synapses using a synaptic vesicle protein(SVPー38)monoclonal antibody."

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyazawa T.et al: "Impairment of synaptogenesis and learning disability in the HTXーrats with arrested shuntーdependent hydrocephalus."

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤潔 他: "水頭症ー基礎と臨床ー先天性水頭症モデルの病態" 松本悟(永井書店),

    • Related Report
      1989 Annual Research Report
  • [Publications] Wada,M.: Neurol Med Chir(Tokyo). 28. 955-964 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Miyaoka,M.et al: Metabolic Brain Disease. 3. 125-132 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 宮澤隆仁 他: 小児の脳神経. 13. 263-270 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 佐藤潔 他: CLINICAL NEUROSCIENCE.

    • Related Report
      1988 Annual Research Report
  • [Publications] 北川康行, 田中信夫, 勝部幸輝, 楠木正巳 他: Neurologia medico-chirurgica. A40. 35 (1985)

    • Related Report
      1987 Annual Research Report
  • [Publications] Makoto Miyaoka.etal: Metabolic Brain Disease. 99. 1289-1298 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] 北川康行, 田中信夫, 畑安雄, 勝部幸輝, 佐藤能雅: 小児の脳神経. B43. 272-275 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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