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Enzymological study on inorganic nitrogen assimilation during redifferentiation of moss cells

Research Project

Project/Area Number 62540507
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

TAKAMI Shinji  Faculty of Science, Hiroshima University, Professor, 理学部, 教授 (60033809)

Co-Investigator(Kenkyū-buntansha) TAKIO Susumu  Faculty of Science, Hiroshima University, Assistant, 理学部, 助手 (60188109)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsCell cultures of bryophytes / Glutamate dehydrogenase / Glutamate synthase / Glutamine synthetase / Inorganic nitrogen assimilation / グルタミン酸生成酵素 / 硝酸還元酵素 / 蘚類培養細胞 / 再分化 / 亜硝酸還元酵素 / グルタミン酸合成酵素
Research Abstract

In recent years, we have established cell suspension cultures from the mosses, Barbula Unguiculata (Takio et al, 1986), Sphagnum imbricatum (Kajita et al. 1987) and Hedwigia ciliata (Takami and Takio 1987), the liverworts, Marchantia paleacea var. diptera (Takio et al. 1988) and Heteroscyphus beschesellei (Takami and Takio 1987), and the hornwort, Anthoceros punctatus (Takami et al. 1988). In contrast to cultures cells from higher plants, cell of most bryophyte species could not grow in a medium containing nitrate as the sole nitrogen source, while their parental or redifferentiated plants could grow in this medium. In order to elucidate the relatioship between differentiation and nitrogen metabolism, in vitro activities of nitrogen assimilating enzymes were determined during redifferentiation of S. imbricatum cells into whole plants.
Appropriste conditions for measuring the activities of nitrate reductase (NaR), glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) were determined in extraxts from suspension cultured cells of S. imbricatum. Pyridine nucleotide specificity and appropriate pH of GDH from S. imbricatum cells were different from those of other plant species. Catalytic properties of NaR, GS and GOGAT from S. imbricatum were similar to those from other plants.
Activities of GS, GOGAT and GDH were determined in extracts from callus cells and redifferentiated plantlets. There was no difference in activities of GS, GOGAT between both extracts. High activity of GDH was detected in extracts from callus cells, while no activity was observed in that from plantlets.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 高見伸治: Journal of Plant Physiology. 130. 267-272 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 梶田美幸: Physiologia Plantarum. 70. 21-26 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高見伸治: Physiologia Plantarum. 73. 227-231 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高見伸治: Journal of Hattori Botanical Laboratory. 64. 429-435 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 滝尾進: Journal of Plant Physiology. 132. 195-198 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 滝尾進: Journal of Plant Physiology. 132. 470-473 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shinji, Takami: "Establishment of cell suspension cultures of Hedwigia ciliata and Heteroscyphus bescherellei." Journal of Plant Physiology. 130. 267-272 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Miyuki, Kajita: "Establishment and growth characterization of suspension culture of cells from the moss, Sphagnum imbricatum." Physiologia Plantarum. 70. 21-26 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shinji, Takami: "Photoautotrophic growth in suspension culture of cells from the moss, Barbula unguiculata." Physiologia Plantarum. 73. 227-231 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Shinji, Takami: "Establishment of suspension cultures of cells from the hornwort, Anthoceros punctatus L." Journal of Hattori Botanical Laboratory. 64. 429-435 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Susumu, Takio: "Photosynthetic ability of dark-grown Marchantia paleacea cells in suspension culture." Journal of Plant Physiology. 132. 195-198 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 高見伸治: Physiologia Plantarum. 73. 227-231 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 高見伸治: Journal of Hattori Botanical Laboratory. 64. 429-435 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 滝尾進: Journal of Plant Physiology. 132. 195-198 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 滝尾進: Journal of Plant Physiology. 132. 470-473 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Takami, S.;Takio, S.: Journal of Plant Physiology. 130. 267-272 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kajita, M.;Takio, S.;Takami, S.;Hino, S.: Physiologia Plantarum. 70. 21-26 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Takio, S.: Journal of Hattori Botanical Laboratory. 62. 269-280 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Takami, S.;Nishikawa, S.;Takio, S.;Hino, S.: Physiologia Plantarum.

    • Related Report
      1987 Annual Research Report
  • [Publications] Takami, S.;Yasunaga, M.;Takio, S.;Kimura, J.;Hino, S.: Journal of Hattori Botanical Laboratory.

    • Related Report
      1987 Annual Research Report
  • [Publications] Takio, S.;Takami, S.;Hino, S.: Journal of Plant Physiology.

    • Related Report
      1987 Annual Research Report

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Published: 1987-04-01   Modified: 2016-04-21  

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