| Project/Area Number |
62540537
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Faculty of Medicine, University of Tokyo |
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Principal Investigator |
SHIODA Masaki Faculty of Medicine, University of Tokyo, 医学部, 助手 (80134526)
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| Project Period (FY) |
1987 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1988: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | DNA replication / Rate of DNA replication / Initiation site / Machinery for DNA replication / Development / ウニ胚 / 発生 / 微小管会合蛋白質 / 核マトリックス / DNA複製開始点 / DNA複製部位 |
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| Research Abstract |
The mechanism which regulates the rate of DNA replication during sea urchin embryogenesis was examined in respect of initiation sites of DNA replication and machinery for DNA replication.
It was found that many initiation sites for DNA replication are present on a chromosomal DNA in clusters in which the sites are arrenged at 1.2 kb intervals in embryos at the early developmental stage, whereas few initiation sites are present on the chromosomal DNA in embryos at the late developmental stage. These results suggest that the rate of DNA replication is regulated through the number of initiation sites for DNA replication. Furthermore, we found a factor which stimulates DNA synthesis in vitro. The number of the factor changed with the rate of DNA replication during embryogenesis. These results suggest that the rate of DNA replication is also regulated through the number of the factor during embryogenesis.
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