| Project/Area Number |
62480385
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
OKADA Hiroshi Osaka Univ. Faculty of Dentistry ・ Professor, 歯学部, 教授 (40038865)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAUCHI Hidetoshi Osaka Univ. Faculty of Dentistry ・ Research Assistant, 歯学部, 助手 (70187425)
HARADA Yasushi Osaka Univ. Faculty of Dentistry ・ Research Assistant, 歯学部附属病院, 助手 (10181025)
MATSUO Takashi Osaka Univ. Faculty of Dentistry ・ Research Assistant, 歯学部, 助手 (30173800)
KIMURA Shigenobu Osaka Univ. Faculty of Dentistry ・ Associate Professor, 歯学部附属病院, 講師 (10177917)
EBISU Shigeyuki Osaka Univ. Faculty of Dentistry ・ Associate Professor, 歯学部, 助教授 (50116000)
葛西 康宏 大阪大学, 歯学部, 助手 (60177417)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Immunological study / Periapical lesion / 免疫学的研究 / 免疫担当細胞 / インターロイキンー1 / 慢性根尖性歯周炎 |
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| Research Abstract |
Recent observations suggested that human periapical lesions developed as a result of immunopathological responses to continuous antigenic stimuli from infected root canals. The purpose of this study was to clarify the involvement of immune mechanisms in the pathogenesis of periapical lesions.
First, we investigated the property of infected root canal contents from a clinical point of view in terms of antigenicity. It revealed that some qualities of root canal contents, namely wet condition, black pigmentation and foul odors, were closely related to existence of periapical lesions. These findings suggested that bacteria might be the crucial antigenic substance in the canal contents. Therefore microorganisms in the root canals were investigated. Results showed anaerobic gram-negative rods including bacteroides species had closely relation to the existence of periapical lesions. Consequently, anaerobic gram-negative rods were important as the antigenic stimuli in development of periapical
… More
lesions.
Second, we characterized the immunocompetent cells in periapical lesions by using immunohistochemical methods, and examined their correlation with clinical findings. Results showed the proportion of these immunocompetent cells related to some clinical findings. These observations suggested that immune responses closely related to the pathosis of periapical lesions.
Finally, we measured the concentration of immunoglobulins and Interleukin-1(IL-1) in the periapical exudates of infected root canals by enzyme-linked immunosorbent assay and examined the correlation of these immune components to clinical findings. Results showed that IL-1 and immunoglobulines detected in periapical exudates were related with some clinical findings. Moreover, as the healing of periapical lesions, an increase of the level of IL-1 and a decrease of the levels of IL-1 , IgG and IgA were observed. Consequently immune responses in periapical lesions varied according to the pathosis of periapical lisions.
Above findings suggested immune responses played a important role in the pathogenesis of the periapical lesions. Less
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