• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

In vitro differentiation of human osteosarcoma cells

Research Project

Project/Area Number 62480374
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionFaculty of Dentistry, Tokyo Medical and Dental University

Principal Investigator

TSUCHIDA Nobuo  Dept. of Microbiol., Fac. of Dent., Tokyo Med. Dent. Univ., 歯学部, 教授 (60089951)

Co-Investigator(Kenkyū-buntansha) SHIMOKAWA Hitoyata  Dept. of Biochemistry, Fac. of Dent., Tokyo Med. Dent. Univ., 歯学部, 助教授 (80014257)
FUTAESAKU Yutaka  School of Hygene, Kitasato University, 衛生学部, 教授 (50014197)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsosteosarcoma / alkalinephosphatase / 分化 / アルカリフォスファターゼ / オステオネクチン / グラ蛋白質 / ハイドロキシアパタイト / 分化誘導 / cDNAクローン
Research Abstract

Histopathological and electron microscopical observations suggest that the pattern of bone formation in osteosarcoma cells is principally similar to that of normally calcifying tissue. Using osteosarcoma cell line (HOS) established from human osteosarcoma, in the present work we asked questions whether or not (1) HOS cells is induced to differentiate in vitro and (2)KHOS cells, HOS cells transformed by Kirsten murine sarcoma virus (K-MSV) express differentiation markers of osteogenic cells. In addition, we studied on osteonection gene which is expressed in osteogenic cells.
(1) The expression of differentiation markers. HOS cells were found to express the bone/liver/kidney-type alkaline phosphatase(B/K/L/ALPase). The expression of ALPase was found to be regulated by growth rate and/or cellular contact. The addition of beta-glycerophosphate and ascorbic acid to the culture medium induced Hos cells to deposition of calcium inside. The electron microscopic observation showed that HOS cells produced hydroxyapatite (HA) crystals, which was identified by Debye-Scherrer ring and micro X-ray analyses. Two initiation sites for calcification were observed as vesicles with HA crystals inside; one type similar to matrix vesicle was 0.3-1 um in diameter and the other 1-3 um. We also observed development of HA crystals along collagen fibers. These results suggest that HOS cells possess an osteogenic potential and thus can be induced to express differentiation markers typical of osteogenic cells.
(2) The expression of differentiation markers in KHOS cells. The expression of both ALPase and deposition of calcium was suppressed. KHOS cells contained a single copy of K-MSV proviral integrated DNA from which RNA was transcribed. Therefore, it is highly likely that k-ras oncogene suppressed the expression of differentiation markers.
(3) We molecularly cloned osteonection cDNA from human and bovine and determined nucleotide sequences.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 池田通: 日腔病学会雑誌. 55. 106-118 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 片山奈知子: 口腔病学会雑誌. 55. 585-598 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tohru Ikeda: "Differentiation and calcification of human osteosarcoma cell line HOS." The Journal of the Stomatological Society, Japan. 55. 106-118 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nachiko Katayama: "The suppression of osteogenic differentiation markers in human osteosarcoma cells transformed by Kisrsten murine sarcoma virus." The Journal of the Stomatological Society, Japan.55. 585-598 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 池田通: 口腔病学会雑誌. 55. 106-118 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 片山奈知子: 口腔病学会雑誌. 55. 585-598 (1988)

    • Related Report
      1988 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi