| Project/Area Number |
62480351
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
NOZAWA Shiro Keio Univ., School of Med., Assist. Prof., 医学部・産婦人科, 講師 (90051557)
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| Co-Investigator(Kenkyū-buntansha) |
UDAGAWA Yasuhiro The 2nd Tokyo National Hosp., Staff, 産婦人科, 医員 (80118918)
MUKAI Makio Keio Univ., School of Med., Assist. Prof., 医学部・病理, 講師 (50101895)
KIGUCHI Kazushige Keio Univ., School of Med., Assist. Prof., 医学部・産婦人科, 講師 (60101911)
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| Project Period (FY) |
1987 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1988: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1987: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Glycolipids / Endometrium / Sulfatide / Sulfotransferase / Monoclonal antibody / Flow cytometry / 血液型糖鎖 / ヒト型モノクロ-ナル抗体 / I型糖鎖 / マウス子宮内膜 / ステロイドホルモン / GA1,GM3,Sulfatide / Forssman抗原 / モノクローナル抗体MSN-1,MSN-2,フローサイトメトリー / 子宮腔内抗原測定 / ヒト子宮内膜 / マウス型MSNー1抗体 / 血液型物質ルイスb / ヒト型HMSTー1抗体 / パラグロボシド / フローサイトメトリー |
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| Research Abstract |
Since glycolipids on the cell membrane act as a factor in cell recognition, their counterparts in the endometrium may play an important role in implantation. Because sugar chain of glycolipids have been shown to change after malignant transformation, glycolipids may serve as an important diagnostic marker of endometrial cancer. Therefore, we decided to analyze the composition of these molecules from normal and malignant endometric with the aim of developing a new diagnostic method for detection of endometrial cancers.
We found that sulfatide, one of the acidic glycolipids, is present in normal endometrial cells and that its amount in the secretory phase is greater than that in the proliferative phase, suggesting that such glycolipid may be involved in implantation. Furthermore, we found that sulfotransferase activity, which is necessary for sulfatide synthesis, is present in endometrial cancer cells; which may indicate that sex steroid hormones have something to do with the gene expression of this enzyme.
We developed two monoclonal antibodies against endometrial cancers. One of them, MSN-1, reacts with the Le^b-related sugar moiety of mouse glycolipid; and the other, HMST-1, reacts with human glycolipid type I sugar chain. We applied MSN-1 to flow cytometry to develop a diagnostic method for endometrial cancer, and found that this monoclonal seldom reacted with normal endometrial cells but was reactive with neoplastic cells in 70% of the endometrial cancer cases examined. Our data indicate that this method is very useful for the diagnosis of endometrial cancers.
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