• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

STUDY ON CELL MOTILITY UPON FERTILIZATION AND CELL DIVISION BY MEANS OF MICROMANIPULATION

Research Project

Project/Area Number 62540538
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

HAMAGUCHI Yukihisa  TOKYO INSTITUTE OF TECHNOLOGY, FACULTY OF SCIENCE, 理学部, 助教授 (70016161)

Co-Investigator(Kenkyū-buntansha) 浜口 みやこ  東京工業大学, 理学部, 技官 (60192275)
HAMAGUCHI Miyako  TOKYO INSTITUTE OF TECHNOLOGY, FACULTY OF SCIENCE (70016161)
Project Period (FY) 1987 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1988: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsChromosome movement / Cortical granule breakdown / Micromanipulation / Mitotic apparatus / Monoclonal antibody / Nuclear migration / 単クローン抗体 / 分裂装置 / イトマキヒトデ卵 / ウニ卵 / ヒストン / 細胞 / 受精 / 細胞分裂 / 卵
Research Abstract

1. Cortical granule breakdown of sea urchin eggs was analyzed with a differential interference microscope equipped with a high-speed video system at 200 fields per second. At first, the diameter of cortical granules increased and then they disappeared gradually. The time required for the breakdown process was 30-40 msec even upon fertilization, after injection of a Ca buffer solution at high Ca^<2+> concentration into the eggs, and after perfusion of a solution at high Ca^<2+> concentration to the isolated cortex.
2. When fluorescently labeled calf thymus histone was injected into starfish oocytes, chromosomes could be visualized at maturation division and cleavage. When DNA synthesis was inhibited with aphidicolin, chromosomes moved to the equator but did not move to the poles during anaphase and through telophase at the first cleavage. This was confirmed by staining DNA and microtubules with DAPI and anti-tubulin antibody, respectively. After the second cleavage, the mitotic apparatus composed only of two asters without the spindle or the chromosomes formed in the blastomeres, which had divided repeatedly.
3. Female pronuclear migration upon fertilization is microtubule-dependent motility. When the microtubules connected from the sperm aster to the female pronucleus were cut with a microneedle by micromanipulation, the pronucleus stopped for a while and soon began to move. When the central part of the sperm aster was sucked into a micropipet, the pronucleus did not stop.
4. The mitotic apparatus was observed after staining with several monoclonal antitubulin antibodies by indirect immunofluorescence. The mitotic stage was determined by observing chromosomes with a differential interference microscope. There were four groups of monoclonal antibodies against tubulin, which stained all parts of the mitotic apparatus, only the spindle, and only the mitotic asters, and did not stain the mitotic apparatus at all.

Report

(3 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hamaguchi,Y.: Cell Structure Function. 12. 43-52 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜口幸久: 生物物理. 27. 262-267 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜口幸久: 生体の科学. 39. 102-105 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi,Y.: Zoological Science. 5. 545-552 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi,Y.: Cell Motility Cytoskeleton. 9. 153-163 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi,Y.: Cell Structure Function. 14. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi, Y.; M. Toriyama H.Sakai; Y. Hiramoto: "Redistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol." Cell Struct. Funct.12. 43-52 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi, Y.: "Dynamic changes in cytoskeleton of sea urchin eggs during early development." Seibutsubutsuri. 27. 262-267 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi, Y.; I. Mabuchi: "Accumulation of fluorescently labeled actin in the cortical layer in sea urchin eggs after fertilization." Cell Motil. Cytoskeleton. 9. 153-163 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi, Y.: "Translocation of chromosomes." Seitai no Kagaku. 39. 102-105 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Hamaguchi, Y.: "In vivo cytochemistry in cell division." Zool. Sci.5. 545-552 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 浜口幸久: 生物物理. 27. 262-267 (1987)

    • Related Report
      1988 Annual Research Report
  • [Publications] 浜口幸久: 生体の科学. 39. 102-105 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hamaguchi,Y.: Zoological Science. 5. 545-552 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hamaguchi,Y.: Cell Motility Cytoskeletou. 9. 153-163 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hamaguchi,Y.: Cell Structure Function. 14. (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 馬渕一誠, 浜口幸久: 生体の科学. 38. 501-503 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 浜口幸久: 生物物理. 27. 262-267 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Hamaguchi, Y. ;Mabuchi, I.: Cell Motility and the Cytoskeleton. 9. (1988)

    • Related Report
      1987 Annual Research Report

URL: 

Published: 1987-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi