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Roles of lysosomal proteinases in the patho-physiological changes of hard tissues.

Research Project

Project/Area Number 62480381
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

KATO Yuzo  Nagasaki University School of Dentistry, 歯学部, 教授 (20014128)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Miho  Nagasaki University School of Dentistry, 歯学部, 助手 (50191341)
YAMAMOTO Kenji  Nagasaki University School of Dentistry, 歯学部, 助教授 (40091326)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsLysosomal proteinases / Cathepsin B / Cathepsin H / Cathepsin D / Cathepsin E / カテプシンL / メダラシン / リソゾーム酵素 / 抗炎症剤
Research Abstract

Recent studies have suggested that a variety of lysosmal proteinases play an importantrrole in destructive lesion of hard tissues. However, a fundamental question regarding the mechanism of destructive process of hard tissue proteins still remains to be answered. The present studies was undertaken to prepare various lysosomal proteinases and antibodies specific for these enzymes for defining the properties of degradative system responsible for the breakdown of the tissue proteins. In the fiscal year of 1987, the work was concerned chiefly with lysosomal cysteine proteinases cathepsin B and cathepsin H. Preparation of the pure enzymes and their antibodies was accomplished in this year. Comparative analyses with various protease inhibitors and anti-inflammatory agents indicated the difference in catalytic site properties between cathepsins B and H. In the fiscal year of 1988, attention was focused on intracellular aspartic proteinases cathepsin D and cathepsin E. Immunochemical and immunohistochemical studies revealed that these two enzymes had the different distribution and subcellular localization in various tissues and blood cells. cathepsin d was detected in all of the tissues and cells tested, whereas cathepsin E had a relatively limited distribution. In addition, cathepsin D was associated with the lysosome-rich fraction, whereas cathepsin E behaved as a soluble cytosolic enzyme.
All through the two years, possible roles of lysosomal proteinases in pathological destructive process of periodontal tissues were investigated. The results obtained from analyses of the gingival crevicular fluid from chronic adult periodontitis patients who had rediographic evidence of alveolar bone loss indicated that the levels of cathepsins B, H, L and medullasin was correlated

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Yamamoto,Kenji: Biochem.Biophys.Res.Commun.148. 267-272 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 国松和司: 日本歯周病学会会誌. 29. 1094-1101 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto,Kenji: Biol.Chem.Hoppe-Seyler. 369. 315-322 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山本健二: 炎症. 8. 217-222 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山本健二: 日本薬理学会誌. 91. 371-378 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Jupp.Ray: Biochem.J.254. 895-898 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto,Kenji: J.Biochem.105. 114-119 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Thomas,J.: FEBS Lett.(1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 国松和司: 炎症. 9. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 山本健二: 炎症. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakai,Hideaki: Biochim.Biophys.Acta.105. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ueno,Eiko: J.Biochem.105. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 加藤有三: "鎮痛抗炎症剤の副作用" 国際医書出版(歯科ジャーナル), 755-760 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto,Kenji: "Intracellular Protein Catabolism" Springer-Verlag & Japan.Sci.Soc.Press., (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "Biochemical and immunochemical similarity between erythrocyte membrane aspartic proteinase and cathepsin E." Biochem. Biophys. Res. Commun.148. 267-272 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunimatsu, Kazushi: "Studies on the roles of lysosomal proteinases in periodontal breakdown." J. Japan. Ass. Periodont.29. 1094-1101 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "An aspartic proteinase of erythrocyte membranes. Proposed mechanism for activation and further molecular properties." Biol. Chem. Hoppe-Seyler. 369. 315-322 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "Erythrocyte membrane aspartic proteinase(EMAP). Properties and biological controls." Jpn. J. Inflam.8. 217-222 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "Differential effects of non-steroidal anti-inflammatory drugs on cathepsins D and E from rat spleen." Folia pharmacol. japon.91. 371-376 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Jupp, Ray: "Identification of the slow-moving proteinase from human gastric mucosa and the aspartic proteinase of erythrocyte membranes as catalytically equivalent to cathepsin E." Biochem. J.254. 895-898 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "Age-related and phenylhydrazine-induced activation of the membrane-associated cathepsin E in human erythrocytes." J. Biochem.105. 114-119 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Thomas, J.: "Stabilization of cathepsin E by ATP." FEBS Lett.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunimatsu, Kazushi: "Cathepsins B, H and L activities in gingival crevicular fluid from patients with chronic adult periodontitis." Jpn. J. Inflam.9. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: "Gingival crevicular fluid level of medullasin, an elastase-like serine proteinase." Jpn. J. Inflam.9. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Sakai, Hideaki: "Quantitation and immunohistochemical localization of cathepsins E and D in rat tissues and blood cells." Biochim. Biophys. Acta. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Ueno, Eiko: "Activation mechanism for erythrocyte cathepsin E. Evidence for the occurrence of membrane-associated active enzyme." J. Biochem.105. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kato, Yuzo: "Hypocalcemic action of several types of salicylic acid analogues." Jpn. J. Oral Biol.31. 89-94 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kato, Yuzo: Side Effects of Analgesic Anti-Inflammatory Drugs.Kokusai Isho Shuppan, 755-760 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto, Kenji: Intracellular Protein Catabolism.Springer-Verlag & Japan. Sci. Soc. Press, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Yamamoto Kengi: Biol Chem Hoppe-Seyler. 369. 315-322 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 山本健二: 炎症. 8. 217-222 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 山本健二: 日本薬理学会雑誌. 91. 371-376 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamamoto Kengi: J.Biochem. 105. 114-119 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Kato Yuzo: Jpn J.Oral Biol.31. 89-94 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sakai Hideaki: Biochim.Biophys.Acta. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamamoto Kenji: "Intracellular Protein Catabolism" Springer-Verlag Japan Sci Soc Press, (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Yamamoto Kenji: Biockem.Biophrs.Res.Commun.148. 267-272 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 加藤有三: 歯科ジャーナル. 26. 755-760 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 国松和司: 日本歯周病学会会誌. 29. 1094-1101 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 山本健二: 炎症. 8. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] 山本健二: 日本薬理学雑誌. 91. (1988)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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