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Treatment of lung metastasis from renal cell carcinoma with lymphokine-activated killer cells and interleukin-2.

Research Project

Project/Area Number 62480336
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionYamanashi Medical College

Principal Investigator

UENO Akira  Yamanashi Medical College, Dept. of Urology, 医学部, 教授 (30010193)

Co-Investigator(Kenkyū-buntansha) KOMATSU Hideki  Yamanashi Medical College, Dept. of Urology, 医学部, 助教授 (30167407)
TASAKA Kachio  Yamanashi Medical College, Dept. of Immunology, 医学部, 助教授 (40093265)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsrenal cell carcinoma / lung metastasis / adoptive immunotherapy / lymphokine-activated killer cell / lymphokine-activated killer cell / 腎癌 / LAK細胞 / インターロイキン2
Research Abstract

The conditions to induce lymphokine-activated killer (LAK) cells were investigated at first. Concentrations of serum in culture media, 1, 2 and 5% had no effect on induced LAK activity. The best density of peripheral blood lymphocytes in culture bottle was 2x10^6/ml, and the most effective concentration of recombinant interleukin-2 (<@2r<@D2IL-2) to generate LAK cells was 3u/ml. Cytotoxicity against Daudi cell appeared on the second day of culture and reached a peak on the third day. There was no difference in induced LAK activity between standing and rolling culture.
One patient with lung metastasis from renal cell carcinoma was treated with LAK cells and <@2r<@D2IL-2. LAK cells and <@D2r@>D2IL-2 were administered 11 times during 14 months, the first 2 times intravenously and 9 times via bronchial artery. The total number of LAK cells injected was 16.1x10<@D19@>D1 cells. Induced LAK activities gradually decreased and reached minimum at 6th treatment. From the 6th treatment plasma exchange was performed the day before administration of LAK cells. Induced LAK activities recovered from the 7th treatment. Despite these treatments the size of the metastatic lesion gradually increased.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Hideaki Iizuka: Yamanashi Medical Journal. 3. 97-103 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 田坂捷雄: クリニカル ファーマシー. 15. 41-44 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 薮崎昇: Yamanashi Medical Journal.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirofumi Naganuma: Acta neurochirurgica.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hideaki Iizuka: "Sudy or lymphokine-activated killer (LAK) cells. I Improved LAK cell induction in vitro" Yamanashi Medical Journal. 3. 97-103 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kachio Tasaka: "Tumor immunotherapy with LAK cells." Clinical Pharmacy. 4. 41-44 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Noboru Yabusaki: "Adoptive immunotherapy for lung metastasis from renal cell carcinoma." Yamanashi Medical Journal.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hirofumi Naganuma: "Complete remission of recurrent glioblastoma multiforme following infusions of lymphokine-activated killer cells. A case report" Acta Neurochirurgia.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hideaki Iizuka: Yamanashi Medical Journal. 3. 97-103 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 田坂捷雄: クリニカルファーマシー. 15. 41-44 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 薮崎昇: Yamanashi Medical Journal.

    • Related Report
      1988 Annual Research Report
  • [Publications] Hirofumi Naganuma: Acta-Neurochirurgica.

    • Related Report
      1988 Annual Research Report
  • [Publications] Hidehiko Ishizuka;Hirofumi Naganuma;Noboru Yabusaki;Hideki Komatsu;Yasuyoshi Yamamoto;Hideki Fujii;Masayuki Yamanoto;Yoshiroh Matsumoto;Katsuhiko Sugahara;Hideki Nukui;Akira Ueno;Kachio Tasaka: 山梨医科大学雑誌.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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