• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Calcium channel alterations coupled with secretory response in rat parotid gland cells

Research Project

Project/Area Number 62480379
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

ISHIDA Hajime  The Univ. of Tokushima Sch. of Dentistry; Professor, 歯学部, 教授 (70028364)

Co-Investigator(Kenkyū-buntansha) WATANABE Shinji  The Univ. of Tokushima Sch. of Dentistry; Research Associate, 歯学部, 助手 (00201187)
KODA Naohiko  The Univ. of Tokushima Sch. of Dentistry; Research Associate, 歯学部, 助手 (60144995)
ISHIKAWA Yasuko  The Univ. of Tokushima Sch. of Dentistry; Assistant Professor, 歯学部, 講師 (40144985)
Project Period (FY) 1987 – 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1989: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1988: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1987: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsSalivary Glands / Amylase Secretion / Calcium Channel / Calcium Antagonists / Drug Receptors / Phosphatidylinositol / Inositol 1, 4, 5-Triphosphate / [^3H] Nitrendipine / 耳下腺 / アセチルコリン / イソプロテレノ-ル / イノシト-ル1,4,5ー三燐酸 / アミラーゼ分泌 / カルシウム / IP_3・GTP / カルシウム拮抗薬
Research Abstract

It is well known that calcium ion plays an important for role in the regulation of the physiological function of cells. In salivary glands, calcium ion activates the intracellular processes and causes the secretory response. Calcium channel is considered to be very important to regulate the intracellular calcium concentration. We have studied characteristics of the calcium channel in rat parotid ,land cell in relation to secretory response.
Amylase was secreted from rat parotid gland cells by the incubation oil them in the medium containing a calcium ionophore, A23187, or in the medium containing high concentration of K^+. The stimulation by isoproterenol of beta-adrenoceptors or acetylcholine of muscarinic receptors of rat parotid gland cells caused also amylase secretion from the cells. The presence of an adequate concentration of extracellular calcium was necessary for the amylase secretion from rat parotid gland cells. Release of amylase from secretory granules, zymogen granules, in … More rat parotid gland cells was also regulated by a low concentration of calcium. The contents of inositol 1, 4, 5-triphosphate in rat parotid gland dispersed cells were increased about 80% by the incubation of them in the presence of acetylcholine for 5 min. at 37゚C. These results showed that calcium ion liberated from an intracellular store of parotid gland cells has a stimulatory effect on amylase secretion. Amylase secretion from rat parotid gland cells caused by acetylcholine or isoproterenoll was not blocked by nifedipine, nicardipine, verapamil, or diltiazem. Binding experiments of [^3H]nitrendipine with rat parotid gland cell membrane did not show the presence of specific binding sites.
These results clarify that the increases in the intracellular concentration of calcium ion entered into rat parotid gland cells through the calcium channel activated by drug receptors and liberated from an intracellular store in the cells by inositol 1, 4, 5-triphosphate play an important role in amylase secretion from the cells. Less

Report

(4 results)
  • 1989 Annual Research Report   Final Research Report Summary
  • 1988 Annual Research Report
  • 1987 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 石川康子: "ラット唾液腺アデニル酸シクラ-ゼの生後変化" 歯科基礎医学会雑誌(印刷中). 32. ( ) (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Decreased single transduction in rat parotid cell aggregates during aging is not due to loss of alpha_1-adrenergic receptor" Experimental Gerontology. 24. 25-36 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 幸田直彦: "ラット唾液腺核蛋白質のりん酸化の加齢に伴う動態" 歯科基礎医学会雑誌. 31. 291-301 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fumiaki Hata: "Dual effects of clonidine on amylase secretory responeses of rat parotid tissue induced by β-and α_1-agonists" Japanese Journal of Pharmacology. 46. 217-224 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Malcolm V.Gee: "Impaired adrenergic stimulation of rat parotid cell glucose oxidation during aging:The role of calcium" Journal of Gerontology. 41. 331-335 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fumiaki Hata: "Dopamine-induced amylase secretion from rat parotid salivary gland in vitro:an effect mediated via noradrenergic and cholinergic nerves" British Journal Pharmacology. 89. 473-483 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石田甫: "歯科薬理学,消化器系に作用する薬物" 医歯薬出版株式会社, 11 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石田甫: "歯科薬理学,平滑筋に作用する薬物" 医歯薬出版株式会社, 6 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Developmental alterations of adenylate cyclase in rat parotid glands" Japanese Journal of Oral Biology 32, 1990.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Yasuko Ishikawa: "Decreased single transduction in rat parotid cell aggregates during aging is not due to loss of alpha_1-adrenergic receptor" Experimental Gerontology 24, 25-36, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Naohiko Koda: "Age-dependent changes in the phosphorylation of nuclear phosphoproteins of rat salivary glands" Japanese Journal of Oral Oral Biology 31-3, 291-301, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fumiaki Hata: "Dual effects of clonidine on amyase secretory responses of rat parotid tissue induced by beta - and alpha_1 -agonists" Japanese Journal of Pharmacology 46, 217-224, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Malcolm V. Gee: "Impaired adrenergic stimulation of rat parotid cell glucose oxidation during aging : The role of calcium" Journal of Gerontology 41, 331-335, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Fumiaki Hata: "Dopamine-induced amylase secretion from rat parotid salivary gland in vitro : an effect via noradrenergic and cholinergic nerves" British Journal Pharmacology 89, 473-483, 1986.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hajime Ishida: Drugs acting on the gastrointestinal tracts. Dental Pharmacology Ishiyaku Publishers, Inc. 3rd ed., 178-188 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Hajime Ishida: Drugs acting on the smooth muscle. Dental Pharmacology Ishiyaku Publishers, Inc. 3rd ed., 213-218 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 石川康子: "ラット唾液腺アデニル酸シクラ-ゼの生後変化" 歯科基礎医学会雑誌. 32. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Diurnal rhythm of phosphorylation of nuclear non-histone proteins of rat parotid glands"

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuko Ishikawa: "Decreased signle transduction in rat parotid cell aggregates during aging is not due to-loss of alpha_1-adrenergic receptor" Experimental Gerontology. 24. 25-36 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 幸田直彦: "ラット唾液腺核蛋白質のりん酸化の加齢に伴う動態" 歯科基礎医学会雑誌. 31. 291-301 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiaki Hata: "Dual effects of clonidine on amylase secretory responses of rat parotid tissue induced by β-and α_1-agonists" Japanese Journal of Pharmacology. 46. 217-224 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Malcolm V.Gee: "Impaired adrenergic stimulation of rat parotid cell glucose oxidation during aging:The role of calcium" Journal of Gerontoloty. 41. 331-335 (1986)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石田甫: "歯科薬理学 消化器系に作用する薬物" 医歯薬出版株式会社, 11 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 石田甫: "歯科薬理学 平滑筋に作用する薬物" 医歯薬出版株式会社, 6 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumiaki Hata;Etsuo Kondo;Hajime Ishida: British Journal Pharmacology. 89. 473-483 (1986)

    • Related Report
      1988 Annual Research Report
  • [Publications] Fumiaki Hata;Etsuo Kondo;Hajime Ishida: Japanese Journal of Pharmacology. 46. 217-224 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yasuko Ishikawa;Malcom V.Gee;Indu S.Ambudkar;Lipa Bonder;Bruce J.Baum;George S.Roth: Biochemica et Biophysica Acta. 968. 203-210 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 石川康子,渡邊晋二,陳倉,石田甫: 歯科基礎医学会雑誌. 30. Suppl.125 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 幸田直彦,陳倉,崔勇,石野康子,石田甫: 歯科基礎医学会雑誌.

    • Related Report
      1988 Annual Research Report
  • [Publications] Masahiro Yoshiyama;Jiro Masada;Akitsugu Uchida;Hajime Ishida: Journal of Dental Research.

    • Related Report
      1988 Annual Research Report
  • [Publications] Fumiaki Hata;Etsuko Kondo;Hajime Ishida: British Journal Pharmacology. 89. 473-483 (1986)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hajime Ishida;Yasuko Ishikawa;Jiro Masada;Masahiro Yoshiyama: xth International Congress of Pharmacology. 5 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yasuko Ishikawa;Jiro Masada;Shinji Watanabe;Hajime Ishida: '87The international Symposium of neurotransmitter receptors. 69 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Yasuko Ishikawa;Atuko Nosaka;Shinji Watanabe;Jiro Masada;Naohiko Koda;Hajime Ishida: Japanese Journal of Pharmacology. Suppl.208 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Naohiko Koda;Yasuko Ishikawa;Jiro Masada;Atsuko Nosaka;Hajime Ishida: Japanese Journal Pharmacology. Suppl.314 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Fumiaki Hata;Etsuo Kondo;Hajime Ishida: Japanese Journal of Pharmacology. 46. 217-224 (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi