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Hemolymph Coagulation and Defence Systems in Invertebrate Animals

Research Project

Project/Area Number 62480453
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionKyushu University

Principal Investigator

IWANAGA Sadaaki  Faculty of Science, Kyushu University, Professor, 理学部, 教授 (90029942)

Co-Investigator(Kenkyū-buntansha) MIYATA Toshiyuki  Faculty of Science, Kyushu University, Research Associate, 理学部, 助手 (90183970)
中村 隆範  日本学術振興会, 特別研究員(PD) (70183887)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1988: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1987: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsHemolymph Coagulation / Horseshoe Crab Hemocyte / Lipopolysaccharide / Factor C / _2-GP-I domain / EGF domain / Tachyplesin / セリンプロテアーゼチモーゲン / リポ多糖(内毒素) / β_2-GP-I様ドメイン / コアギュローゲン / リポ多糖感受性セリンプロテアーゼチモーゲン(C因子) / 補体因子 / 酸性リン脂質 / スロンビン / ホスファチジルグリセロール
Research Abstract

The horseshoe crab clotting factor, factor C, present in the hemocytes is a serine-protease zymogen activated with lipopolysaccharide. It is a two-chain glycoprotein (M_r = 123,000) composed of a heavy chain (M_r = 80,000) and a light chain (M_r = 43,000) [T. Nakamura et al. (1986) Eur. J. Biochem. 154, 511-521]. In our continued study of this zymogen, we have now also found a single-chain form of factor C (M_r = 123,000) in the hemocyte lysate. the heavy chain had the NH_2-terminal sequence of Ser-Gly-Val-Asp-, consistent with that of the single- chain factor C, indicating that the heavy chain is derived from the NH_2-terminal part of the molecule. The light chain had an NH_2-terminal sequence of Ser-Ser-Gln-Pro-. Incubation of the two-chain zymogen with lipopolysaccharide resulted in the cleavage of a Phe-Ile bond between residues 72 and 73 of the light chain. Concomitant with this cleavage, the A (72 amino acid residues) and B chains derived from the light chain were formed. The complete amino acid sequence of the A chain was determined by automated edman degradation. The A chain contained a typical segment which is similar in sequence to a family of repeats in human _2-glycoprotein I, complement factors B, protein H, C4b-binding protein, and coagulation factor XIII b subunit. The NH_2-terminal sequence of the B chain was Ile-Trp-Asn-Gly-. This chain contained the serine-active site sequence-Asp-Ala-Cys-Ser-Gly-Asp-Ser-Gly-Gly-Pro-.
These results indicate that horseshoe crab factor C exists in the hemocytes in a single-chain zymogen from and is converted to an acitve serine protease by hydrolysis of a specific Phe-Ile peptide bond.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Muta,T.: J.Biochem.101. 1321-1330 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tokunaga,F.: Eur.J.Biochem.167. 405-416 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,T.: J.Biochem.101. 1297-1306 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,T.: J.Biochem.103. 370-374 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,T.: Eur.J.Biochem.176. 89-94 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,T.: J.Biol.Chem.263. 16709-16713 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 岩永貞昭他2名編集: "ビタミンK:医学・生物学領域における新展開" メディカル・ジャーナル社, 332 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 岩永貞昭: "続・生化学実験講座(日本生化学会編)17章 血液凝固系総論" 東京化学同人, 8 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Muta, T. et al.: "Primary Structure of Anti-lipopolysaccharide Factor from American Horseshoe Crab, Limulus polyphemus." J. Biochem.101. 1321-1330 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Tokunaga, F. et al.: "Lipopolysaccharide-sensitive serine Protease Zymogen (factor C) of Horseshoe Crab Hemocytes: Identification and Alignment of Proteolytic Fragments Produced during the acitvation Show That It is a Novel Type of Serine-Protease." Eur. J. Biochem.167. 405-416 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura, T. et al.: "Interaction between Lipopolysaccharide and Intracellular Serine Protease Zymonge, Factor C, from Horseshoe Crab (Tacnypleus tridentatus) Hemocytes." J. Biochem.103. 370-374 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura, T. et al.: "Intracellular Serine-Protease Zymogen, Factor C, from Horseshoe Crab Hemocytes: Its Activation by Synthetic Lipid A Analogues and Acidic Phospholipids." Eur. J. Biochem.176. 89-94 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura, T. et al.: "Tachyplesin, a Class of Antimicrobial Peptide from the Hemocytes of the Horseshoe Crab (Tachypleus tridentatus)." J. Biol. Chem.263. 16709-16713 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Iwanaga, S. et al.: Vitamin K: New Horizons in Biomedical Research. Medical Journal, Co. Ltd., 332 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Nakamura,T.: J.Biochem.103. 370-374 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakamura,T.: Eur.J.Biochem.176. 89-94 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Nakamura,T.: J.Biol.Chem.263. 16709-16713 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takeya,H.: J.Biol.Chem.263. 13868-14877 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Tokunaga,F.: J.Biol.Chem.263. 17471-17481 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Sueyoshi,T.: J.Bilchem.103. 730-734 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 岩永貞昭 他: "ビタミンK:医学・生物学領域における新展開" (株)メディカル・ジャーナル社, 1-332 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 西村仁 他: "実験医学・特集" (株)羊土社, 1236-1243 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Muta,T.: J.Biochem.101. 1321-1330 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Tokunaga,F.: Eur.J.Biochem.167. 405-416 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakamura,T.: J.Biochem.101. 1297-1306 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Nakamura,T.: J.Biochem.(1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyata,T.: Biochemistry. 26. 1117-1122 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Miyata,T.: J.Biochem.102. 93-101 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 岩永貞昭: "続生化学実験講座(日本生化学会編)17章,血液凝固系総論" 東京化学同人, 8 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 宮田敏行: "続生化学実験講座(日本生化学会編)6章,自動エドマン分解" 東京化学同人, 21 (1987)

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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